Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
, 2010 In this paper, we describe the development of a bath comprising triethanolamine and sodium dodecyl sulfate for electrodeposition of CuInSe2 thin films, by which long-term bath stability was found to be improved and near-stoichiometric CuInSe2 films with ...
Yu, Rui +5 more
core +1 more source
APPLICATION DETERMINE THE CONCENTRATION OF SODIUM DODECYL SULFATE AFTER INTERCALATION OF LAYERED REM HYDROXIDES [PDF]
, 2020 Determination sodium dodecyl sulfate was applied for solution before intercalation of layered REM hydroxides. Method was prolonged for highly concentrated solution (10-70 g/l).Исследование выполнено за счет гранта Российского научного фонда (проект № 18 ...
Gordeyev, E. V. +5 more
core
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
FEBS Open Bio, EarlyView.Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
FEBS Open Bio, EarlyView.Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
Effect of sodium dodecyl sulfate (SDS) on barite flotation.
Effect of sodium dodecyl sulfate (SDS) on barite flotation.
Wenyu Meng (18155890) +9 more
core +1 more source
Growth of \u3ci\u3eEnterobacter cloacae\u3c/i\u3e in the Presence of 25% Sodium Dodecyl Sulfate [PDF]
, 1980 The growth of Enterobacter cloacae in 25% sodium dodecyl sulfate is described. The bacteria appeared to tolerate sodium dodecyl sulfate rather than metabolize it. The process was energy dependent, and cell lysis occurred during stationary phase.
Calabrese, Diane M. +2 more
core
Effect of counter ion concentration added with mixture of water and ethylene glycol on Krafft temperature of sodium dodecyl sulfate [PDF]
, 2007 Surfactants are amphiphilic organic compounds having both hydrophobic and hydrophilic groups. Surfactants in solution form aggregates known as micelles. The minimum temperature at which surfactants forms micelles is known as Krafft temperature.
Chundru, Sateesh Krishna Chand
core +1 more source
Comparison of Same Carbon Chain Length Cationic and Anionic Surfactant Adsorption on Silica
Colloids and Interfaces, 2020 Adsorption of a cationic surfactant dodecyl pyridinium chloride (DPC) on silica was studied to show a comparison with the adsorption of an anionic surfactant sodium dodecyl sulfate (SDS), whose carbon chain length is the same and on the same silica ...
Pengxiang Li +3 more
doaj +1 more source