HSP70 governs permeability and mechanotransduction in primary human endothelial cells
FEBS Open Bio, EarlyView.HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez +5 more
wiley +1 more source
Determination of the thermodynamic parameters and critical micelle concentration in the water-toluene-sodium dodecyl sulfate emulsion system in the presence of methyl methacrylate [PDF]
شیمی کاربردی روز, 2014 In this study, the effect of methyl methacrylate on the stability of the emulsion system and its distribution in water- toluene system, half decay time, surface tension by drip method and critical micelle concentration by conductometry method were ...
Fariborz Azizinezhad +2 more
doaj +1 more source
Micelle fragmentation and wetting in confined flow
, 2014 We use coarse-grained molecular-dynamics (MD) simulations to investigate the structural and dynamical properties of micelles under non-equilibrium Poiseuille flow in a nano-confined geometry.
Denniston, Colin +2 more
core +1 more source
Double-walled carbon nanotube dispersion via surfactant substitution [PDF]
, 2009 A new approach for the stabilisation of double-walled carbon nanotubes in aqueous media was developed. A low molecular weight surfactant was used in the first stage for the debundling of the nanotubes followed by substitution with a higher molecular ...
Datsyuk, Vitaliy +6 more
core +3 more sources
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
, 2018 NYS IPM Type: Minimum Risk PesticideLauryl sulfate, otherwise known as hydrogen dodecyl sulfate, is an anionic surfactant. It is not commonly used in pesticides, with sodium lauryl sulfate being the preferred form.
Baker, Brian P., Grant, Jennifer A.
core
Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae. [PDF]
, 1988 The application of two-dimensional electrophoretic procedures to structural and genetic studies of seed proteins from Poaceae (including the cultivated cereals) and Fagaceae is described.
Aragoncillo Ballesteros, Cipriano +5 more
core +2 more sources
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Terminal deoxynucleotidyltransferase. Serological studies and radioimmunoassay [PDF]
, 1976 Mouse antisera against calf terminal deoxynucleotidyltransferase (terminal transferase) have been prepared. The sera have been used to characterize terminal transferase both by studying inhibition of enzyme activity and by developing a competition ...
Baltimore, David +2 more
core
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source