Results 81 to 90 of about 1,614,546 (322)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Applicability of mitotic figure counting by deep learning: a development and pan‐cancer validation study

FEBS Open Bio, EarlyView.
In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes, Maria X. Isaksen, Frida Julbø, Manohar Pradhan, Andreas Kleppe, Sepp De Raedt, Ole‐Johan Skrede, Turid Torheim, John Arne Nesheim, Hans Martin Mohn, Hanne A. Askautrud, Karolina Cyll, Wanja Kildal, Emma Rewcastle, Melinda Lillesand, Vebjørn Kvikstad, Emiel Janssen, Robert Jones, Odd Terje Brustugun, Bjørn Brennhovd, Erik Skaaheim Haug, Lill‐Tove Rasmussen Busund, Elin Richardsen, Sigve Andersen, Tom Dønnem, Kristina Lindemann, Gunnar Kristensen, Neil A. Shepherd, Marco Novelli, Knut Liestøl, David Kerr, Håvard E. Danielsen, Tarjei S. Hveem   +32 more
wiley   +1 more source

Modeling Enterprise Authorization: A Unified Metamodel and Initial Validation

Complex Systems Informatics and Modeling Quarterly, 2016
Authorization and its enforcement, access control, have stood at the beginning of the art and science of information security, and remain being crucial pillar of security in the information technology (IT) and enterprises operations.
Matus Korman, Robert Lagerström, Mathias Ekstedt   +2 more
doaj   +1 more source

Formalization and visualization of domain-specific software architectures [PDF]

This paper describes a domain-specific software design system based on the concepts of software architectures engineering and domain-specific models and languages.
Bailor, Paul D., Luginbuhl, David R., Robinson, John S.   +2 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Hypermedia-based software architecture enables Test-Driven Development. [PDF]

JAMIA Open, 2023
Post AR, Ho N, Rasmussen E, Post I, Cho A, Hofer J, Maness AT, Parnell T, Nix DA.   +8 more
europepmc   +1 more source

Architectural Tactics in Software Architecture: A Systematic Mapping Study - Study protocol

, 2022
Gastón Márquez, Hernán Astudillo, Rick Kazman   +2 more
openalex   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

PlantPathNet: a novel network based on cross-layer feature integration for plant disease classification

Frontiers in Plant Science
IntroductionReliable identification of plant diseases from leaf images is essential for effective crop monitoring and the prevention of yield deterioration.
Demet Parlak Sönmez, Şafak Kılıç, Şafak Kılıç   +2 more
doaj   +1 more source

Entropy as a Measure of Consistency in Software Architecture. [PDF]

Entropy (Basel), 2023
Niepostyn SJ, Daszczuk WB.
europepmc   +1 more source