Results 41 to 50 of about 176,621 (285)

Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis [PDF]

, 2008
Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial ...
A. Gorg, C. Tastet, D. Mokranjac, F. Gharahdaghi, F. Granier, H. Zischka, J. C. Sanchez, M. Chevallet, M. Eschenbruch, P. Lescuyer, S. Iwata, S. Luche, S. Luche, S. Richert, T. Rabilloud, T. Tsukihara, V. Santoni   +16 more
core   +6 more sources

NaCl Modifies Biochemical Traits in Bacterial Endophytes Isolated from Halophytes: Towards Salinity Stress Mitigation Using Consortia

Plants
Bacterial endophytes (120) were isolated from six halophytes (Distichlis spicata, Cynodon dactylon, Eragrostis obtusiflora, Suaeda torreyana, Kochia scoparia, and Baccharis salicifolia). These halophiles were molecularly identified and characterized with
Jesús Adrián Barajas González, Yersaín Ely Keller de la Rosa, Rogelio Carrillo-González, Ma. del Carmen Ángeles González-Chávez, María Eugenia Hidalgo Lara, Ramón Marcos Soto Hernández, Braulio Edgar Herrera Cabrera   +6 more
doaj   +1 more source

Low Molecular Weight Chitosan-Insulin Complexes Solubilized in a Mixture of Self-Assembled Labrosol and Plurol Oleaque and Their Glucose Reduction Activity in Rats

Marine Drugs, 2018
Oral insulin delivery that better mimics physiological pathways is a necessity as it ensures patient comfort and compliance. A system which is based on a vehicle of nano order where positively charged chitosan interacts with negatively charged insulin ...
Amani M. Elsayed, Aseel H. Khaled, Mayyas M. Al Remawi, Nidal A. Qinna, Hussam Abu Farsakh, Adnan A. Badwan   +5 more
doaj   +1 more source

Properties of chicken liver membrane-associated thiamine triphosphatase [PDF]

The Ukrainian Biochemical Journal, 2015
The enzymes involved in thiamine triphosphate (ThTP) metabolism in birds are not characteri­zed so far. The aim of the present work was to study some properties of ThTPase in chicken liver.
I. K. Kolas, A. F. Makarchikov
doaj   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Improvement in Solubility–Permeability Interplay of Psoralens from Brosimum gaudichaudii Plant Extract upon Complexation with Hydroxypropyl-β-cyclodextrin

Molecules, 2022
Psoralen (PSO) and 5-methoxypsoralen (5-MOP) are widely used drugs in oral photochemotherapy against vitiligo and major bioactive components of root bark extract of Brosimum gaudichaudii Trécul (EBGT), previously standardized by LC-MS.
Rúbia Darc Machado, Júlio C. G. Silva, Luís A. D. Silva, Gerlon de A. R. Oliveira, Luciano M. Lião, Eliana M. Lima, Mariana C. de Morais, Edemilson C. da Conceição, Kênnia R. Rezende   +8 more
doaj   +1 more source

Purification and Characterization of a Chloroplast Outer-Envelope-Bound, ATP-Dependent Protein Kinase [PDF]

, 1988
An ATP-dependent protein kinase was partially purified from isolated outer envelope membranes of pea (Pisum sativum L., Progress No. 9) chloroplasts. The purified kinase had a molecular weight of 70 kilodaltons, as determined by sodium dodecyl sulfate ...
Soll, Jürgen
core   +2 more sources

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Mechanochemical solid acid/base reactions for obtaining biologically active preparations and extracting plant materials

Green Chemistry Letters and Reviews, 2017
Mechanochemically assisted extraction involves the mechanical treatment of powder mixtures of plant materials and convenient reagents in special mill-activators to solubilize the biologically active substances.
Oleg I. Lomovsky, Igor O. Lomovskiy, Denis V. Orlov   +2 more
doaj   +1 more source

Revisiting the Solubility–Permeability Relationship with Hydrophobic Drug Umifenovir in Pluronic Solutions: Impact of pH and Co-Solvent

Pharmaceutics, 2023
This study describes the influence of pluronic F-127 (F-127) and ethanol (EtOH) on the solubility of umifenovir (UMF) in buffer solutions of pH 2.0 and pH 7.4, and its permeability through cellulose membranes. A 44.4-fold greater UMF solubility in acidic
Tatyana Volkova, Olga Simonova, German Perlovich   +2 more
doaj   +1 more source