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Expression and Purification of Soluble Adenylyl Cyclase from Escherichia coli
2003This chapter outlines methods to purify soluble adenylyl cyclase (AC7) expressed in an Escherichia coli (E. coli) heterologous expression system. Guidelines are provided for constructing the expression plasmids, optimizing expression, culturing, and purifying the proteins. Purification requires two chromatographic steps.
Jeff A, Beeler, Wei-Jen, Tang
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[Nuclear localization region in soluble adenylyl cyclase].
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 2006To locate the region responsible for nuclear localization of protein sAC.The eukaryotic expression vector of vairous sAC deletion mutants were transfected into Hela cells. The localization of each mutant was observed using confocal microscope.For some mutants, the localization of sAC changed.
Qi-ping, Feng +3 more
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Detection of soluble adenylyl cyclase isoforms in plants
Doklady Biochemistry and Biophysics, 2008L A, Lomovatskaya +3 more
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Regulation of cholangiocyte ciliogenesis by soluble adenylyl cyclase
Journal of Hepatology, 2022Hang Lam Li +4 more
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The role of soluble adenylyl cyclase in RAF regulation
Journal of the American Academy of Dermatology, 2014openaire +1 more source