Results 211 to 216 of about 19,255 (216)

[Soluble forms of adenylyl cyclases of spermatozoa].

Tsitologiia, 2015
Soluble (cytosolic) forms of adenylyl cyclases (sAC), the enzymes catalyzing the conversion of ATP to the second messenger cAMP, play a key role in the regulation of spermatogenesis, control maturation of spermatozoa in the epididymis and their capacitation in the female genital tract, which determines their ability to fertilize.
A O, Shpakov, K V, Derkach
openaire   +1 more source

Expression and Purification of Soluble Adenylyl Cyclase from Escherichia coli

2003
This chapter outlines methods to purify soluble adenylyl cyclase (AC7) expressed in an Escherichia coli (E. coli) heterologous expression system. Guidelines are provided for constructing the expression plasmids, optimizing expression, culturing, and purifying the proteins. Purification requires two chromatographic steps.
Jeff A, Beeler, Wei-Jen, Tang
openaire   +2 more sources

[Nuclear localization region in soluble adenylyl cyclase].

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 2006
To locate the region responsible for nuclear localization of protein sAC.The eukaryotic expression vector of vairous sAC deletion mutants were transfected into Hela cells. The localization of each mutant was observed using confocal microscope.For some mutants, the localization of sAC changed.
Qi-ping, Feng, Jin, Zuo, Yan, Meng, Fu-de, Fang   +3 more
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Soluble Adenylyl Cyclase Triggers a Nuclear cAMP Cascade

Science's STKE, 2004
Soluble adenylyl cyclase (sAC) is a recently identified nontransmembrane enzyme that is localized to various subcellular compartments and produces adenosine 3′,5′-monophosphate (cAMP) in response to bicarbonate. Bicarbonate is produced as a by-product of metabolism, and its concentration is altered in response to changes in intracellular ...
openaire   +1 more source

Regulation of cholangiocyte ciliogenesis by soluble adenylyl cyclase

Journal of Hepatology, 2022
Hang Lam Li, Arthur Verhoeven, Simei Go, Jung-Chin Chang, Ronald Oude-Elferink   +4 more
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Detection of soluble adenylyl cyclase isoforms in plants

Doklady Biochemistry and Biophysics, 2008
L A, Lomovatskaya, A S, Romanenko, N V, Filinova, R K, Salyaev   +3 more
openaire   +2 more sources