Results 41 to 50 of about 67,513 (334)

Epigenetic manipulation to improve mouse SCNT embryonic development

Frontiers in Genetics, 2022
Cloned mammals can be achieved through somatic cell nuclear transfer (SCNT), which involves reprogramming of differentiated somatic cells into a totipotent state. However, low cloning efficiency hampers its application severely.
Yamei Li, Yamei Li, Yamei Li, Qiang Sun, Qiang Sun, Qiang Sun   +5 more
doaj   +1 more source

Biotechnological bases of the development of cloned pig embryos

Вавиловский журнал генетики и селекции, 2019
The term ‘clone’ in animal biotechnology refers to an organism derived from non-sexual reproduction, which is both a direct offspring and a genetic copy of the parent organism.
A. V. Lopukhov, G. N. Singina, N. A. Zinovieva   +2 more
doaj   +1 more source

Comparison of in vitro fertilization and nuclear transfer techniques in the production of buffalo pre-implantation embryos

Italian Journal of Animal Science, 2010
This study was conducted to evaluate the timing of cleavage and blastocoel formation of in vitro fertilized and nuclear transfer embryos and to compare the efficiency of in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) techniques in ...
L.C. Cruz, F.V. Mamuad, R.V. de Vera, D.H. Duran, E.P. Atabay, E.C. Atabay   +5 more
doaj   +1 more source

Somatic cell nuclear transfer: failures, successes and the challenges ahead.

International Journal of Developmental Biology, 2019
Somatic cell nuclear transfer (SCNT) has a broad spectrum of potential applications, including rescue of endangered species, production of transgenic animals, drug production, and regenerative medicine.
M. Czernik, D. Anzalone, L. Palazzese, Mami Oikawa, P. Loi   +4 more
semanticscholar   +1 more source

Functional consequences of mitochondrial mismatch in reconstituted embryos and offspring

The Journal of Reproduction and Development, 2019
Animal cloning technology has been developed to produce progenies genetically identical to a given donor cell. However, in nuclear transfer protocols, the recipient oocytes contribute a heritable mitochondrial genomic (mtDNA) background to the progeny ...
Kumiko TAKEDA
doaj   +1 more source

Cloning of a gene-edited macaque monkey by somatic cell nuclear transfer

National Science Review, 2019
Cloning of macaque monkeys by somatic cell nucleus transfer (SCNT) allows the generation of monkeys with uniform genetic backgrounds that are useful for the development of non-human primate models of human diseases.
Zhen Liu, Yijun Cai, Zhaodi Liao, Yuting Xu, Yan Wang, Zhanyang Wang, Xiaoyu Jiang, Yuzhuo Li, Yong Lu, Yanhong Nie, Xiaotong Zhang, Chunyang Li, Xinyan Bian, M. Poo, Hung-Chun Chang, Qiang Sun   +15 more
semanticscholar   +1 more source

Study on Embryo Transfer System for Production of Transgenic Pigs

Journal of Animal Reproduction and Biotechnology, 2015
In the last 10 years, porcine somatic cell nuclear transfer to generate transgenic pig has been performed tremendous development with introduction and knockout of many genes.
Seungwon Na, Euncheol Lee, Ghangyong Kim, Kyuhong Min, Youngkwang Yu, Pantu Kumar Roy, Xun Fang, Bahia Mohamed Salih Hassan, Kiyoung Yoon, Sangtae Shin, Jongki Cho   +10 more
doaj   +1 more source

Interspecies Somatic Cell Nuclear Transfer: Advancements and Problems [PDF]

Cellular Reprogramming, 2013
Embryologists working with livestock species were the pioneers in the field of reprogramming by somatic cell nuclear transfer (SCNT). Without the "Dolly experiment," the field of cellular reprogramming would have been slow and induced plutipotent cells (iPSCs) would not have been conceived.
Lagutina, I., Fulka, H., LAZZARI, GIOVANNA, GALLI, CESARE   +3 more
openaire   +3 more sources

Construction of transgenic swine with induced expression of Cre recombinase

Animal, 2010
Miniature pigs have been recognized as valuable experimental animals in medical research. However, porcine models related to gene knockout of human diseases are not widely available. The objective of this study was to establish Mx1-Cre pigs using somatic
L. Chen, L. Li, D. Pang, Z. Li, T. Wang, M. Zhang, N. Song, S. Yan, L.X. Lai, H. Ouyang   +9 more
doaj   +1 more source

Single cell‐derived clonally expanded mesenchymal progenitor cells from somatic cell nuclear transfer‐derived pluripotent stem cells ameliorate the endometrial function in the uterus of a murine model with Asherman’s syndrome

Cell Proliferation, 2019
Because primary mesenchymal progenitor cells (adult‐MPCs) have various functions that depend on the tissue origin and donor, de novo MPCs from human pluripotent stem cells (hPSCs) would be required in regenerative medicine.
Sung-Min Jun, Mira Park, Ji Yoon Lee, S. Jung, J. Lee, S. Shim, Haengseok Song, Dong Ryul Lee   +7 more
semanticscholar   +1 more source