Results 71 to 80 of about 143,631 (265)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Variables explicatives de la consommation de chauffage dans l’habitat : les leçons de l’analyse d’une copropriété sur données journalières

open access: yesDéveloppement Durable et Territoires, 2017
The aim of this study is to understand which parameters imply the biggest variations of domestic heating consumption. For this purpose, we first gathered real consumption data thanks to the gas meters of ten nearly similar houses in France on two ...
Ghislaine Destais
doaj   +1 more source

Validity of a Wearable Digital Insole for Assessing Gait ON and OFF in Parkinson's Disease

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Gait impairment is a distinctive symptom of Parkinson's disease that negatively impact mobility. We assessed the validity of wearable digital insoles against a validated reference gait analysis system for measuring select gait characteristics in patients with Parkinson's disease. Methods A comparative analysis between digital insoles
Deborah A. Hall   +16 more
wiley   +1 more source

DESIGN AND ANALYSIS OF A SOLAR WATER HEATING SYSTEM WITH THERMAL STORAGE FOR RESIDENTIAL APPLICATIONS [PDF]

open access: yesJournal of Sustainable Energy, 2019
This paper represents a design and analysis of a solar domestic hot water and space heating system with thermal storage for single-family house. To meet the energy demand of residential sector like a house, one of the best options is solar water heating ...
RABBANI R., TARIQ I.
doaj  

Ambition Levels of Nearly Zero Energy Buildings (nZEB) Definitions: An Approach for Cross-Country Comparison

open access: yesBuildings, 2018
Since buildings account for 40% of total energy consumption and 36% of CO2 emissions in the European Union (EU), the directive 2010/31/EU “Energy Performance of Buildings Directive (EPDB)” among other legal provisions concerning the reduction
Juan Fernando Garcia, Lukas Kranzl
doaj   +1 more source

Clinical Outcomes of SEEG‐Guided Radiofrequency Thermocoagulation in Children With Focal Drug‐Resistant Epilepsy: A Multicenter Real‐World Study

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Stereoelectroencephalography‐guided radiofrequency thermocoagulation (SEEG‐RFTC) has emerged as a safe and effective minimally invasive treatment for children with drug‐resistant focal epilepsy. Although evidence from real‐world studies remains limited, numerous pediatric cases have demonstrated promising outcomes. This retrospective
Weitao Chen   +7 more
wiley   +1 more source

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