Results 111 to 120 of about 319,235 (305)
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Single-cell and spatial omics: exploring hypothalamic heterogeneity
Neural Regeneration ResearchElucidating the complex dynamic cellular organization in the hypothalamus is critical for understanding its role in coordinating fundamental body functions.
Muhammad Junaid +2 more
doaj +1 more source
important is access to employment offices in Spain? An urban and non-urban perspective [PDF]
Investigaciones Regionales - Journal of Regional Research, 2011 The aim of this paper is to analyze the effect of the accessibility to employment offices on local unemployment rates according to the distribution of three different types of municipalities: large urban, small urban and non-urban.
Patricia Suárez Cano +2 more
doaj
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Does Context Matter for the Relationship between Deprivation and All-Cause Mortality? The West vs. the Rest of Scotland [PDF]
One of the assumptions that is often made in modeling the relationship between deprivation and mortality is that this relationship will remain the same across space.
Jeremy S. Walker +2 more
core +2 more sources
Overlapping jurisdictions and demand for local public services: does spatial heterogeneity matter? [PDF]
This paper aims to test the existence of vertical interactions in terms of public spending between overlapping local jurisdictions in France using a data set of 110 French municipalities and their corresponding departments in 2001 and 2005.
Matthieu Leprince +2 more
core
Analyzing Spatial Heterogeneity of Housing Prices Using Large Datasets
, 2020 As an obstacle to the hedonic model's reliability, housing submarkets have drawn plenty of scholarly attention because they lack an integrated and standardized classification framework and validation methods.
Wei, Yehua Dennis +2 more
core +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source