Results 181 to 190 of about 16,214,189 (289)
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Advances in Single-Cell and Spatial Omics for Glioblastoma Precision Medicine. [PDF]
Prakash L, Rahman R.
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Spatial Humanities 2024 – Book of Conference Abstracts
We were happy to host the Spatial Humanities 2024 in Bamberg with more than one hundred participants from 26 countries. The conference covered classic spatial humanities topics such as gazetteers and georeferencing, open spatial data, demography and ...
core
Coordinated Regulation of Orchard Variable-Rate Spraying: From Canopy Perception to Actuation-A Review. [PDF]
Dai S +8 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Gray matter alterations and their atlas-based spatial associations with neurotransmitter distributions in isolated REM sleep behavior disorder. [PDF]
Byun JI +6 more
europepmc +1 more source

