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Cancer Cell, 2022
Spatial transcriptomics, with other spatial technologies, has enabled scientists to dissect the organization and interaction of different cell types within the tumor microenvironment. We asked experts to discuss some aspects of this technology from revealing the tumor microenvironment and heterogeneity, to tracking tumor evolution, to guiding tumor ...
Ana C, Anderson +12 more
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Spatial transcriptomics, with other spatial technologies, has enabled scientists to dissect the organization and interaction of different cell types within the tumor microenvironment. We asked experts to discuss some aspects of this technology from revealing the tumor microenvironment and heterogeneity, to tracking tumor evolution, to guiding tumor ...
Ana C, Anderson +12 more
openaire +2 more sources
Expansion Spatial Transcriptomics
Nature Methods, 2022Abstract Capture array-based spatial transcriptomics methods have been widely used to resolve gene expression in diverse tissue contexts, however, their spatial resolution is limited by array density. We present Expansion Spatial Transcriptomics (Ex-ST) to overcome this limitation by clearing and expanding tissue prior to capturing the ...
Yuhang Fan +8 more
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SnapShot: Spatial transcriptomics
Cell, 2022Spatially resolved transcriptomics methodologies using RNA sequencing principles have and will continue to contribute to decode the molecular landscape of tissues. Linking quantitative sequencing data with tissue morphology empowers profiling of cellular morphology and transcription over time and space in health and disease. To view this SnapShot, open
Larsson, Ludvig +4 more
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Spatial transcriptomics and the kidney
Current Opinion in Nephrology & Hypertension, 2022Purpose of review The application of spatial transcriptomics technologies to the interrogation of kidney tissue is a burgeoning effort. These technologies share a common purpose in mapping both the expression of individual molecules and entire transcriptomic signatures of kidney cell types and structures.
Melo Ferreira, Ricardo +2 more
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Spatial Transcriptomics in Kidney Tissue
2023Unlike bulk and single-cell/single-nuclei RNA sequencing methods, spatial transcriptome sequencing (ST-seq) resolves transcriptome expression within the spatial context of intact tissue. This is achieved by integrating histology with RNA sequencing. These methodologies are completed sequentially on the same tissue section placed on a glass slide with ...
Raghubar, Arti M. +9 more
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New adventures in spatial transcriptomics
Developmental Cell, 2022Complex dynamic processes such as development involve the deployment of gene regulatory pathways that transform the spatial arrangement of cells. Disentangling these genetic programs is at the core of many biological problems. Stereo-seq is a promising spatial transcriptomics method, as demonstrated by three papers in this issue of Developmental Cell ...
Maayan, Pour, Itai, Yanai
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Spatially resolved transcriptomics in neuroscience
Nature Methods, 2021One major challenge in neuroscience is to gain a systematic understanding of the extraordinary diversity of brain cell types and how they contribute to brain function. Spatially resolved transcriptomics holds unmatched promise in unraveling the organization of brain cell types and their relationship with connectivity, circuit dynamics, behavior and ...
Jennie L. Close +2 more
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The expanding vistas of spatial transcriptomics
Nature Biotechnology, 2022The formation and maintenance of tissue integrity requires complex, coordinated activities by thousands of genes and their encoded products. Until recently, transcript levels could only be quantified for a few genes in tissues, but advances in DNA sequencing, oligonucleotide synthesis and fluorescence microscopy have enabled the invention of a suite of
Luyi Tian, Fei Chen, Evan Z. Macosko
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Spatial transcriptomics coming of age [PDF]
Nature Reviews Genetics, 2019 Two new spatial transcriptomics techniques published in Nature and Science bring us an important step closer to the goal of achieving transcriptome-wide data at single-cell resolution.
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Spatially resolved transcriptomics and beyond
Nature Reviews Genetics, 2014Considerable progress in sequencing technologies makes it now possible to study the genomic and transcriptomic landscape of single cells. However, to better understand the complexity of multicellular organisms, we must devise ways to perform high-throughput measurements while preserving spatial information about the tissue context or subcellular ...
Crosetto N, Bienko M, van Oudenaarden A
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