Results 101 to 110 of about 185,266 (254)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

A novel germline CDH23 variant as a likely cause of an ultra-giant prolactinoma

Orphanet Journal of Rare Diseases
Giant prolactinomas are defined as pituitary adenomas (PAs) ≥ 4 cm with plasma prolactin level > 1000 ng/ml with no other co-secretory component. The reasons for development of giant prolactinomas are not clear but genetics play an important pathogenic ...
Eman Albasri, Balgees Alghamdi, Avaniyapuram Kannan Murugan, Eyas Othman, Sarah Alotaibi, Mohammad Anas Dababo, Ahmed Alfares, Ali S. Alzahrani   +7 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Large-scale next generation sequencing based analysis of SLCO1B1 pharmacogenetics variants in the Saudi population

Human Genomics
Background SLCO1B1 plays an important role in mediating hepatic clearance of many different drugs including statins, angiotensin-converting enzyme inhibitors, chemotherapeutic agents and antibiotics.
Ewa Goljan, Mohammed Abouelhoda, Asma Tahir, Mohamed ElKalioby, Brian Meyer, Dorota Monies   +5 more
doaj   +1 more source

Correction to: "Remoteness from urban centre does not affect gastric cancer outcomes with established care pathway to specialist centre". [PDF]

Can J Surg, 2023
europepmc   +1 more source

Children with COVID-19 at a specialist centre: initial experience and outcome. [PDF]

Lancet Child Adolesc Health, 2020
Issitt RW, Booth J, Bryant WA, Spiridou A, Taylor AM, du Pré P, Ramnarayan P, Hartley J, Cortina-Borja M, Moshal K, Dunn H, Hemingway H, Sebire NJ.   +12 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

LYSOZYME-INDUCED NEPHROPATHY IN CMML: A RARE BUT SIGNIFICANT COMPLICATION REQUIRING EARLY RECOGNITION AND INTERVENTION

Hematology, Transfusion and Cell Therapy
Background: CMML is a clonal hematopoietic disorder with features of myelodysplasia and myeloproliferation, characterized by monocytosis. Monocytes secrete lysozyme, a cationic protein filtered by the glomerulus and reabsorbed in proximal tubules. Excess
Alfadil Haroon, Mohamed Kindawi, Ahmed S Alotaibi, Ali Debsan Alahmari, Hazzaa Alzahrani, Mansour Alfayez, syed Osman Ahmed   +6 more
doaj   +1 more source