Results 71 to 80 of about 97,963 (258)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Dynamic Analysis of the Cutting Head of a Transverse-Axis Roadheader

Applied Sciences
This study develops a theoretical framework for evaluating the dynamic response and energy performance of the EBH260 transverse-axis roadheader cutting head under horizontal swing cutting conditions.
Xuguang Liu, Shuogui Wang, Yunhao Kang
doaj   +1 more source

Analysis and Research on Working Performance of Shearer Based on Discrete Element Method

IEEE Access, 2019
In view of the complex nonlinear relationship between the shearer mining rate and the cutting specific energy consumption in a fully mechanized mining face, the optimal combination of shearer motion parameters and geometric parameters is studied to ...
Zhizhong Xing, Wei Guo
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Effect of Blade Angles and Thickness on Specific Cutting Energy and Cutting Index of Cassava Stem

Journal of AgriSearch, 2018
Cassava harvesting is highly labour intensive and a partially mechanized operation. Development of a separate cassava stem harvester and incorporation with available cassava digger enhance then harvesting efficiency. To optimize the stem harvester design, this study investigated the effect of blade thickness, approach angle and shear angle on specific ...
BIKRAM JYOTI, K. KARTHIRVEL, CDIVAKAR DURAIRAJ   +2 more
openaire   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Impact of Blade Geometric Parameters on the Specific Cutting Energy of Willow (Salix viminalis) Stems

Applied Sciences
This article presents a model to estimate the specific energy demand for cutting annual willow stems, considering variations in plant moisture content and sliding-cutting angles. The study involved laboratory tests and statistical analyses.
Tomasz Nowakowski, Karol Tucki
doaj   +1 more source

Investigating the Effect of TIG-Assisted Machining on the Specific Cutting Force, Specific Cutting Energy and Chip Shrinkage Coefficient

Transactions of FAMENA
Heat-assisted machining is one of the methods used to process moderate to difficult-to-machine materials. Laser, plasma, induction current, oxy-gas flame, and electric current are the primary energy sources used for preheating. In this paper, the Tungsten Inert Gas (TIG) technique is used, which can be considered an alternative and economical energy ...
Kafkas, Fırat, Uğraş, Atakan
openaire   +3 more sources

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source