Results 91 to 100 of about 1,589,502 (297)
Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins
FEBS Open Bio, EarlyView.We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó +7 more
wiley +1 more source
Bone & Joint ResearchAims: Highly cross-linked polyethylene (HXLPE) greatly reduces wear in total hip arthroplasty, compared to conventional polyethylene (CPE). Cross-linking is commonly achieved by irradiation. This study aimed to compare the degree of cross-linking and in
Peter Wahl +8 more
doaj +1 more source
The CRAPome: a Contaminant Repository for Affinity Purification Mass Spectrometry Data
Nature Methods, 2013 Affinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for the identification of protein-protein interactions. However, for any given protein of interest, determining which of the identified polypeptides represent bona ...
Dattatreya Mellacheruvu +37 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.Epigallocatechin‐3‐gallate (EGCG) acutely inhibited gluconeogenesis and enhanced glycolysis, glycogenolysis, and fatty acid oxidation in perfused rat livers. Mechanistic assays revealed mitochondrial uncoupling, inhibition of pyruvate carboxylation and glucose‐6‐phosphatase, shift of NADH/NAD+ ratios toward oxidation, and loss of membrane integrity ...
Carla Indianara Bonetti +8 more
wiley +1 more source
Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics
FEBS Open Bio, EarlyView.Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini +2 more
wiley +1 more source
Identification of Cryptococcus neoformans by MALDI-TOF mass spectrometry in blood culture
Clinical and Biomedical Research, 2018 This is a report of isolation of Cryptococcus neoformans from blood culture. Identification was conducted by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Max Roberto Batista Araújo +3 more
doaj
Influence of Water on Aging Phenomena of Calendric Stored and Cycled Li-Ion Batteries
Nanoenergy AdvancesWe examine the impact of water (160 ± 41 ppm of reference) on the anode, cathode, separator and electrolyte in two aging scenarios: calendric aging (60 °C, 80 days, charged state), resulting in a triggered current interrupt device (CID), and cycling 1680
Gudrun Wilhelm +7 more
doaj +1 more source
Recommendations for reporting ion mobility Mass Spectrometry measurements
Mass spectrometry reviews (Print), 2018 Here we present a guide to ion mobility mass spectrometry experiments, which covers both linear and nonlinear methods: what is measured, how the measurements are done, and how to report the results, including the uncertainties of mobility and collision ...
V. Gabélica +33 more
semanticscholar +1 more source
Interplay between RNA‐protein interactions and RNA structures in gene regulation
FEBS Open Bio, EarlyView.Methodological advances in mapping transcriptome‐wide RNA‐protein interactions and RNA structures have started to uncover the potential of RNP conformations in gene regulation. Competing RNA–RNA, RNA‐protein and protein–protein interactions shape the compaction and function of RNPs throughout their lifetime and may provide novel therapeutic targets in ...
Jenni Rapakko +2 more
wiley +1 more source