Results 91 to 100 of about 2,742,251 (343)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Kinetic selectivity in metal-organic framework chemical sensors

Nature Communications
Selective detection of specific volatile organic compounds (VOCs) is crucial for health, safety, and environmental applications, but current sensors suffer from poor selectivity and struggle to measure specific VOCs in the presence of interfering ...
Aleksander Matavž, Margot F. K. Verstreken, Leen Boullart, Max L. Tietze, Masaya Sugihara, Lars Heinke, Rob Ameloot   +6 more
doaj   +1 more source

Comparison of 3 Tesla proton MR spectroscopy, MR perfusion and MR diffusion for distinguishing glioma recurrence from posttreatment effects [PDF]

, 2011
James R. Fink, Robert B. Carr, Eiji Matsusue, Ramesh S. Iyer, Jason K. Rockhill, David R. Haynor, Kenneth R. Maravilla   +6 more
openalex   +1 more source

Prediction of Na- and Ca-montmorillonite contents and swelling properties of clay mixtures using Vis-NIR spectroscopy

, 2022
Yujin Byun, Chanyoung Seo, Taehyun Yun, Yongsung Joo, Ho Young Jo   +4 more
openalex   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

LDA+DMFT approach to core-level spectroscopy: Application to 3d transition metal compounds [PDF]

, 2017
Atsushi Hariki, Takayuki Uozumi, J. Kuneš   +2 more
openalex   +1 more source

Characterization of Hyaluronic Acid-Coated PLGA Nanoparticles by Surface-Enhanced Raman Spectroscopy [PDF]

, 2022
Giuseppe La Verde, Antonio Sasso, Giulia Rusciano, Angela Capaccio, Sabato Fusco, Laura Mayol, Marco Biondi, Teresa Silvestri, Paolo A. Netti, M. La Commara, Valeria Panzetta, Mariagabriella Pugliese   +11 more
openalex   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source