Results 101 to 110 of about 1,116,535 (288)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Parametric Optimization for High Speed FLIM Implementation

open access: yesMATEC Web of Conferences, 2015
FLIM (Fluorescence Lifetime Imaging Microscopy) has been considered an effective technique to investigate chemical properties of the specimens, especially of biological samples.
Kim Jayul, Ryu Jiheun, Gweon Daegab
doaj   +1 more source

An Aggregated Optimization Model for Multi-Head SMD Placements [PDF]

open access: yes
In this article we propose an aggregate optimization approach by formulating the multi-head SMD placement optimization problem into a mixed integer program (MIP) with the variables based on batches of components.
Ashayeri, J., Ma, N., Sotirov, R.
core   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Mission Optimized Speed Control

open access: yes, 2017
Transportation underlines the vehicle industry's critical role in a country's economic future.The amount of goods moved, specically by trucks, is only expected to increase inthe near future. This work attempts to tackle the problem of optimizing fuel consumptionin Volvo trucks, when there are hard constraints on the delivery time and speed limits ...
He, Jincan, Bhatt, Sundhanva
openaire   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

WEB APPLICATIONS READABILITY [PDF]

open access: yes
The emergence known by online press companies requires written filtered information for a better understanding and speed the understanding of texts and messages that are posted. Testing the readability of text in an online environment is important in the
Eugen-Gabriel Garais
core  

Optimizing the linearity in high-speed photodiodes

open access: yesOptics Express, 2018
Analog photonic links require high-fidelity, high-speed optical-to-electrical conversion for applications such as radio-over-fiber, synchronization at kilometer-scale facilities, and low-noise electronic signal generation. Photodetector nonlinearity is a particularly vexing problem, causing signal distortion and excess noise, especially in systems ...
J. Davila-Rodriguez   +9 more
openaire   +3 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

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