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Sperm DNA fragmentation testing: Summary evidence and clinical practice recommendations [PDF]
Andrologia, 2021 We herein summarise the evidence concerning the impact of sperm DNA fragmentation in various clinical infertility scenarios and the advances on sperm DNA fragmentation tests. The collected evidence was used to formulate 41 recommendations.
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DNA fragmentation of sperm in automobile painters
Toxicology and Industrial Health, 2021Reproductive health is a major concern for workers constantly exposed to chemicals at work, especially in developing countries. The problems mainly stemmed from chronic exposure to the chemicals contained in paint thinner, which is mainly toluene, used during automobile painting. Unfortunately, in Indonesia, there is no study on the reproductive health
Dicky Faizal Irnandi +2 more
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Seminars in Reproductive Medicine, 2021
AbstractSperm DNA fragmentation refers to the accumulation of adducts as well as single- or double-strand DNA breaks and reflects the sperm DNA quality. Current data suggest that there are differences in sperm DNA quality among individuals with high or low fertility, and this observation has led to the idea that testing sperm DNA fragmentation could be
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AbstractSperm DNA fragmentation refers to the accumulation of adducts as well as single- or double-strand DNA breaks and reflects the sperm DNA quality. Current data suggest that there are differences in sperm DNA quality among individuals with high or low fertility, and this observation has led to the idea that testing sperm DNA fragmentation could be
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Sperm DNA fragmentation: causes and identification
Zygote, 2019SummarySperm DNA fragmentation is referred to as one of the main causes of male infertility. Failures in the protamination process, apoptosis and action of reactive oxygen species (ROS) are considered the most important causes of DNA fragmentation.
Thais Rose dos Santos Hamilton +1 more
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Sperm DNA Fragmentation: Consequences for Reproduction
2019DNA fragmentation, or the accumulation of single- and double-strand DNA breaks, is a common property of sperm, and an increase in the level of sperm DNA fragmentation is known to influence natural reproduction. The effect of sperm DNA fragmentation on male infertility and assisted reproductive treatment (ART) outcomes remains controversial and is one ...
Luke, Simon +2 more
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Sperm DNA Fragmentation and Fertility
Elevated levels of sperm deoxyribonucleic acid (DNA) fragmentation (SDF) have been associated with several adverse reproductive outcomes, including: lower natural and assisted reproductive technology (ART) pregnancy rates, abnormal embryo development, and recurrent pregnancy loss.Jessica, Marinaro, Peter N, Schlegel
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Sperm DNA Fragmentation: Mechanisms of Origin
2019Spermatozoa have the task to deliver an intact paternal genome to the oocyte and to support a successful embryo development. The high levels of sperm DNA fragmentation (sDF) found in sub-/infertile men threat human reproduction and health of the offspring.
Muratori, Monica +3 more
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Sperm DNA fragmentation is related to sperm morphological staining patterns
Reproductive BioMedicine Online, 2015In this prospective comparative study, sperm DNA fragmentation (sDNAfrag) was compared at each step of a sequential semen preparation, with semen parameters according to their degree of severity. At each step (fractions) of the sequential procedure, sDNAfrag was determined: fresh (Raw), after gradient centrifugation, washing, and swim-up (SU) for 70 ...
Rosália, Sá +4 more
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Fragmentation of sperm DNA using the TUNEL method
Actas Urológicas Españolas (English Edition), 2014To establish the validity of the TUNEL assay in determining sperm DNA fragmentation, the relationship between the degree of fragmentation and the seminal parameters and the sample needed to conduct the test.We used semen samples from healthy fertile men (n=33), patients who consulted for infertility with a prescription for the TUNEL assay (n=77) and ...
P H, Chenlo +9 more
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Assessing Sperm DNA Fragmentation with the Sperm Chromatin Dispersion Test
2010The sperm cell has evolved to transmit a paternal haploid genome to the oocyte and form a new embryo. Therefore, it is essential that the integrity of this genome be evaluated as part of the standard semen analysis. The assessment of DNA fragmentation is consequently considered as an important parameter of sperm quality. The Sperm Chromatin Dispersion (
José Luis, Fernández +3 more
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