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Mechanics of the spindle apparatus

Seminars in Cell & Developmental Biology, 2020
During mitosis microtubules self-organize to form a bipolar mitotic spindle structure, which positions the sister chromatids on the spindle mid-plane and separates them afterwards. Previous studies have identified many spindle associated proteins.
Stefanie Redemann, Ehssan Nazockdast
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MTA1 localizes to the mitotic spindle apparatus and interacts with TPR in spindle assembly checkpoint regulation

Biochemical and Biophysical Research Communications, 2023
We previously identified a cell cycle-dependent periodic subcellular distribution of cancer metastasis-associated antigen 1 (MTA1) and unraveled a novel role of MTA1 in inhibiting spindle damage-induced spindle assembly checkpoint (SAC) activation in cancer cells.
Jian Liu   +6 more
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A gene required for the separation of chromosomes on the spindle apparatus in yeast

Cell, 1986
We describe the phenotypes caused by a cold-sensitive lethal mutation (ndc1-1) that defines the NDC1 gene of yeast. Incubation of ndc1-1 at a nonpermissive temperature causes failure of chromosome separation in mitosis but does not block the cell cycle.
J. H. Thomas, David Botstein
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Staining of the spindle apparatus in human lymphocyte and fibroblast cultures

Human Genetics, 1976
The spindle apparatus of human mitoses can be preferentially stained with Giemsa if the cells are fixed with methanol--acetic acid and treated for about 15 min with 0.1 X SSC at 92 degrees C and for 18 h with 2 X SSC at 60 degrees C.
K. Sperling, F. Pera
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THE MITOTIC SPINDLE APPARATUS OFNEUROSPORA CRASSA

Canadian Journal of Genetics and Cytology, 1971
Electron microscopic studies of the sl-327 mutant of Neurospora crassa reveal that somatic nuclear division in hyphlet somatic cells involves a definite spindle apparatus consisting of spindle microtubules terminating at specific loci on the external surface of the nuclear envelope. These loci, termed spindle plaques, have been observed in a variety of
R. P. Wagner   +2 more
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Cytokinesis: relative alignment of the cell division apparatus and the mitotic spindle

Current Opinion in Cell Biology, 2003
The cell division apparatus is assembled at different stages of the cell cycle in different eukaryotic organisms. Mechanisms exist in all organisms, however, to ensure that the cell division apparatus and the mitotic spindle are aligned perpendicular to each other.
Wang, Hongyan   +2 more
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Reactivation of isolated mitotic apparatus: Metaphase versus anaphase spindles

Cell Motility, 1991
AbstractMitotic spindles isolated from sea urchin eggs can be reactivated to undergo mitotic processes in vitro. Spindles incubated in reactivation media containing sea urchin tubulin and nucleotides undergo pole‐pole elongation similar to that observed in living cells during anaphase‐B.
Robert E. Palazzo   +2 more
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Arrangement of spindle apparatus in mitoses of different ploidy

Experimental Cell Research, 1975
Abstract In non-hypotonically treated mitoses from tissue cultures of Microtus agrestis , both the constitutive heterochromatin of the sex chromosomes and the spindle apparatus were stained by the Giemsa C-banding technique. By means of counting the heterochromatic chromosomes, we determined the cell ploidy and studied the number of centrioles and ...
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