Results 41 to 50 of about 4,913,708 (237)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Experimental Study on Active Thermal Protection for Electronic Devices Used in Deep−Downhole−Environment Exploration

Energies, 2023
Electronic devices are commonly used for exploiting and extracting shale oil in deep downhole environments. However, high−temperature−and−pressure downhole environments jeopardize the safe operation of electronic components due to their severe thermal ...
Shihong Ma, Shuo Zhang, Jian Wu, Yongmin Zhang, Wenxiao Chu, Qiuwang Wang   +5 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Generation of Optical Vortices With Polarization-Insensitive Metasurfaces

IEEE Photonics Journal, 2020
Traditionally, optical vortices (OVs) were generated with diffractive optical elements (DOEs) such as spiral phase plate (SPP), fork grating, spatial light modulator (SLM), and liquid crystal display (LCD).
Z. Shen, R. Li, Y. Z. Xue, Z. Y. Qiu, Z. Y. Xiang, J. Y. Zhou, B. F. Zhang   +6 more
doaj   +1 more source

Calibration of the Soleil–Babinet Compensator Based on the Vectorial Optical Field

Photonics, 2022
The Soleil–Babinet compensator (SBC) is a variable retarder and has been used in a variety of application fields. A scheme based on the vectorial optical field is proposed to calibrate the SBC by transforming the change of the phase retardation into the ...
Yayun Ma, Fen Yang, Dong’e Zhao
doaj   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Propagation of an Azimuthally Polarized Terahertz Laser Beams with a Phase Singularity

East European Journal of Physics
Analytical expressions are derived to describe the nonparaxial diffraction of modes in a dielectric waveguide resonator for a terahertz laser. The study examines the interaction between azimuthally polarized TE0m (m = 1, 2, 3) modes and a spiral phase ...
Andrey V. Degtyarev, Mykola M. Dubinin, Vyacheslav A. Maslov, Konstantin I. Muntean, Vladislav S. Senyuta   +4 more
doaj   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Numerical Simulation of Performance Improvement of Underwater Lidar by Using a Spiral Phase Plate as Spatial Filter

IEEE Photonics Journal, 2022
In turbid water, target reflected lidar signal is easily buried in “clutter” due to scattering. We proposed an underwater laser ranging model applying an optical vortex in the receiver to separate the scattering clutter from the target ...
YingQi Liao, SuHui Yang, Kun Li, Yan Hao, Zhuo Li, Xin Wang, JinYing Zhang   +6 more
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source