Results 151 to 160 of about 13,950 (190)

RNAi Screening in Spodoptera frugiperda

2016
RNA interference is a potent and precise reverse genetic approach to carryout large-scale functional genomic studies in a given organism. During the past decade, RNAi has also emerged as an important investigative tool to understand the process of viral pathogenesis.
Subhanita, Ghosh, Gatikrushna, Singh, Bindiya, Sachdev, Ajit, Kumar, Pawan, Malhotra, Sunil K, Mukherjee, Raj K, Bhatnagar   +6 more
openaire   +2 more sources

Flight performance and metabolism of the moth Spodoptera frugiperda

Journal of Insect Physiology, 1971
Abstract Tethered moths were flown continuously for 4 to 7 hr in a sealed flight mill which allowed the trapping of CO2 and the recording of distance flown. The respiratory rate was approximately 10 cal/km per g or 30 cal/hr per g in flight, and 5 cal/hr per g at rest.
J K, Nayar, E, Van Handel
openaire   +2 more sources

Characterization of the 20S proteasome of the lepidopteran, Spodoptera frugiperda

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2019
Multiple complexes of 20S proteasomes with accessory factors play an essential role in proteolysis in eukaryotic cells. In this report, several forms of 20S proteasomes from extracts of Spodoptera frugiperda (Sf9) cells were separated using electrophoresis in a native polyacrylamide gel and examined for proteolytic activity in the gel and by Western ...
Oksana I, Kravchuk, Yulia V, Lyupina, Pavel A, Erokhov, Alexander D, Finoshin, Kim I, Adameyko, Maryia Yu, Mishyna, Andrey V, Moiseenko, Olga S, Sokolova, Olga V, Orlova, Svetlana N, Beljelarskaya, Marina V, Serebryakova, Maria I, Indeykina, Anna E, Bugrova, Alexey S, Kononikhin, Victor S, Mikhailov   +14 more
openaire   +2 more sources

Genome-wide analysis of G-quadruplex in Spodoptera frugiperda

International Journal of Biological Macromolecules, 2023
Spodoptera frugiperda (Lepidoptera: Noctuidae) is a globally distributed lepidopteran crop pest that has developed resistance to most insecticides. The G-quadruplex (G4) is a secondary structure in the genome enriched in the promoters for regulating gene expression. However, little is known about G4 in S.
Zhongyuan, Deng, Yudong, Ren, Lina, Guo, Xingcheng, Xie, Lixiang, Wang, Xianchun, Li   +5 more
openaire   +2 more sources

Pheromonal Divergence Between Two Strains of Spodoptera frugiperda

Journal of Chemical Ecology, 2013
Spodoptera frugiperda consists of two genetically and behaviorally different strains, the corn- and the rice-strain, which seem to be in the process of sympatric speciation. We investigated the role of strain-specific sexual communication as a prezygotic mating barrier between both strains by analyzing strain-specific variation in female pheromone ...
Unbehend, M., Hänniger, S., Meagher, R.L., Heckel, D.G., Groot, A.T.   +4 more
openaire   +5 more sources

Expression and purification of recombinant nattokinase in Spodoptera frugiperda cells

Biotechnology Letters, 2007
A recombinant baculovirus, rv-egfp-NK, containing a reporter gene encoding the enhanced green fluorescent protein (EGFP), was used to express nattokinase (NK), a fibrinolytic enzyme, in Spodoptera frugiperda (SF-9) cells. The recombinant protein also included a histidine tag for purification using Ni(2+) resins. The recombinant NK, approximately 30 kDa,
Xiaoxiang, Li, Xiaoli, Wang, Shaoling, Xiong, Jing, Zhang, Litao, Cai, Yanyan, Yang   +5 more
openaire   +2 more sources

The evaluation of the biological control of Spodoptera Frugiperda in corn cultivation: biological control of Spodoptera Frugiperda

2018
Corn is important grain in human nutrition and raw material for the elaboration of balanced food products. It has been world-wide reported that the most aggressive plague insect is the cogollero worm (Spodoptera frugiperda) since it is considered a defoliator, consumes foliage and attack buds, causing gouging on the softest parts of leaves, slices ...
Ezeta León, Jorge, García Brito, Oswaldo, Gordillo Manssur, Fabian   +2 more
openaire   +1 more source

Production of rotavirus-like particles in Spodoptera frugiperda larvae

Journal of Virological Methods, 2008
A strategy for the production of virus-like particles (VLPs) from simian rotavirus in larvae of the lepidopteran Spodoptera frugiperda is described. VP2 and VP6 coding sequences were co-expressed in larvae co-infected with recombinant baculovirus and these structural proteins self-assembled into VLPs that were secreted and accumulated in the haemolymph.
P, Molinari, A, Peralta, O, Taboga
openaire   +2 more sources

AN OVERVIEW ON THE LIFE CYCLE OF SPODOPTERA FRUGIPERDA

SARPS: Journal of Advanced Research in Plant Science
The fall armyworm (FAW), or Spodoptera frugiperda, (J.E. Smith, 1797) is a significant pest affecting global maize production and other economically important crops. It is an invasive and extremely damaging insect that affects maize and other crops all over the world. S.
Puja Dewanda, Garima Kumawat
openaire   +1 more source

Purification of DNA for the transfection of a Spodoptera frugiperda cell line

Methods in Cell Science, 2002
Spodoptera frugiperda (Sf-9) cells have been widely used in baculovirus expression systems, transient gene expression studies and transgenic cell lines. These applications commonly require the transfection of bacterial plasmid DNA. One of the most reliable methods of preparing transfection-quality plasmid DNA is cesium chloride (CsCl) density gradient ...
Jeffrey M, Slack, Susan D, Lawrence
openaire   +2 more sources