Results 41 to 50 of about 353,966 (351)

A rapid method for Candida DNA preparation from sputum, and its clinical application

Journal of Infection in Developing Countries
Introduction: The analysis and identification of Candida in sputum specimens is of significance in clinical settings. The aim of this study was to assess the feasibility of a previously validated rapid method for preparing Candida DNA in conjunction ...
Qingqing Sun, Yun Xie, Hanbing Meng, Lina Zhang, Zhengxin He   +4 more
doaj   +1 more source

The 10–23 DNAzyme in Biosensing and Diagnostics: Applications, Challenges, and Future Directions

Angewandte Chemie, EarlyView.
This review focuses on the 10–23 DNAzyme in diagnostics, spanning unregulated and regulated target‐recognition modes, and their functionalization across colorimetric, fluorescent, electrochemical, electrochemiluminescent, and intracellular biosensing strategies.
Connor Nurmi, Jake Brill, Sanne Roumans, Amal Mathai, John D. Brennan, Yingfu Li   +5 more
wiley   +2 more sources

The Role of TB-LAMP Method in Detecting Mycobacterium tuberculosis from Sputum of Patients Suspected of Having Pulmonary Tuberculosis

Acta Medica Indonesiana, 2020
Background: Indonesia is one of the countries with the highest incidence of tuberculosis (TB) in the world. Appropriate diagnosis is an effort to control TB. The World Health Organization has recommended loop-mediated isothermal amplification (LAMP). In
Mardiastuti H Wahid, Agus Sjahrurachman, Ardath H Sitorus, Erlina Burhan   +3 more
doaj  

On the analysis of tuberculosis studies with intermittent missing sputum data [PDF]

, 2015
In randomized studies evaluating treatments for tuberculosis (TB), individuals are scheduled to be routinely evaluated for the presence of TB using sputum cultures. One important endpoint in such studies is the time of culture conversion, the first visit
Abraham, Maria, Chaisson, Richard, Geiter, Lawrence, McDermott, Aidan, Rotnitzky, Andrea Gloria, Scharfstein, Daniel   +5 more
core   +2 more sources

Direct Whole-Genome Sequencing of Sputum Accurately Identifies Drug-Resistant Mycobacterium tuberculosis Faster than MGIT Culture Sequencing

Journal of Clinical Microbiology, 2018
The current methods available to diagnose antimicrobial-resistant Mycobacterium tuberculosis infections require a positive culture or only test a limited number of resistance-associated mutations.
R. Doyle, Carrie Burgess, Rachel J. Williams, R. Gorton, H. Booth, James Brown, J. Bryant, Jacqueline Chan, D. Creer, J. Holdstock, H. Kunst, S. Lozewicz, G. Platt, E. Y. Romero, G. Speight, Simon Tiberi, I. Abubakar, M. Lipman, Timothy D McHugh, J. Breuer   +19 more
semanticscholar   +1 more source

Tailoring asthma treatment on eosinophilic markers (exhaled nitric oxide or sputum eosinophils): a systematic review and meta-analysis

Thorax, 2018
Background Asthma guidelines guide health practitioners to adjust treatments to the minimum level required for asthma control. As many people with asthma have an eosinophilic endotype, tailoring asthma medications based on airway eosinophilic levels ...
H. Petsky, C. Cates, Kayleigh Kew, A. Chang   +3 more
semanticscholar   +1 more source

Sputum exosomes: promising biomarkers for idiopathic pulmonary fibrosis

Thorax, 2018
Idiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease of unknown aetiology which leads rapidly to death. As diagnosis of IPF is complex, we aimed to characterise microRNA (miRNA) content of exosomes from sputum of ...
Makon-Sébastien NJOCK, J. Guiot, M. Henket, Olivier Nivelles, M. Thiry, F. Dequiedt, J. Corhay, R. Louis, I. Struman   +8 more
semanticscholar   +1 more source

Metabolic strategies that enable oral commensal persistence in a lower airway environment

mBio
Oral microbiota are increasingly implicated in chronic inflammatory diseases beyond the mouth, including bronchiectasis, a condition marked by persistent airway inflammation, mucus accumulation, and limited therapeutic options.
Ashley M. Toney, Junu Koirala, Apollo Stacy   +2 more
doaj   +1 more source

Rapid method for Mycobacterium tuberculosis identification using electrospray ionization tandem mass spectrometry analysis of mycolic acids [PDF]

, 2013
Mycolic acids (MAs), which play a crucial role in the architecture of mycobacterial cell walls, were analyzed using electrospray ionization tandem mass spectrometry. A targeted analysis based on the 10 most abundant and characteristic multiple reaction
Beata Janiszewska-Drobinska, Behr, Beukes, Brennan, Butler, Centers for Disease Control & Prevention (CDC), Chang, Daley, Deng, Dorman, Drobniewski, El Khéchine, Fujiwara, Jafari, Konrad Kowalski, Kowalski, Lawn, Lotz, Magdalena Druszczyńska, Ottenhoff, Park, Rafał Szewczyk, Saleeb, Schluger, Shitikov, Shui, Shui, Siddiqi, Song, Steingart, Takayama, Toney, Tostmann, Viader-Salvadó, Vittor, Wang, World Health Organization (WHO), Yassin, Yuan   +38 more
core   +1 more source

The CF-Sputum Induction Trial (CF-SpIT) to assess lower airway bacterial sampling in young children with cystic fibrosis: a prospective internally controlled interventional trial

The Lancet Respiratory Medicine, 2018
Summary Background Pathogen surveillance is challenging but crucial in children with cystic fibrosis—who are often non-productive of sputum even if actively coughing—because infection and lung disease begin early in life.
K. Ronchetti, J. Tame, C. Paisey, L. Thia, I. Doull, R. Howe, E. Mahenthiralingam, J. Forton   +7 more
semanticscholar   +1 more source