Results 91 to 100 of about 66,303 (280)

Environment Determines Epistatic Patterns for a ssDNA Virus [PDF]

Genetics, 2014
Abstract Despite the accumulation of substantial quantities of information about epistatic interactions among both deleterious and beneficial mutations in a wide array of experimental systems, neither consistent patterns nor causal explanations for these interactions have yet emerged.
S Brian, Caudle, Craig R, Miller, Darin R, Rokyta   +2 more
openaire   +2 more sources

Systematic Position Mapping of Split CRISPR‐Cas12a Activators Enables Highly Sensitive miRNA Detection and Cancer Cell Stratification

Advanced Science, EarlyView.
ABSTRACT Amplification‐free Cas12a diagnostics with split crRNA enable rapid and programmable target recognition, yet insufficient understanding of DNA activator architecture prevents predictable control over trans‐cleavage activity and sensitivity. Here we systematically map over 200 split DNA activator configurations by introducing nicks at every ...
Xiaoyan Tang, Zhe Li, Yuning Lu, Miao Ma, Zijian Mo, Jiajun Ke, Xinyu Luan, Tiangang Luan, Junqiu Zhai   +8 more
wiley   +1 more source

Senkyunolide I Inhibits mtDNA‐cGAS‐STING Signaling in Macrophages via Targeting VDAC1 Oligomerization to Attenuate Ulcerative Colitis

Advanced Science, EarlyView.
In macrophages, senkyunolide I (SEI) directly targets the K12 residue of VDAC1 to inhibit its stress‐induced oligomerization, a critical upstream event that effectively prevents mitochondrial DNA release and subsequent cGAS‐STING pathway activation.
Zhiming Ye, Yihang Huang, Bohao Han, Lei Zhang, Can Yu, Yang Yang, Bing Liu, Yongping Jian, Zhixiang Xu, Cheng Zeng   +9 more
wiley   +1 more source

Alternating Shear Force of Respiration Regulates Cell Interactions of Fibroblasts and Macrophages to Promote Soft Tissue Integration of Chest Wall Polyetheretherketone Implants

Advanced Science, EarlyView.
Given that PEEK chest wall implants induce shear force at the soft tissue‐implant interface due to respiratory motion in the early in vivo post‐implantation stage. We confirmed that this mechanical stimulus triggers the PIEZO1/ITGB1/YAP1 signalling axis in L929 and the STAT6/p‐STAT6 pathway in RAW264.7, which in turn mediates adhesion and migration of ...
Rou Huang, Yangfan Huo, Jin Liu, Sida Liu, Minghai Ma, Hao Guo, Yuanquan Zhang, Xing Li, Xiao Liang, Jiawei Xiu, Ying Sun, Lijun Huang, Xiaolong Yan, Weiwei Wu, Lei Wang   +14 more
wiley   +1 more source

Structural Basis of Homology-Directed DNA Repair Mediated by RAD52

iScience, 2018
Summary: RAD52 mediates homologous recombination by annealing cDNA strands. However, the detailed mechanism of DNA annealing promoted by RAD52 has remained elusive.
Mika Saotome, Kengo Saito, Takeshi Yasuda, Hideaki Ohtomo, Shusei Sugiyama, Yoshifumi Nishimura, Hitoshi Kurumizaka, Wataru Kagawa   +7 more
doaj   +1 more source

Elasticity of strongly stretched ssDNA [PDF]

Physica A: Statistical Mechanics and its Applications, 2007
We present a simple model which describes elastic response of single-stranded DNA (ssDNA) to stretching, including the regime of very high force (up to 1000 pN). ssDNA is modelled as a discreet persistent chain, whose ground state is a zigzag rather than a straight line configuration.
openaire   +2 more sources

Controllable Cascade Aggregation Ion Programmed Nanomachines and Simple Isolation Enable Tumor‐Derived Small Extracellular Vesicles Detection for Enhanced Breast Cancer Assessment

Advanced Science, EarlyView.
This study developed a convenient, enzyme‐free detection platform based on controllable aggregation ion nanomachines. Its favorable anti‐interference capacity and high sensitivity meet the detection requirements for clinical samples processed via a simple filtration method within 10 min.
Zhihao Zhang, Zijing Liu, Xiangyue Meng, Pengjun Jiang, Jie Qu, Yu Jiang, Binxu Qiu, Jie Chen, Piaopiao Chen   +8 more
wiley   +1 more source

Extracting ssDNA from dsDNA molecules using probe strands.

, 2023
(a) The DNA template molecule with two nicks at sites A and B. After applying gentle heat, the ssDNA between the two nicks is selectively denatured to create a toehold at domain 0.
Arnav Solanki (14066385), Zak Griffin (17240651), Purab Ranjan Sutradhar (17240654), Caiden Merritt (17240660), Amlan Ganguly (13362855), Marc Riedel (215256), Karisha Pradhan (17240657)   +6 more
core   +1 more source

Entropy‐Driven Physical Amplification in Multivalent Biosensing

Advanced Science, EarlyView.
Detecting scarce molecules usually demands enzymatic amplification such as PCR. A statistical‐mechanical theory now reveals a purely physical alternative: distributing a fixed binding strength over more linker arms exponentially lowers the concentration at which multivalent sensors switch on.
Yuhan Peng, Xiuyang Xia, Ran Ni
wiley   +1 more source

The smallest ssDNA phage infecting a marine bacterium

, 2019
In the marine environment, only a few lytic single-stranded DNA (ssDNA) phages have been isolated and characterized, despite the fact that diverse ssDNA bacteriophages have been discovered via metagenomic studies.
Zhan, Yuanchao, Yuanchao Zhan, Feng Chen, Chen, Feng   +3 more
core   +1 more source