Results 81 to 90 of about 66,303 (280)
Nature Communications, 2023 Accumulation of single stranded DNA (ssDNA) gaps in the nascent strand during DNA replication has been associated with cytotoxicity and hypersensitivity to genotoxic stress, particularly upon inactivation of the BRCA tumor suppressor pathway.
Anastasia Hale +4 more
doaj +1 more source
Advanced Science, EarlyView.Anti‐PD‐1/PD‐L1 blockade has revolutionized cancer immunotherapy, but is ineffective against endocrine‐treated (i.e., Tamoxifen), relapsed ER+ breast cancer (BC) patients. This study provides insight into the sub‐optimal response of ER+BCs to anti‐PD‐1/PD‐L1 blockade – highlighting the induction of STING and the CEACAM1/TIM3 axis after chronic ...
Marvin Angelo E Aberin +20 more
wiley +1 more source
Upgrading SELEX Technology by Using Lambda Exonuclease Digestion for Single-Stranded DNA Generation
Molecules, 2009 The generation of single-stranded DNA (ssDNA) molecules plays a key role in the SELEX (Systematic Evolution of Ligands by EXponential enrichment) combinatorial chemistry process and numerous molecular biology techniques and applications, such as DNA ...
Meltem Avci-Adali +4 more
doaj +1 more source
Role of Saccharomyces single-stranded DNA-binding protein RPA in the strand invasion step of double-strand break repair. [PDF]
PLoS Biology, 2004 The single-stranded DNA (ssDNA)-binding protein replication protein A (RPA) is essential for both DNA replication and recombination. Chromatin immunoprecipitation techniques were used to visualize the kinetics and extent of RPA binding following ...
Xuan Wang, James E Haber
doaj +1 more source
, 2018 (A) Schematic representation of the DNA/RNA products generated by Endo V. (B) The nuclease activities of TREX1 in digesting bubbled DNAs containing a hypoxanthine base (also named dI), including dI-bubbled DNA and dI-bubbled DNA with 5′-overhang.
Ruei-Yue Liang (5180999) +6 more
core +1 more source
Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone
Advanced Science, EarlyView.A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad +12 more
wiley +1 more source
Recombination in Eukaryotic Single Stranded DNA Viruses
Viruses, 2011 Although single stranded (ss) DNA viruses that infect humans and their domesticated animals do not generally cause major diseases, the arthropod borne ssDNA viruses of plants do, and as a result seriously constrain food production in most temperate ...
Philippe Roumagnac +5 more
doaj +1 more source
, 2019 ssDNA Extraction Protocol This protocol is based on the Oligonucleotide extraction protocol of the NEB PCR & Clean-up Kit and the Qiagen DNeasy Protocol with a few modifications to maximize DNA output. First results of this protocol will be published soon, including first sequencing results of the so extracted DNA.
openaire +1 more source
The ssDNA binding pockets and wrapped ssDNA binding model.
, 2014 Top: DelPhi electrostatic surface ranging from -3 (deep red) to 3 (deep blue). Zn is colored gray, and was not used in the electrostatic potential calculations.
Yuh-Ju Sun (151128) +7 more
core +1 more source
Smart Nanotechnologies for Multimodal Neuromodulation and Brain Interfacing
Advanced Science, EarlyView.Recent advances in smart nanotechnologies are expanding the toolbox for brain interfacing, from wireless neuromodulation and high‐resolution sensing to targeted delivery within the central nervous system. By combining responsive nanomaterials with bioinspired design, these platforms enable multimodal interactions with neurons and glia, while also ...
Tommaso Curiale +6 more
wiley +1 more source