Results 101 to 110 of about 66,303 (280)

Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo

Advanced Science, EarlyView.
As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Qingquan Xiao, Zhijin Tian, Luqi Weng, Kun Xu, Dingyi Han, Zhaowei Wu, Junnan Li, Shangpu Li, Qijing Zhang, Xiaohui Zhao, Guoling Li, Yinghui Wei, Zhen Gu   +12 more
wiley   +1 more source

Early Infection for Mass Production of Artificial Single-Stranded DNA with Escherichia coli

Applied Sciences
Large quantities of artificial single-stranded DNA (ssDNA) with user-defined sequences are increasingly required to exploit the potential of DNA nanotechnology.
Nathalie Hafner, Nazanin Nematzadeh Somehsaraei, Maximilian N. Honemann, Hendrik Dietz, Dirk Weuster-Botz   +4 more
doaj   +1 more source

Quantitative Viral Community DNA Analysis Reveals the Dominance of Single-Stranded DNA Viruses in Offshore Upper Bathyal Sediment from Tohoku, Japan

Frontiers in Microbiology, 2018
Previous studies on marine environmental virology have primarily focused on double-stranded DNA (dsDNA) viruses; however, it has recently been suggested that single-stranded DNA (ssDNA) viruses are more abundant in marine ecosystems.
Mitsuhiro Yoshida, Tomohiro Mochizuki, Tomohiro Mochizuki, Syun-Ichi Urayama, Yukari Yoshida-Takashima, Shinro Nishi, Miho Hirai, Hidetaka Nomaki, Hidetaka Nomaki, Yoshihiro Takaki, Takuro Nunoura, Ken Takai   +11 more
doaj   +1 more source

Rapid Exonuclease‐Based De Novo Sequencing Strategy for Long Oligonucleotides via Liquid‐Phase Ion Trap Coupled With Mass Spectrometry

Advanced Science, EarlyView.
A liquid‐phase ion trap (LPIT) coupled with ESI‐MS enables rapid, high‐fidelity de novo sequencing of long oligonucleotides in ∼30 min. By applying an electric field to dissociate metal adducts in solution, LPIT delivers clean spectra, superior mass accuracy, and full sequence coverage, surpassing MALDI‐TOF and LC‐MS.
Song Lu, Jie Hong, Xinyan Fu, Di Wang, Yunhua Gao, Wei Xu   +5 more
wiley   +1 more source

Controllable g5p-Protein-Directed Aggregation of ssDNA−Gold Nanoparticles

, 2016
We assembled single-stranded DNA (ssDNA) conjugated nanoparticles using the phage M13 gene 5 protein (g5p) as the molecular glue to bind two antiparallel noncomplementary ssDNA strands.
Oleg Gang (1412980), Yian-Biao Zhang (167916), Daniel van der Lelie (163216), Soo-Kwan Lee (2397169), Mathew M. Maye (1436833)   +4 more
core   +1 more source

Putative ssDNA contaminant

, 2021
Partial ssDNA virus genome assembled from fungal RCA library. Part of the sequence was trimmed off, since it closely matched an mRNA from the source fungus.
Cormac Kinsella (7853537)
core   +1 more source

SynNotch Receptors for Visualizing Immunoreceptor Force Transmission and Downstream Signaling In Vivo

Advanced Science, EarlyView.
A mechanically activated SynNotch reporter platform enables detection of force transmission through receptors as they mediate immune cell interactions. Targeting CD40 on B cells and TCR on T cells records force‐induced reporter activation across 2D coculture, 3D organoid, and implanted in mice, which also reveal force‐amplification of immune receptor ...
Menglan Li, Jintian Lyu, Kaitao Li, Ameya Dravid, Deepali Balasubramani, Amir H. K. Ashkezari, Hyun‐Kyu Choi, Gabriel A. Kwong, Ankur Singh, Cheng Zhu   +9 more
wiley   +1 more source

Flavobakteereita infektoivan ssDNA-bakteriofagi V155:n karakterisointi

, 2014
ssDNA-bakteriofageja esiintyy ympäristössä huomattavasti enemmän, kuin mitä on aiemmin oletettu. Viime aikoina kehittyneet virusten laskentametodit ovatkin aiheuttaneet ainoan tunnetun ssDNA-faagiperheen, Microviridae:n, nopean kasvun ja ...
Marjakangas, Jenni
core   +1 more source

Gel shift assay of TrmBL2 binding to ssDNA and dsDNA.

, 2016
The lanes contained TrmBL2 at increasing concentration (solid black ramp) and DNA. (A) Gel of ssDNA incubated with TrmBL2 at different concentrations. Marked with arrows are the ssDNA/TrmBL2 complex (top) and the ssDNA alone (bottom).
Winfried Boos (2805121), Ralf Seidel (17356), Malte Drescher (1456363), Misbha Ud Din Ahmad (2805118), Sebastian Wierer (2805112), Wolfram Welte (1371657), Peter Daldrop (2805115)   +6 more
core   +1 more source

Thermodynamics of hRPA-ssDNA binding.

, 2023
(A) 1D free energy landscape of hRPA-ssDNA binding as a function of the distance root-mean-square deviation of hRPA-ssDNA binding distances drms, between the hRPA and ssDNA beads that are in contact for flexible (red colored) and rigid hRPA model (blue ...
Arnab Bhattacherjee (476141), Sangeeta (14475348)   +1 more
core   +1 more source