Results 101 to 110 of about 53,634 (224)

Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects

Interdisciplinary Medicine, EarlyView.
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou, Xuemei Ren, Yingjie Ren, Ruiping Wu, Ruiman Geng, Le Chen, Zhuo Li   +6 more
wiley   +1 more source

Noncanonical Nucleotides in the Genome Around the Maternal‐Zygotic Transition

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
In this paper, Kazzazi et al. provide a comprehensive review of the dynamics of nonconventional nucleotides in the genome during early developmental stages, hypothesizing a potential role for these nucleotides in the activation of the zygotic genome. ABSTRACT From the very moment of fertilization and throughout development, the cells of animal embryos ...
Latifa Kazzazy, Dávid Mező, Kinga K. Nagy, Viktória Perey‐Simon, Judit Tóth, Angéla Békési, Beáta Vértessy, Máté Varga   +7 more
wiley   +1 more source

Bacterial exonuclease III expands its enzymatic activities on single-stranded DNA

eLife
Bacterial exonuclease III (ExoIII), widely acknowledged for specifically targeting double-stranded DNA (dsDNA), has been documented as a DNA repair-associated nuclease with apurinic/apyrimidinic (AP)-endonuclease and 3′→5′ exonuclease activities.
Hao Wang, Chen Ye, Qi Lu, Zhijie Jiang, Chao Jiang, Chun Zhou, Na Li, Caiqiao Zhang, Guoping Zhao, Min Yue, Yan Li   +10 more
doaj   +1 more source

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

Ultrasensitive MicroRNA Detection Combining Reduced Graphene Oxide Electrolyte‐Gated Transistors and Machine Learning

Small, EarlyView.
We developed an ultrasensitive and highly selective biosensor by integrating bio‐functionalized reduced graphene oxide (rGO) electrolyte‐gated transistors (EGTs) and machine learning (ML) to detect miRNAs (miR‐34a, miR‐34b, and miR‐34c) associated with cancer and neurological disorders.
Ana Vitória Ferreira Deleigo, Gabrielle Coelho Lelis, Maria Luisa Braunger, Stefano Casalini, Yasmin Watanabe, Gabriel Ravanhani Schleder, Wilson Tiago Fonseca, Rafael Furlan de Oliveira   +7 more
wiley   +1 more source

DNA‐Enzyme Hybrid Nanostructures: Functional Materials to Modulate Enzymatic Activity

Small, EarlyView.
DNA–enzyme hybrid nanostructures enable precise spatial and stoichiometric control over enzyme organization, offering a powerful platform to modulate catalytic activity. This review critically evaluates key mechanistic hypotheses, including proximity effects, microenvironment changes, confinement, and stabilization, as well as highlighting ...
Manar Elnaggar, Amelie Heuer‐Jungemann
wiley   +1 more source

Epx4 Nanopore With Multiple Constrictions for Single‐Molecule Identification

Small Methods, EarlyView.
Epx4 is a biological multi‐constriction nanopore that generates informative current signals for polypeptide sensing. Compared with α‐hemolysin, Epx4 detects short poly‐L‐lysine (S‐PLL, Mw 10 kDa) at a higher event frequency and produces signal features that distinguish it from long poly‐L‐lysine (L‐PLL, Mw 30–70 kDa), with an ROC AUC of 0.82 for the ...
Ayako Ijuin, Kota Naito, Mana Sato, Virginia Di Toro Mammarella, Nanami Takeuchi, Mauro Chinappi, Yoshikazu Tanaka, Ryuji Kawano   +7 more
wiley   +1 more source

Two‐Dimensional Materials For Lateral Flow Assays: A Critical Review of Functional Roles and Translational Challenges

VIEW, EarlyView.
2D nanosheets, with large surface area, excellent optical/electrical properties, and high specificity, combine with lateral flow assay strips to enable rapid, readable detection, boosting the strips’ development and applications. Abstract Lateral flow assays (LFAs) are widely used for point‐of‐care diagnostics, but their detection capability remains ...
Weimin Xu, Ruping Liu, Na Gao, Jianxiao Zhu, Haowen Jiang, Shouzheng Jiao, Zhicheng Sun, Kai Shen, Gaolian Xu, Minghua Du, Ziyan Li, Yang Wang   +11 more
wiley   +1 more source

ssDNA Tools [PDF]

Genome Biology, 2001
openaire   +1 more source

Effect of the microbiome on pathogen susceptibility across four Drosophilidae species

Insect Science, EarlyView.
Four Drosophilidae species were used to investigate how variation in the host microbiome influences susceptibility to infection. Microbial composition and abundance differed among species and treatments. The effects of microbiome manipulation on host survival were both species‐ and pathogen‐specific.
Hongbo Sun, Ben Longdon, Ben Raymond
wiley   +1 more source