Results 101 to 110 of about 53,752 (247)

Noncanonical Nucleotides in the Genome Around the Maternal‐Zygotic Transition

open access: yesJournal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
In this paper, Kazzazi et al. provide a comprehensive review of the dynamics of nonconventional nucleotides in the genome during early developmental stages, hypothesizing a potential role for these nucleotides in the activation of the zygotic genome. ABSTRACT From the very moment of fertilization and throughout development, the cells of animal embryos ...
Latifa Kazzazy   +7 more
wiley   +1 more source

Harnessing Aggregation‐Induced Emission for Advanced Disease Diagnosis: Mechanisms, Strategies and Future Perspectives

open access: yesMedicine Bulletin, EarlyView.
The figure was created in BioRender.com. ABSTRACT Accurate and early diagnosis remains a key challenge in modern disease management. Conventional diagnostic methods often suffer from limitations in sensitivity, spatial resolution, and practical usability.
Zizhuo Du   +10 more
wiley   +1 more source

Bacterial exonuclease III expands its enzymatic activities on single-stranded DNA

open access: yeseLife
Bacterial exonuclease III (ExoIII), widely acknowledged for specifically targeting double-stranded DNA (dsDNA), has been documented as a DNA repair-associated nuclease with apurinic/apyrimidinic (AP)-endonuclease and 3′→5′ exonuclease activities.
Hao Wang   +10 more
doaj   +1 more source

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

open access: yesPest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian   +6 more
wiley   +1 more source

Ultrasensitive MicroRNA Detection Combining Reduced Graphene Oxide Electrolyte‐Gated Transistors and Machine Learning

open access: yesSmall, EarlyView.
We developed an ultrasensitive and highly selective biosensor by integrating bio‐functionalized reduced graphene oxide (rGO) electrolyte‐gated transistors (EGTs) and machine learning (ML) to detect miRNAs (miR‐34a, miR‐34b, and miR‐34c) associated with cancer and neurological disorders.
Ana Vitória Ferreira Deleigo   +7 more
wiley   +1 more source

Thermally Regulated Droplet Microfluidics Enables Uniform, Size‐Controllable DNA‐Nanoparticle Superlattices

open access: yesSmall, EarlyView.
Thermally regulated droplet microfluidics enables controlled assembly of DNA‐functionalized gold nanoparticles into uniform, size‐controllable superlattice crystals. Monodisperse droplets formed at elevated temperature suppress premature hybridization, followed by ultra‐slow cooling to promote near‐equilibrium growth.
Naotomo Tottori   +6 more
wiley   +1 more source

Precisely Engineering Biomimetic Nanoparticles via Controlled Cell Membrane Coating

open access: yesSmall, EarlyView.
Precise engineering of cell membrane‐coated nanoparticles (CMNPs) requires coordinated control over particle size, coating integrity, and membrane leaflet orientation. This review integrates key synthesis parameters, assembly strategies, and analytical methods that govern these features, revealing how structural control underpins biomimetic function ...
Jie Lay Lim   +4 more
wiley   +1 more source

How We Simulate Nanopores

open access: yesSmall Methods, EarlyView.
Nanopore technology has revolutionized single‐molecule sensing, yet it suffers from an inherent interpretive challenge. Molecular dynamics has emerged as a go‐to method for determining the relationship between the structure of translocating molecules and the nanopore current signals.
Behzad Mehrafrooz   +5 more
wiley   +1 more source

Two‐Dimensional Materials For Lateral Flow Assays: A Critical Review of Functional Roles and Translational Challenges

open access: yesVIEW, EarlyView.
2D nanosheets, with large surface area, excellent optical/electrical properties, and high specificity, combine with lateral flow assay strips to enable rapid, readable detection, boosting the strips’ development and applications. Abstract Lateral flow assays (LFAs) are widely used for point‐of‐care diagnostics, but their detection capability remains ...
Weimin Xu   +11 more
wiley   +1 more source

ssDNA Tools [PDF]

open access: yesGenome Biology, 2001
openaire   +1 more source

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