Results 111 to 120 of about 66,303 (280)

Multi‐Technique Integration Identifies O‐GlcNAc Transferase in Fibroblast‐Like Synoviocytes as a Therapeutic Target for Rheumatoid Arthritis

Advanced Science, EarlyView.
AI‑guided single‑cell transcriptomics identifies OGT as a key regulator enriched in rheumatoid arthritis fibroblast‑like synoviocytes (RA‐FLSs). Inflammatory stimuli activate the OGT–SAP130 axis, which modulates the H3K27ac/H3K27me3 balance and epigenetically silences BTG2, promoting aggressive transformation of RA‑FLSs.
Duoli Xie, Jie Huang, Chunhao Cao, Zhenchao Tang, Xingchen Li, Jianmin Guo, Xinxin Chen, Fang Wang, Dongyi He, Jie Li, Yu Du, Bing He, Jianhua Yao, Aiping Lu, Chao Liang   +14 more
wiley   +1 more source

Matrix‐Activated Aptamer Sensors for Human Serum Analysis

Angewandte Chemie, EarlyView.
The sample self‐analyzes: The predominant serum proteins, albumin and immunoglobulin G, were leveraged to activate the response of fluorescent aptamer probes within the biological matrix. The fluorescence‐anisotropy‐based sensing platform demonstrated both performance and consistency for the direct detection of small molecules in serum‐rich samples ...
Amandine Barbaroux, Benoit Chovelon, Emmanuelle Fiore, Sebastien Vezirian, Mickael Henry, Marwa Zoubeidi, Maria C. DeRosa, Corinne Ravelet, Eric Peyrin   +8 more
wiley   +2 more sources

Interaction Between Gold Nanoparticles and ssDNA

, 2009
The interaction between unmodified gold nanoparticles and single-stranded DNA (ssDNA) was studied at different pH value. And the sequence-dependent stability of ssDNA-GNPs complex was investigated.
Wang, X. Y., 张其清, Zhang, J. F., Sun, L. P., Wang, S., Zhang, Q. Q., Li, H., Zhang, Z. W.   +7 more
core   +2 more sources

Strategies and considerations for the generation of ssDNA-Based HDR templates for CRISPR-based genome editing

BMC Genomics
The usefulness of genome editing lies in the ability to induce any desirable change in the target genome. It is most efficiently achieved using ssDNA (single-stranded DNA) as the HDR template and CRISPR-Cas9 targeted DNA breaks.
Harshitha Golagana Velangani, Aditi Ghosh, Sudiksha Singh, Shashi Kiran   +3 more
doaj   +1 more source

Selection and targeting of EpCAM protein by ssDNA aptamer

PLOS ONE, 2017
Aptamers are molecules that reveal highly complex and refined molecular recognition properties. These molecules are capable of binding with high affinity and selectivity to targets, ranging from small molecules to whole living cells. Several aptamers have been selected for targeting cellular proteins and they have also used in developing therapeutics ...
Walhan Alshaer, Nida Ababneh, Mamon Hatmal, Heba Izmirli, Moujab Choukeife, Alaa Shraim, Nour Sharar, Aya Abu-Shiekah, Fadwa Odeh, Abeer Al Bawab, Abdalla Awidi, Said Ismail   +11 more
openaire   +4 more sources

TECTB Variants Reveal Tectorial Membrane Vulnerability in Dominant Non‐Syndromic Hearing Loss

Advanced Science, EarlyView.
TECTB is a non‐collagenous protein of the tectorial membrane – an extracellular matrix of the cochlea. This study identifies dominant missense variants in TECTB linked to human hereditary deafness in two unrelated families. Genetically engineered mice homozygous for one of the variants are profoundly deaf, whereas heterozygous mice have normal hearing ...
Evan B. Hale, Barbara Vona, Richard J. Goodyear, Richard T. Osgood, Jun Shen, Sami S. Amr, Karen Mojica, Michaela A. H. Hofrichter, Jörg Schröder, Sophie Flandin, Paul Gratias, Ricardo Vera‐Monroy, Katherine Callahan, Kerry L. Gudlewski, Rolen Quadros, Masato Ohtsuka, JoAnn McGee, Edward J. Walsh, Cynthia C. Morton, Thomas Haaf, Channabasavaiah Gurumurthy, James E. Saunders, Guy P. Richardson, Artur A. Indzhykulian   +23 more
wiley   +1 more source

Analyses for TcaR-viral ssDNA complex.

, 2013
(A) EMSA analysis of the binding of TcaR and other DNA binding proteins to Viral M13 and φX174 ssDNA. Viral M13 and φX174 ssDNA (12 µM nucleotides) was incubated with an increasing concentration of TcaR, IcaR or SAR2349 at 30°C for 15 min.
Cammy K. -M. Chen (306222), Yuan-Chih Chang (29676), Wen-Yih Jeng (306223), Yu-Ming Chang (306221), Ming-Hon Hou (7694), Andrew H. -J. Wang (115232)   +5 more
core   +1 more source

Nrsn1–Smarcc1 Coupling Regulates Neural Stem Cell Differentiation and Chronic‐phase Recovery After Ischemic Stroke

Advanced Science, EarlyView.
In the post‐stroke brain, Foxa2 induces the transcriptional upregulation of Nrsn1 in NSCs. Nrsn1 functionally couples with Smarcc1, modulating its nuclear availability and protein abundance, thereby influencing Smarcc1‐associated regulatory programs linked to neuronal lineage commitment. Through this coupling, Nrsn1 promotes the differentiation of NSCs
Ruolin Zhang, Chang Liu, Yuneng Zhou, Kaichen Zhao, Muyang Li, Bingcheng Cai, Ying Wang, Zhiyuan Yuan, Zhaoxin Liu, Zilong Yuan, Yao Xiao, Peiyang Zhou, Ke Shui, Wendai Bao, Min Zhang, Jun Qin, Jun Chen, Xin Yang, Zhiqiang Dong   +18 more
wiley   +1 more source

Dynamic Operations in Macromolecular Data Storage

Angewandte Chemie, EarlyView.
With the exponential growth of digital data and the limitations of conventional silicon‐based storage and computing technologies, macromolecular data storage, the practice of encoding digital information within large, complex molecules like DNA or synthetic polymers, has emerged as a scalable and sustainable alternative to traditional digital ...
Jakub Ossowski, Oskar Affenberger, Jean‐François Lutz, Maria Nerantzaki   +3 more
wiley   +2 more sources

Detection results of Sequence-specificity ssDNA in different surroundings.

, 2014
(A). Fluorescence intensities of three viruses ssDNA and their hybridized products after S1 nuclease-mediated cleavage using SYBR Gold (1×). The concentration of target ssDNAs are 50 nM, and their complementary ssDNAs are 100 nM.
Jinchuan Liu (438592), Wenhui Bai (639384), Zheng Guan (438591), Xiaoling Jiang (158170), Shuming Yang (107864), Ailiang Chen (107859), Guiyuan Lv (640426), Zhenzhen Lv (512071)   +7 more
core   +1 more source