Results 51 to 60 of about 66,303 (280)
APOBEC3G Interacts with ssDNA by Two Modes: AFM Studies. [PDF]
, 2015 APOBEC3G (A3G) protein has antiviral activity against HIV and other pathogenic retroviruses. A3G has two domains: a catalytic C-terminal domain (CTD) that deaminates cytidine, and a N-terminal domain (NTD) that binds to ssDNA.
Li, Ming +5 more
core +1 more source
Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma [PDF]
Sensors, 2013 As a “chemical antibody”, oligonucleotide aptamers can specifically bind to their target molecules. However, clinical potential of aptamers in disease diagnosis is not yet fully explored. Using a tumor cell-based selection protocol, we developed single-stranded DNA aptamers for Hodgkin lymphoma (HL) tumor cells.
Parekh, Parag +6 more
openaire +3 more sources
, 2013 Non-denaturing gel electrophoresis (3.5–5–12%) was used to confirm SSB protein binding to ssDNA. Relative dsDNA was used as a binding control. M, monomer; D, dimer; T, tetramer. A, ssob binding ssDNA analysis. B, tth binding ssDNA analysis.
Haiyan Shi (287127) +10 more
core +1 more source
Finding novel vulnerabilities of hypomorphic BRCA1 alleles
Molecular Oncology, EarlyView.Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder +10 more
wiley +1 more source
ssDNA sequencing by rectification
, 2018 26 pages, 15 ...
Djurišić, Ivana +5 more
openaire +2 more sources
Conserved catalytic residues for dsDNA and ssDNA cleavage.
, 2023 (A) Conserved catalytic residues for DNA cleavage in apoCas12a-crRNA (left) and Lb2Cas12a-crRNA (right). (B) Catalytic residues for dsDNA cleavage, C: Control, dsDNA only, S: Substrate dsDNA, P: Cleaved products.
Sun Meng (11007860) +8 more
core +1 more source
Molecular Oncology, EarlyView.The kinase SRPK1 directly interacts with the protein TOPBP1 and regulates the pre‐mRNA splicing of WIZ thereby contributing to the activation of the ATR/CHK1 replicative checkpoint in response to replicative stress. This allows cancer cells' genomic stability and survival.
Amani Shreim +17 more
wiley +1 more source
Bioengineering, 2022 Poly(ethylene glycol) (PEG) films, fabricated by thermally induced crosslinking of amine- and epoxy-terminated four-arm STAR-PEG precursors, were used as porous and bioinert matrix for single-stranded DNA (ssDNA) immobilization and hybridization.
Zhiyong Zhao +2 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
ssDNA (oligo) recombineering model.
, 2018 A) Synaptases (pink balls) bind to ssDNA (represented by the green left-facing arrow at top) to form a nucleoprotein filament. B) The nucleoprotein filament samples ssDNA sequences between Okazaki fragments in replicating DNA.
Melvys Valledor (5634773) +2 more
core +1 more source