Results 51 to 60 of about 53,752 (247)
Negative supercoiling creates single-stranded patches of DNA that are substrates for AID-mediated mutagenesis. [PDF]
PLoS Genetics, 2012 Antibody diversification necessitates targeted mutation of regions within the immunoglobulin locus by activation-induced cytidine deaminase (AID). While AID is known to act on single-stranded DNA (ssDNA), the source, structure, and distribution of these ...
Jahan-Yar Parsa +8 more
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Biosensors, 2023 Exonuclease III (Exo III) has been generally used as a double-stranded DNA (dsDNA)-specific exonuclease that does not degrade single-stranded DNA (ssDNA). Here, we demonstrate that Exo III at concentrations above 0.1 unit/μL can efficiently digest linear
Yi Shen +5 more
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Programmable Functional Silicification of DNA Origami Nanostructures
Advanced Materials, EarlyView.This work introduces additional functionality into silica‐coated DNA origami nanostructures using non‐standard silica precursors. A fluorescent precursor enables enhanced intracellular tracking, while a disulfide‐containing reagent yields redox‐responsive, degradable silica coatings.
Anna V. Baptist +4 more
wiley +1 more source
eLife, 2023 Cells have evolved the DNA damage response (DDR) pathways in response to DNA replication stress or DNA damage. In the ATR-Chk1 DDR pathway, it has been proposed that ATR is recruited to RPA-coated single-stranded DNA (ssDNA) by direct ATRIP-RPA ...
Yunfeng Lin +5 more
doaj +1 more source
Advanced Science, EarlyView.Anti‐PD‐1/PD‐L1 blockade has revolutionized cancer immunotherapy, but is ineffective against endocrine‐treated (i.e., Tamoxifen), relapsed ER+ breast cancer (BC) patients. This study provides insight into the sub‐optimal response of ER+BCs to anti‐PD‐1/PD‐L1 blockade – highlighting the induction of STING and the CEACAM1/TIM3 axis after chronic ...
Marvin Angelo E Aberin +20 more
wiley +1 more source
Managing Single-Stranded DNA during Replication Stress in Fission Yeast
Biomolecules, 2015 Replication fork stalling generates a variety of responses, most of which cause an increase in single-stranded DNA. ssDNA is a primary signal of replication distress that activates cellular checkpoints. It is also a potential source of genome instability
Sarah A. Sabatinos, Susan L. Forsburg
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Archaeal Orc1 protein interacts with T-rich single-stranded DNA
BMC Research Notes, 2021 Objective The ability to form nucleoprotein complexes is a fundamental activity of DNA replication initiation proteins. They bind within or nearby the region of replication origin what results in melting of a double-stranded DNA (dsDNA) and formation of ...
Katarzyna Wegrzyn, Igor Konieczny
doaj +1 more source
QRB Discovery, 2022 Interactions between proteins and single-stranded DNA (ssDNA) are crucial for many fundamental biological processes, including DNA replication and genetic recombination.
Krzysztof Kubiak +10 more
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, 2019 ssDNA Extraction Protocol This protocol is based on the Oligonucleotide extraction protocol of the NEB PCR & Clean-up Kit and the Qiagen DNeasy Protocol with a few modifications to maximize DNA output. First results of this protocol will be published soon, including first sequencing results of the so extracted DNA.
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Probing the mechanism of recognition of ssDNA by the Cdc13-DBD [PDF]
Nucleic Acids Research, 2008 The Saccharomyces cerevisiae protein Cdc13 tightly and specifically binds the conserved G-rich single-stranded overhang at telomeres and plays an essential role in telomere end-protection and length regulation. The 200 residue DNA-binding domain of Cdc13 (Cdc13-DBD) binds an 11mer single-stranded representative of the yeast telomeric sequence [Tel11, d(
Eldridge, Aimee M., Wuttke, Deborah S.
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