Results 41 to 50 of about 53,752 (247)

Mechanism of Dynamic Binding of Replication Protein A to ssDNA [PDF]

Journal of Chemical Information and Modeling, 2020
Abstract Replication protein A (RPA) serves as hub protein inside eukaryotic cells, where it coordinates crucial DNA metabolic processes and activates the DNA-damage response system. A characteristic feature of its action is to associate with ssDNA intermediates before handing over them to downstream proteins.
Anupam Mondal, Arnab Bhattacherjee
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Rational Design of Porous Poly(ethylene glycol) Films as a Matrix for ssDNA Immobilization and Hybridization

Bioengineering, 2022
Poly(ethylene glycol) (PEG) films, fabricated by thermally induced crosslinking of amine- and epoxy-terminated four-arm STAR-PEG precursors, were used as porous and bioinert matrix for single-stranded DNA (ssDNA) immobilization and hybridization.
Zhiyong Zhao, Saunak Das, Michael Zharnikov   +2 more
doaj   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

Interdomain dynamics in human Replication Protein A regulates kinetics and thermodynamics of its binding to ssDNA

PLoS ONE, 2023
Human Replication Protein A (hRPA) is a multidomain protein that interacts with ssDNA intermediates to provide the latter much-needed stability during DNA metabolism and maintain genomic integrity.
Sangeeta, Arnab Bhattacherjee
doaj  

Coordinated Binding of Single-Stranded and Double-Stranded DNA by UvsX Recombinase. [PDF]

PLoS ONE, 2013
Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/Rad51 family perform the central catalytic role in this process.
Robyn L Maher, Scott W Morrical
doaj   +1 more source

TheBorrelia burgdorferitelomere resolvase, ResT, anneals ssDNA complexed with its cognate ssDNA-binding protein [PDF]

Nucleic Acids Research, 2016
Spirochetes of the genus Borrelia possess unusual genomes that consist in a linear chromosome and multiple linear and circular plasmids. The linear replicons are terminated by covalently closed hairpin ends, referred to as hairpin telomeres. The hairpin telomeres represent a simple solution to the end-replication problem.
Huang, Shu Hui, Kobryn, Kerri
openaire   +2 more sources

DNA‐Templated 2D Heterostructures as Phototriggered Dynamic Nanohybrids: From Releasing Molecular Loads to Controlling Enzyme Biocatalytic Function

Advanced Functional Materials, EarlyView.
DNA strands are employed both as dynamic linkers and nanoscale templates for the integration of Ag2S nanoparticles on MoS2, which in turn imparted photothermal responsiveness; this feature permits the selective cargo (fluorophore, quantum dots or an enzyme) release from the MoS2 surface in response to local heat induced by light irradiation.
Kai Chen, Haosen Miao, Weiying Hong, Matteo Palma   +3 more
wiley   +1 more source

Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives

Advanced Materials, EarlyView.
Kelvin probe force microscopy (KPFM) enables the nanoscale mapping of electrostatic surface potentials. While widely applied in materials science, its use in biological systems remains emerging. This review presents recent advances in KPFM applied to biological samples and provides a critical perspective on current limitations and future directions for
Ehsan Rahimi, Mario Palacios‐Corella, Arjan Mol, Salvador Pané, Josep Puigmartí‐Luis   +4 more
wiley   +1 more source

Magnetic DNA Origami Nanorotors

Advanced Materials, EarlyView.
Magnetic actuation is a powerful and broadly applicable actuation mechanism due to its programmability and compatibility with biological entities. Here we demonstrate magnetic DNA origami nanorotors (MADONAs) by assembling magnetic nanocubes on DNA origami.
Lennart J. K. Weiß, Florian Rothfischer, Yihao Wang, Christoph Pauer, Xin Yin, Kevin Lang, Rabia Amin, Thomas Tsalos, Susanne Kempter, Jan Lipfert, Tim Liedl, Friedrich C. Simmel, Joe Tavacoli, Aidin Lak   +13 more
wiley   +1 more source