Results 41 to 50 of about 66,303 (280)

Label-free and sensitive fluorescent detection of sequence-specific single-strand DNA based on S1 nuclease cleavage effects. [PDF]

PLoS ONE, 2014
The ability to detect sequence-specific single-strand DNA (ssDNA) in complex, contaminant-ridden samples, using a fluorescent method directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic ...
Zheng Guan, Jinchuan Liu, Wenhui Bai, Zhenzhen Lv, Xiaoling Jiang, Shuming Yang, Ailiang Chen, Guiyuan Lv   +7 more
doaj   +1 more source

Profiling a single-stranded DNA region within an rDNA segment that affects the loading of bacterial condensin

iScience, 2022
Summary: Bacterial condensin preferentially loads onto single-stranded DNA (ssDNA) in vitro and onto rDNA in vivo to support proper chromosome compaction.
Koichi Yano, Hideki Noguchi, Hironori Niki   +2 more
doaj   +1 more source

Acousto-microfluidics for screening of ssDNA aptamer [PDF]

Scientific Reports, 2016
AbstractWe demonstrate a new screening method for obtaining a prostate-specific antigen (PSA) binding aptamer based on an acoustofluidic separation (acoustophoreis) technique. Since acoustophoresis provides simultaneous washing and separation in a continuous flow mode, we efficiently obtained a PSA binding aptamer that shows high affinity without any ...
Park, Jee-Woong, Lee, Su Jin, Ren, Shuo, Lee, Sangwook, Kim, Soyoun, Laurell, Thomas   +5 more
openaire   +3 more sources

Electrochemical and Spectroscopic Study of Samarium Ion Interaction with DNA in Different pHs

International Journal of Electrochemical Science, 2010
In this work, for the first time the electrochemical behavior of the Sm3+ and Sm3+ ion interaction with short single strand DNA (ssDNA) sequence in two pHs was studied. Then the UV-Vis spectroscopic method was used for supporting these evidences.
H. Ilkhani, M.R. Ganjali, M. Arvand, P. Norouzi   +3 more
doaj   +1 more source

ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells

BMC Genomics, 2023
Background Recent advances in CRISPR technology have enabled us to perform gene knock-in in various species and cell lines. CRISPR-mediated knock-in requires donor DNA which serves as a template for homology-directed repair (HDR).
Akira Mabuchi, Shoji Hata, Mariya Genova, Chiharu Tei, Kei K. Ito, Masayasu Hirota, Takuma Komori, Masamitsu Fukuyama, Takumi Chinen, Atsushi Toyoda, Daiju Kitagawa   +10 more
doaj   +1 more source

Conformational Changes in Ff Phage Protein gVp upon Complexation with Its Viral Single-Stranded DNA Revealed Using Magic-Angle Spinning Solid-State NMR

Viruses, 2022
Gene V protein (gVp) of the bacteriophages of the Ff family is a non-specific single-stranded DNA (ssDNA) binding protein. gVp binds to viral DNA during phage replication inside host Escherichia coli cells, thereby blocking further replication and ...
Smadar Kedem, Roni Rene Hassid, Yoav Shamir, Amir Goldbourt   +3 more
doaj   +1 more source

ssDNA-binding of HsRecA L53Q, ATPase and DNA strand exchange activity promoted by EcRecA, wild-type HsRecA and HsRecA L53Q proteins.

, 2019
(A) ssDNA-binding capacity of HsRecA L53Q assayed by using [α32P]-ssDNA 88 mers. (B) Amounts of ATP hydrolyzed. The ATPase activity profile of wild-type HsRecA was previously characterized [15,24]. (C) The three DNA strand exchange activity.
Jorge Iulek (368971), Renato F. Penteado (6612035), Fernando Gomes (6143702), Rafael M. Etto (2889869), Wellington C. Leite (2889878), Carolina W. Galvão (2889872), Sérgio C. Saab (2889881), Maria B. R. Steffens (462794)   +7 more
core   +1 more source

RADA preferentially binds ssDNA.

, 2022
(A) EMSA experiments showing that RADA binds to any ssDNA-containing DNA structure with higher affinity than to dsDNA. Lower and higher molecular weight complexes are indicated by white and black arrowheads respectively.
Frédérique Weber-Lotfi (12537055), Anaïs Le Blevenec (12537058), Marc Bichara (324546), José M. Gualberto (11183568), Arnaud Fertet (11183562), Cédric Nadiras (12537061), Nicolas Chevigny (12537052), Cécile Raynaud (258874), André Dietrich (11402013), Mathieu Erhardt (8270)   +9 more
core   +1 more source

Anti‐ssDNA and dsDNA Antibodies in Preeclampsia

Asia-Oceania Journal of Obstetrics and Gynaecology, 1994
AbstractThe presence of anti‐ssDNA and anti‐dsDNA antibodies and their clinical significance in preeclampsia were studied. Serum samples were taken from 38 cases of preeclampsia including 19 severe cases, and from 26 normal pregnant women. Anti‐ssDNA and anti‐dsDNA antibodies were measured by using an enzyme‐linked im‐munosorbent assay (ELISA).Only 1 ...
T, Yamamoto, S, Yoshimura, Y, Geshi, Y, Sasamori, H, Mori, T, Kobayashi   +5 more
openaire   +3 more sources

SV40 T antigen helicase domain regions responsible for oligomerisation regulate Okazaki fragment synthesis initiation

FEBS Open Bio, 2022
The initiation of Okazaki fragment synthesis during cellular DNA replication is a crucial step for lagging strand synthesis, which is carried out by the primase function of DNA polymerase α‐primase (Pol‐prim).
Nichodemus O. Onwubiko, Felicia Scheffel, Ingrid Tessmer, Heinz Peter Nasheuer   +3 more
doaj   +1 more source