Results 21 to 30 of about 53,752 (247)

Gas Biosensor Arrays Based on Single-Stranded DNA-Functionalized Single-Walled Carbon Nanotubes for the Detection of Volatile Organic Compound Biomarkers Released by Huanglongbing Disease-Infected Citrus Trees

open access: yesSensors, 2019
Volatile organic compounds (VOCs) released by plants are closely associated with plant metabolism and can serve as biomarkers for disease diagnosis. Huanglongbing (HLB), also known as citrus greening or yellow shoot disease, is a lethal threat to the ...
Hui Wang   +6 more
doaj   +1 more source

A real-time fluorescent gp32 probe-based assay for monitoring single-stranded DNA-dependent DNA processing enzymes

open access: yesBiochemistry and Biophysics Reports, 2023
Single-stranded DNA (ssDNA) generated during DNA replication, recombination and damage repair reactions is an important intermediate and ssDNA-binding proteins that binds these intermediates coordinate various DNA metabolic processes. Mechanistic details
Senthil K. Perumal
doaj   +1 more source

The viral etiology of tomato yellow leaf curl disease - a review

open access: yesPlant Protection Science, 2009
Tomato yellow leaf curl disease (TYLCD) is one of the most devastating plant diseases in the world. As a result of its continuing rapid spread, it now afflicts more than 30 tomato growing countries in the Mediterranean basin, southern Asia, Africa, and ...
Efrat Glick, Yael Levy, Yedidya Gafni
doaj   +1 more source

Selection and identification of an ssDNA aptamer to NB4 cell [PDF]

open access: yesJournal of Clinical Laboratory Analysis, 2021
AbstractThis study was to find the aptamers with high affinity and specificity binding to acute promyelocytic leukemia (APL) NB4 cell line.These aptamers targeted NB4 cells were selected from a random single‐stranded DNA (ssDNA) library of systematic evolution of ligands by exponential enrichment (CELL‐SELEX). The binding rate of FITC‐ssDNA library and
Xian‐hui Zhang, Wei Wang, Xin Chen
openaire   +2 more sources

Single-molecule observation of ATP-independent SSB displacement by RecO in Deinococcus radiodurans

open access: yeseLife, 2020
Deinococcus radiodurans (DR) survives in the presence of hundreds of double-stranded DNA (dsDNA) breaks by efficiently repairing such breaks. RecO, a protein that is essential for the extreme radioresistance of DR, is one of the major recombination ...
Jihee Hwang   +6 more
doaj   +1 more source

Roles of C-Terminal Region of Yeast and Human Rad52 in Rad51-Nucleoprotein Filament Formation and ssDNA Annealing. [PDF]

open access: yesPLoS ONE, 2016
Yeast Rad52 (yRad52) has two important functions at homologous DNA recombination (HR); annealing complementary single-strand DNA (ssDNA) molecules and recruiting Rad51 recombinase onto ssDNA (recombination mediator activity).
Nilesh V Khade, Tomohiko Sugiyama
doaj   +1 more source

HIV restriction factor APOBEC3G binds in multiple steps and conformations to search and deaminate single-stranded DNA

open access: yeseLife, 2019
APOBEC3G (A3G), an enzyme expressed in primates with the potential to inhibit human immunodeficiency virus type 1 (HIV-1) infectivity, is a single-stranded DNA (ssDNA) deoxycytidine deaminase with two domains, a catalytically active, weakly ssDNA binding ...
Michael Morse   +5 more
doaj   +1 more source

Acousto-microfluidics for screening of ssDNA aptamer [PDF]

open access: yesScientific Reports, 2016
AbstractWe demonstrate a new screening method for obtaining a prostate-specific antigen (PSA) binding aptamer based on an acoustofluidic separation (acoustophoreis) technique. Since acoustophoresis provides simultaneous washing and separation in a continuous flow mode, we efficiently obtained a PSA binding aptamer that shows high affinity without any ...
Park, Jee-Woong   +5 more
openaire   +3 more sources

Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathways

open access: yeseLife, 2015
Escherichia coli single-stranded (ss)DNA binding (SSB) protein mediates genome maintenance processes by regulating access to ssDNA. This homotetrameric protein wraps ssDNA in multiple distinct binding modes that may be used selectively in different DNA ...
Sukrit Suksombat   +4 more
doaj   +1 more source

Fluorescent single-stranded DNA-binding protein from Plasmodium falciparum as a biosensor for single-stranded DNA. [PDF]

open access: yesPLoS ONE, 2018
Single-stranded DNA (ssDNA) is a product of many cellular processes that involve double-stranded DNA, for example during DNA replication and repair, and is formed transiently in many others. Measurement of ssDNA formation is fundamental for understanding
Liisa T Chisty   +2 more
doaj   +1 more source

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