Results 11 to 20 of about 66,303 (280)

Details of ssDNA annealing revealed by an HSV-1 ICP8–ssDNA binary complex [PDF]

Nucleic Acids Research, 2013
Infected cell protein 8 (ICP8) from herpes simplex virus 1 was first identified as a single-strand (ss) DNA-binding protein. It is essential for, and abundant during, viral replication. Studies in vitro have shown that ICP8 stimulates model replication reactions, catalyzes annealing of complementary ssDNAs and, in combination with UL12 exonuclease ...
Tolun, Gökhan, Makhov, Alexander, Ludtke, Steven, Griffith, Jack D   +3 more
core   +7 more sources

Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA [PDF]

Cell Reports, 2014
The SOSS1 complex comprising SOSSA, SOSSB1, and SOSSC senses single-stranded DNA (ssDNA) and promotes repair of DNA double-strand breaks (DSBs). But how SOSS1 is assembled and recognizes ssDNA remains elusive. The crystal structure of the N-terminal half
Wendan Ren, Hongxia Chen, Qiangzu Sun, Xuhua Tang, Siew Choo Lim, Jun Huang, Haiwei Song   +6 more
doaj   +2 more sources

A Phage Single-Stranded DNA (ssDNA) Binding Protein Complements ssDNA Accumulation of a Geminivirus and Interferes with Viral Movement [PDF]

Journal of Virology, 1999
ABSTRACT Geminiviruses are plant viruses with circular single-stranded DNA (ssDNA) genomes encapsidated in double icosahedral particles. Tomato leaf curl geminivirus (ToLCV) requires coat protein (CP) for the accumulation of ssDNA in protoplasts and in plants but not for systemic infection and symptom development in plants.
Padidam, M., Beachy, R.N., /Fauquet, Claude   +2 more
openaire   +4 more sources

A comparative study of protein–ssDNA interactions [PDF]

NAR Genomics and Bioinformatics, 2021
Abstract Single-stranded DNA-binding proteins (SSBs) play crucial roles in DNA replication, recombination and repair, and serve as key players in the maintenance of genomic stability. While a number of SSBs bind single-stranded DNA (ssDNA) non-specifically, the others recognize and bind specific ssDNA sequences. The mechanisms underlying
Maoxuan Lin, Fareeha K Malik, Jun-tao Guo   +2 more
openaire   +2 more sources

Expression of ssDNA in Mammalian Cells [PDF]

BioTechniques, 2003
Antisense therapy involves the use of antisense oligonucleotides for altering targeted gene function. However, the low efficiency of cell delivery of antisense oligonucleotides has limited the efficacy of antisense therapeutic approaches. RNA-based antisense or ribozyme oligonucleotides can be either synthesized endogenously (e.g., by a viral vector ...
Yin Chen, Yong-Jie Ji, Charles Conrad
openaire   +3 more sources

DSS1 and ssDNA regulate oligomerization of BRCA2 [PDF]

Nucleic Acids Research, 2020
Abstract The tumor suppressor BRCA2 plays a key role in initiating homologous recombination by facilitating RAD51 filament formation on single-stranded DNA. The small acidic protein DSS1 is a crucial partner to BRCA2 in this process. In vitro and in cells (1,2), BRCA2 associates into oligomeric complexes besides also existing as monomers.
Hang Phuong Le, Xiaoyan Ma, Jorge Vaquero, Megan Brinkmeyer, Fei Guo, Wolf-Dietrich Heyer, Jie Liu   +6 more
openaire   +4 more sources

Molecular Detection and Determination of Phylogenetic Position of Watermelon Chlorotic Stunt Virus Khouzestan Isolate Based on Coat Protein Gene [PDF]

Journal of Genetic Resources
Watermelon chlorotic stunt virus (WmCSV) is a destructive Begomovirus from the family of Geminiviridae with a bipartite genome including similar-sized circular, single-stranded DNA (DNA-A and DNA-B) which has recently developed dangerously in many ...
Samira Pakbaz, Zahra Jelavi, Ehsan Hasanvand, Hossein Mirzaei Najafgholi   +3 more
doaj   +1 more source

Analysis of vibration behavior in single strand DNA-wrapped single-walled carbon nanotubes adhered to lipid membranes

Forces in Mechanics, 2021
Single strand DNA-wrapped Single-walled carbon nanotubes (ssDNA-SWCNTs) have critical biomedical applications such as drug delivery, bio-imaging and bio-sensing; therefore, the mechanical interaction between ssDNA-SWCNTs and lipid membranes is an ...
Daisuke Miyashiro, Ryo Hamano, Hisao Taira, Kazuo Umemura   +3 more
doaj   +1 more source

Structure of the hDmc1-ssDNA filament reveals the principles of its architecture [PDF]

, 2010
In eukaryotes, meiotic recombination is a major source of genetic diversity, but its defects in humans lead to abnormalities such as Down's, Klinefelter's and other syndromes.
Dmitry V Bugreev, Julie Hodgkinson, Yuriy L Chaban, Bugreev, DV, Orlova, Elena, Orlova, EV, Hodgkinson, J.L., Orlova Elena V., Chaban, Y.L., Bugreev, D.V., Andrei L Okorokov, Chaban Yuriy L., Okorokov, AL, Elena V Orlova, Okorokov, A.L., Hodgkinson, J, Bugreev Dmitry V., Chaban, YL, Okorokov Andrei L., Mazin, AV, Hodgkinson Julie, Mazin, A.V., Alexander V Mazin, Mazin Alexander V.   +23 more
core   +2 more sources

Protein-ssDNA model validation.

, 2023
The model has previously been used to capture the bound state conformations of a number of protein-ssDNA complexes (including SSB-ssDNA complex) irrespective of the length and sequences of ssDNA.
Arnab Bhattacherjee (476141), Sangeeta (14475348)   +1 more
core   +1 more source