Results 31 to 40 of about 53,752 (247)

Label-free and sensitive fluorescent detection of sequence-specific single-strand DNA based on S1 nuclease cleavage effects. [PDF]

PLoS ONE, 2014
The ability to detect sequence-specific single-strand DNA (ssDNA) in complex, contaminant-ridden samples, using a fluorescent method directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic ...
Zheng Guan, Jinchuan Liu, Wenhui Bai, Zhenzhen Lv, Xiaoling Jiang, Shuming Yang, Ailiang Chen, Guiyuan Lv   +7 more
doaj   +1 more source

Profiling a single-stranded DNA region within an rDNA segment that affects the loading of bacterial condensin

iScience, 2022
Summary: Bacterial condensin preferentially loads onto single-stranded DNA (ssDNA) in vitro and onto rDNA in vivo to support proper chromosome compaction.
Koichi Yano, Hideki Noguchi, Hironori Niki   +2 more
doaj   +1 more source

Electrochemical and Spectroscopic Study of Samarium Ion Interaction with DNA in Different pHs

International Journal of Electrochemical Science, 2010
In this work, for the first time the electrochemical behavior of the Sm3+ and Sm3+ ion interaction with short single strand DNA (ssDNA) sequence in two pHs was studied. Then the UV-Vis spectroscopic method was used for supporting these evidences.
H. Ilkhani, M.R. Ganjali, M. Arvand, P. Norouzi   +3 more
doaj   +1 more source

ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells

BMC Genomics, 2023
Background Recent advances in CRISPR technology have enabled us to perform gene knock-in in various species and cell lines. CRISPR-mediated knock-in requires donor DNA which serves as a template for homology-directed repair (HDR).
Akira Mabuchi, Shoji Hata, Mariya Genova, Chiharu Tei, Kei K. Ito, Masayasu Hirota, Takuma Komori, Masamitsu Fukuyama, Takumi Chinen, Atsushi Toyoda, Daiju Kitagawa   +10 more
doaj   +1 more source

Conformational Changes in Ff Phage Protein gVp upon Complexation with Its Viral Single-Stranded DNA Revealed Using Magic-Angle Spinning Solid-State NMR

Viruses, 2022
Gene V protein (gVp) of the bacteriophages of the Ff family is a non-specific single-stranded DNA (ssDNA) binding protein. gVp binds to viral DNA during phage replication inside host Escherichia coli cells, thereby blocking further replication and ...
Smadar Kedem, Roni Rene Hassid, Yoav Shamir, Amir Goldbourt   +3 more
doaj   +1 more source

Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA

Cell Reports, 2014
The SOSS1 complex comprising SOSSA, SOSSB1, and SOSSC senses single-stranded DNA (ssDNA) and promotes repair of DNA double-strand breaks (DSBs). But how SOSS1 is assembled and recognizes ssDNA remains elusive. The crystal structure of the N-terminal half
Wendan Ren, Hongxia Chen, Qiangzu Sun, Xuhua Tang, Siew Choo Lim, Jun Huang, Haiwei Song   +6 more
doaj   +1 more source

Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma [PDF]

Sensors, 2013
As a “chemical antibody”, oligonucleotide aptamers can specifically bind to their target molecules. However, clinical potential of aptamers in disease diagnosis is not yet fully explored. Using a tumor cell-based selection protocol, we developed single-stranded DNA aptamers for Hodgkin lymphoma (HL) tumor cells.
Parekh, Parag, Kamble, Sanchit, Zhao, Nianxi, Zeng, Zihua, Wen, Jianguo, Yuan, Bin, Zu, Youli   +6 more
openaire   +2 more sources

SV40 T antigen helicase domain regions responsible for oligomerisation regulate Okazaki fragment synthesis initiation

FEBS Open Bio, 2022
The initiation of Okazaki fragment synthesis during cellular DNA replication is a crucial step for lagging strand synthesis, which is carried out by the primase function of DNA polymerase α‐primase (Pol‐prim).
Nichodemus O. Onwubiko, Felicia Scheffel, Ingrid Tessmer, Heinz Peter Nasheuer   +3 more
doaj   +1 more source

ssDNA sequencing by rectification

, 2018
26 pages, 15 ...
Djurišić, Ivana, Dražić, Miloš S., Tomović, Aleksandar Ž., Spasenović, Marko, Jovanović, Vladimir P., Zikic, Radomir   +5 more
openaire   +2 more sources

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source