Results 221 to 230 of about 30,137 (254)
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Stable Isotopes Labeling of Drugs in Pediatric Clinical Pharmacology
Pediatrics, 1999Stable isotope labeling (SIL) still has not been used very much in pediatric and perinatal clinical pharmacology. However, this method has numerous advantages. It allows one to determine the concentration of drugs with great sensitivity, thereby allowing quantification in small amounts of biologic samples.
G, Pons, E, Rey
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Profiling of RNA modifications by multiplexed stable isotope labelling
Chemical Communications, 2014The combination of (15)N/(13)C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli and yeast, including 10 previously undescribed modifications. Two modifications, N-ribosylnicotinamide and 2-methylthioadenosine, were newly detected in species hitherto thought not to contain these modifications.
Stefanie Kellner +7 more
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Label-Free DNA Assay by Metal Stable Isotope Detection
Analytical Chemistry, 2017The interest in label-free bioassays is increasing rapidly because of their simple procedure and direct information on the interaction between the target molecule and the sensing unit. One of the major obstacles in the application of label-free biosensors is the difficulty to produce stable and reproducible optical, electric, electrochemical, or ...
Rui Liu +5 more
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Tandem mass spectrometry for measuring stable-isotope labeling
Current Opinion in Biotechnology, 2013Measuring metabolic rates by (13)C-metabolic flux analysis ((13)C-MFA) is of central importance for metabolic engineers and biomedical investigators. Enhanced knowledge of in vivo fluxes can be applied to reengineer the metabolic, regulatory, and phenotypic characteristics of organisms and help uncover the mechanisms of human ailments such as cancer ...
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Improving cell-free protein synthesis for stable-isotope labeling
Journal of Biomolecular NMR, 2007Cell-free protein synthesis is suitable for stable-isotope labeling of proteins for NMR analysis. The Escherichia coli cell-free system containing potassium acetate for efficient translation (KOAc system) is usually used for stable-isotope labeling, although it is less productive than other systems. A system containing a high concentration of potassium
Takayoshi, Matsuda +8 more
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Stable Isotope Labeling and Quantification of Photosynthetic Metabolites
Stable isotope labeling with 13CO2 coupled with mass spectrometry allows monitoring the incorporation of 13C into photosynthetic intermediates and is a powerful technique for the investigation of the metabolic dynamics of photosynthesis. We describe here a protocol for 13CO2 labeling of large leaved plants and of Arabidopsis thaliana rosette, and a ...Chiara Baccolini, Stéphanie Arrivault
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Kinetic equivalence of stable-isotope-labeled and unlabeled phenytoin
Clinical Pharmacology and Therapeutics, 1981Stable isotope labeling (SIL) of a drug results in a higher molecular weight than that of the unlabeled drug. SIL tracer doses can be quantitated separately from unlabeled drug by gas chromatography-mass spectrometry (GC-MS) without exposing the patient to radiation. The higher molecular weight of SIL drug could cause a higher energy of activation for (
T R, Browne +4 more
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Synthesis of stable isotope labeled PNU‐95666
Journal of Labelled Compounds and Radiopharmaceuticals, 2004AbstractStudies towards the synthesis of stable isotope labeled PNU‐95666, a CNS agent currently under development for the treatment of Parkinson's disease, is described. The synthesis of non‐labeled PNU‐95666 starts using a large excess of methylamine. While the non‐labeled methylamine is inexpensive and in plentiful supply, the stable isotope labeled
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IsoCorrectoR: Isotope correction in stable isotope labeling experiments in metabolomics
2022Im Metabolomics-Bereich können Experimente mit stabilen Isotopen-Labels wie ¹³C oder ¹⁵N sehr wertvolle Informationen liefern. Das schließt beispielsweise die Bestimmung von Beiträgen eines bestimmten Nährstoffs zur Synthese bestimmter Metabolite, Beiträge zu metabolischen Pathways, oder die Schätzung von metabolischen Fluxes mittels formaler Metabolic
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Quantitative Proteomics by Stable Isotope Labeling and Mass Spectrometry
2006The goal of quantitative proteomics is to systematically study static state or perturbation-induced changes in protein profile. Most of the recently developed mass spectrometry (MS)-based quantitative proteomic methods employ stable isotope labeling to introduce signature mass tags to peptides/proteins that can be used by a mass spectrometer to ...
Sheng, Pan, Ruedi, Aebersold
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