Results 221 to 230 of about 30,977 (246)
Some of the next articles are maybe not open access.
Stable Isotope Labeling and Quantification of Photosynthetic Metabolites
Stable isotope labeling with 13CO2 coupled with mass spectrometry allows monitoring the incorporation of 13C into photosynthetic intermediates and is a powerful technique for the investigation of the metabolic dynamics of photosynthesis. We describe here a protocol for 13CO2 labeling of large leaved plants and of Arabidopsis thaliana rosette, and a ...Chiara Baccolini, Stéphanie Arrivault
openaire +3 more sources
Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse
2014Since the first metabolic labeling experiments with stable isotopes beginning of the last century, several approaches were pursued to monitor protein dynamics in living animals. Today, almost all model organisms from bacteria to rodents can be fully labeled with SILAC (stable isotope labeling of amino acids in cell culture) amino acids. The development
Soraya, Hölper +2 more
openaire +2 more sources
Quantitative Proteomics by Stable Isotope Labeling and Mass Spectrometry
2006The goal of quantitative proteomics is to systematically study static state or perturbation-induced changes in protein profile. Most of the recently developed mass spectrometry (MS)-based quantitative proteomic methods employ stable isotope labeling to introduce signature mass tags to peptides/proteins that can be used by a mass spectrometer to ...
Sheng, Pan, Ruedi, Aebersold
openaire +2 more sources
Profiling of RNA modifications by multiplexed stable isotope labelling
Chemical Communications, 2014The combination of (15)N/(13)C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli and yeast, including 10 previously undescribed modifications. Two modifications, N-ribosylnicotinamide and 2-methylthioadenosine, were newly detected in species hitherto thought not to contain these modifications.
Stefanie Kellner +7 more
openaire +2 more sources
Analytical Chemistry, 2018
Stable-isotope labeling experiments (ILEs) are widely used to investigate the topology and operation of metabolic networks. The quality of isotopic data collected in ILEs is of utmost importance to ensure reliable biological interpretations, but current evaluation approaches are limited due to a lack of suitable reference material and relevant ...
Heuillet, Maud +5 more
openaire +4 more sources
Stable-isotope labeling experiments (ILEs) are widely used to investigate the topology and operation of metabolic networks. The quality of isotopic data collected in ILEs is of utmost importance to ensure reliable biological interpretations, but current evaluation approaches are limited due to a lack of suitable reference material and relevant ...
Heuillet, Maud +5 more
openaire +4 more sources
Tandem mass spectrometry for measuring stable-isotope labeling
Current Opinion in Biotechnology, 2013Measuring metabolic rates by (13)C-metabolic flux analysis ((13)C-MFA) is of central importance for metabolic engineers and biomedical investigators. Enhanced knowledge of in vivo fluxes can be applied to reengineer the metabolic, regulatory, and phenotypic characteristics of organisms and help uncover the mechanisms of human ailments such as cancer ...
openaire +2 more sources
Stable isotope labeling of nanomaterials for biosafety evaluation and drug development
Chinese Chemical Letters, 2022Xue-Ling Chang +2 more
exaly
Tracer or toxicant: Does stable isotope labeling affect central processes in aquatic food webs?
Aquatic Toxicology, 2023Eric Böllinger +2 more
exaly
Mass spectrometry of lipids labeled with stable isotopes
Progress in Lipid Research, 1985openaire +2 more sources