Results 31 to 40 of about 1,347 (156)

SIPSim: A Modeling Toolkit to Predict Accuracy and Aid Design of DNA-SIP Experiments

Frontiers in Microbiology, 2018
DNA Stable isotope probing (DNA-SIP) is a powerful method that links identity to function within microbial communities. The combination of DNA-SIP with multiplexed high throughput DNA sequencing enables simultaneous mapping of in situ assimilation ...
Nicholas D. Youngblut, Samuel E. Barnett, Daniel H. Buckley   +2 more
doaj   +1 more source

Intraclade Heterogeneity in Nitrogen Utilization by Marine Prokaryotes Revealed Using Stable Isotope Probing Coupled with Tag Sequencing (Tag-SIP) [PDF]

Frontiers in Microbiology, 2016
Nitrogen can greatly influence the structure and productivity of microbial communities through its relative availability and form. However, the roles of specific organisms in the uptake of different nitrogen species remain poorly characterized. Most studies seeking to identify agents of assimilation have been correlative, indirectly linking activity ...
Morando, Michael, Capone, Douglas G.
openaire   +3 more sources

Multisubstrate Isotope Labeling and Metagenomic Analysis of Active Soil Bacterial Communities

mBio, 2014
Soil microbial diversity represents the largest global reservoir of novel microorganisms and enzymes. In this study, we coupled functional metagenomics and DNA stable-isotope probing (DNA-SIP) using multiple plant-derived carbon substrates and diverse ...
Y. Verastegui, J. Cheng, K. Engel, D. Kolczynski, S. Mortimer, J. Lavigne, J. Montalibet, T. Romantsov, M. Hall, B. J. McConkey, D. R. Rose, J. J. Tomashek, B. R. Scott, T. C. Charles, J. D. Neufeld   +14 more
doaj   +1 more source

Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing

Frontiers in Microbiology, 2014
Carbon cycling by microbes has been recognized as the main mechanism of organic matter decomposition and export in coastal wetlands, yet very little is known about the functional diversity of specific groups of decomposers (e.g., bacteria) in salt marsh ...
Lindsay eDarjany, Christine eWhitcraft, Jesse eDillon   +2 more
doaj   +1 more source

Determination of Resistant Starch Assimilating Bacteria in Fecal Samples of Mice by In vitro RNA-Based Stable Isotope Probing

Frontiers in Microbiology, 2017
The impact of the intestinal microbiota on human health is becoming increasingly appreciated in recent years. In consequence, and fueled by major technological advances, the composition of the intestinal microbiota in health and disease has been ...
Elena Herrmann, Wayne Young, Douglas Rosendale, Ralf Conrad, Christian U. Riedel, Markus Egert   +5 more
doaj   +1 more source

Resuscitation of the rare biosphere contributes to pulses of ecosystem activity

Frontiers in Microbiology, 2015
Dormancy is a life history trait that may have important implications for linking microbial communities to the functioning of natural and managed ecosystems.
Zach eAanderud, Stuart eJones, Noah eFierer, Jay T Lennon   +3 more
doaj   +1 more source

Genome-Resolved Proteomic Stable Isotope Probing of Soil Microbial Communities Using 13CO2 and 13C-Methanol

Frontiers in Microbiology, 2019
Stable isotope probing (SIP) enables tracking the nutrient flows from isotopically labeled substrates to specific microorganisms in microbial communities. In proteomic SIP, labeled proteins synthesized by the microbial consumers of labeled substrates are
Zhou Li, Zhou Li, Zhou Li, Qiuming Yao, Xuan Guo, Alexander Crits-Christoph, Melanie A. Mayes, William Judson Hervey IV, Sarah L. Lebeis, Jillian F. Banfield, Jillian F. Banfield, Gregory B. Hurst, Robert L. Hettich, Robert L. Hettich, Chongle Pan, Chongle Pan, Chongle Pan   +16 more
doaj   +1 more source

GLP‐1 Receptor Agonists and Endoscopy: Understanding Gastric Stasis, Aspiration Risk, and Contemporary Peri‐Procedural Guidance

Chronic Diseases and Translational Medicine, EarlyView.
Pharmacological mechanisms and physiologic effects of GLP‐1 receptor agonists relevant to peri‐endoscopic risk. ABSTRACT The global increase in obesity, type 2 diabetes mellitus (T2DM), and metabolic dysfunction‐associated steatotic liver disease (MASLD) has led to a higher use of glucagon‐like peptide‐1 receptor agonists (GLP‐1RAs) among patients ...
Junaid Beig, Jason Ta, Sophie Jones, Emily Scott, Khalid Sofi, Frances Osler, Marije Jansen, Puneet Chhabra   +7 more
wiley   +1 more source

In Vivo Isotopic Labeling of Symbiotic Bacteria Involved in Cellulose Degradation and Nitrogen Recycling within the Gut of the Forest Cockchafer (Melolontha hippocastani)

Frontiers in Microbiology, 2017
The guts of insects harbor symbiotic bacterial communities. However, due to their complexity, it is challenging to relate a specific symbiotic phylotype to its corresponding function.
Pol Alonso-Pernas, Stefan Bartram, Erika M. Arias-Cordero, Alexey L. Novoselov, Lorena Halty-deLeon, Yongqi Shao, Wilhelm Boland   +6 more
doaj   +1 more source

The plant microbiome: From ecological foundations to precision microbial engineering for sustainable agriculture

iMeta, EarlyView.
From ecological principles to precision engineering of plant microbiomes for sustainable agriculture. The plant holobiont integrates diverse microbial niches (including rhizosphere, endosphere, phyllosphere, seed, and aerial‐root mucilagesphere), host genetics (namely M genes), and host metabolites. Furthermore, molecular dialogues, including rhizobial
Mi Wei, Tengxiang Lian, Liying Chen, Lanxiang Wang, Junjie Ye, Xiaofang Yao, Shilong Duan, Ziyu Lu, Jinji Tu, Hongfu Li, Xin‐Yue Xu, Jie Zhou, Junliang He, Feiying Zhu, Paola Bonfante, Lam‐Son Phan Tran, Zhiqiang Pang, Xin Zhou, Zhihui Xu, Lin Zhang, Mengcen Wang, Xiaolin Wang, Chang‐Fu Tian, Yong‐Xin Liu, Kai Sun, Ertao Wang, Xianan Xie   +26 more
wiley   +1 more source