Results 31 to 40 of about 2,160,464 (190)
Wastewater treatment plants (WWTPs) are the main hotspots for the release of antibiotics, including the widely used sulphonamides. Microbes play important roles in eliminating sulphonamides in WWTPs, and knowledge about these degraders and their ...
Mengke Song +7 more
doaj +1 more source
SIPSim: A Modeling Toolkit to Predict Accuracy and Aid Design of DNA-SIP Experiments
DNA Stable isotope probing (DNA-SIP) is a powerful method that links identity to function within microbial communities. The combination of DNA-SIP with multiplexed high throughput DNA sequencing enables simultaneous mapping of in situ assimilation ...
Nicholas D. Youngblut +2 more
doaj +1 more source
Intraclade Heterogeneity in Nitrogen Utilization by Marine Prokaryotes Revealed Using Stable Isotope Probing Coupled with Tag Sequencing (Tag-SIP) [PDF]
Nitrogen can greatly influence the structure and productivity of microbial communities through its relative availability and form. However, the roles of specific organisms in the uptake of different nitrogen species remain poorly characterized. Most studies seeking to identify agents of assimilation have been correlative, indirectly linking activity ...
Morando, Michael, Capone, Douglas G.
openaire +3 more sources
Multisubstrate Isotope Labeling and Metagenomic Analysis of Active Soil Bacterial Communities
Soil microbial diversity represents the largest global reservoir of novel microorganisms and enzymes. In this study, we coupled functional metagenomics and DNA stable-isotope probing (DNA-SIP) using multiple plant-derived carbon substrates and diverse ...
Y. Verastegui +14 more
doaj +1 more source
Carbon cycling by microbes has been recognized as the main mechanism of organic matter decomposition and export in coastal wetlands, yet very little is known about the functional diversity of specific groups of decomposers (e.g., bacteria) in salt marsh ...
Lindsay eDarjany +2 more
doaj +1 more source
Resuscitation of the rare biosphere contributes to pulses of ecosystem activity
Dormancy is a life history trait that may have important implications for linking microbial communities to the functioning of natural and managed ecosystems.
Zach eAanderud +3 more
doaj +1 more source
: DNA/RNA-stable isotope probing (SIP) is a powerful tool to linkin situmicrobial activity to sequencing data. Every SIP dataset captures distinct information about microbial community metabolism, kinetics, and population dynamics, offering novel ...
Walls, Ramona L +6 more
core +1 more source
The impact of the intestinal microbiota on human health is becoming increasingly appreciated in recent years. In consequence, and fueled by major technological advances, the composition of the intestinal microbiota in health and disease has been ...
Elena Herrmann +5 more
doaj +1 more source
Stable Isotope Probing: Methods in Molecular Biology
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA ...
Dumont, Marc, Hernández, Marcela
core +1 more source
Methodological considerations for the use of stable isotope probing in microbial ecology
Stable isotope probing (SIP) is a method used for labeling uncultivated microorganisms in environmental samples or directly in field studies using substrate enriched with stable isotope (e.g., 13C).
Dumont, Marc +3 more
core +2 more sources

