Results 101 to 110 of about 3,617,008 (388)

A rapid staining-assisted wood sampling method for PCR-based detection of pine wood nematode Bursaphelenchus xylophilus in Pinus massoniana wood tissue [PDF]

open access: yes, 2010
For reasons of unequal distribution of more than one nematode species in wood, and limited availability of wood samples required for the PCR-based method for detecting pinewood nematodes in wood tissue of Pinus massoniana, a rapid staining-assisted wood ...
Jia, W. H.   +5 more
core   +1 more source

Mitochondrial fatty acid oxidation is stimulated by red light irradiation

open access: yesFEBS Letters, EarlyView.
Light at different wavelengths has distinct effects on keratinocyte viability and metabolism. UVA light abrogates metabolic fluxes. Blue and green light have no effect on metabolic fluxes, while red light enhanced oxidative phosphorylation by promoting fatty acid oxidation. Keratinocytes are the primary constituents of sunlight‐exposed epidermis.
Manuel Alejandro Herrera   +4 more
wiley   +1 more source

Facile fabrication of luminescent organic dots by thermolysis of citric acid in urea melt, and their use for cell staining and polyelectrolyte microcapsule labelling

open access: yesBeilstein Journal of Nanotechnology, 2016
Luminescent organic dots (O-dots) were synthesized via a one-pot, solvent-free thermolysis of citric acid in urea melt. The influence of the ratio of the precursors and the duration of the process on the properties of the O-dots was established and a ...
Nadezhda M. Zholobak   +10 more
doaj   +1 more source

Distinct expression patterns of ER alpha and ER beta in normal human mammary gland [PDF]

open access: yes, 2002
AIM: Two oestrogen receptors (ERs) have been identified to date—the “classic” ERa and the more recently described ERb. Although much is known about ERa at the mRNA and protein levels, our knowledge of the expression and distribution of ERb protein is ...
Burdall, S.E.   +3 more
core  

Quantitative Analysis of Candida Cell Wall Components by Flow Cytometrywith Triple-Fluorescence Staining [PDF]

open access: yes, 2017
This work was supported by the European Commission within the FP7 Framework Programme [Fungitect-Grant No 602125]. We also thank Thomas Sauer, Vienna Biocenter Campus (VBC), Austria, for technical support at the FACS facility of the MFPL, Karl Kuchler ...
Gow, N   +5 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

A Staining Protocol for Identifying Secondary Compounds in Myrtaceae

open access: yesApplications in Plant Sciences, 2014
Premise of the study: Here we propose a staining protocol using toluidine blue (TBO) and ruthenium red to reliably identify secondary compounds in the leaves of some species of Myrtaceae.
Hernan A. Retamales, Tanya Scharaschkin
doaj   +1 more source

Immunofluorescent Staining of Elastin

open access: yes, 2023
Figures showing specificity of elastin immunostaining procedure.
Cantor, Jerome   +3 more
openaire   +1 more source

The subnuclear localization of tRNA ligase in yeast [PDF]

open access: yes, 1987
Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
core   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

open access: yesFEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

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