Results 81 to 90 of about 3,474,741 (402)
Functional characterization of human thyroid tissue with immunohistochemistry [PDF]
Immunohistochemistry provides insights in the expression of functional proteins and of their localization in normal thyroid tissue and in thyroid diseases.
Bernard, Caillou+6 more
core +1 more source
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp+5 more
wiley +1 more source
Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue [PDF]
The ß-galactosidase gene (lacZ) of Escherichia coli is widely used as a reporter gene. The expression of lacZ can be detected by enzyme-based histochemical staining using chromogenic substrates such as 5-bromo-4-chloro-3-indolyl-ß-D: -galactoside (X-gal).
A Aguzzi+27 more
core +2 more sources
The Use of Hoechst Dyes for DNA Staining and Beyond
Hoechst dyes are among the most popular fluorophores used to stain DNA in living and fixed cells. Moreover, their high affinity and specificity towards DNA make Hoechst dyes excellent targeting moieties, which can be conjugated to various other molecules
J. Bucevičius+2 more
semanticscholar +1 more source
Brucella NyxA and NyxB dimerization enhances effector function during infection
Brucella abortus thrives inside cells thanks to the translocation of effector proteins that fine‐tune cellular functions. NyxA and NyxB are two effectors that destabilize the nucleolar localization of their host target, SENP3. We show that the Nyx proteins directly interact with each other and that their dimerization is essential for their function ...
Lison Cancade‐Veyre+4 more
wiley +1 more source
Method for copper staining of germanium crystals [PDF]
Proper conditions for copper staining of germanium crystals include a low solution temperature of 3 degrees C, illumination of the sample by infrared light, and careful positioning of the light source relative to the sample so as to minimize absorption ...
Rivet, E. J.
core +1 more source
G-banding of plant chromosomes [PDF]
A method for the staining of the chromosomal material of plant cells so as to induce G-Bands, useful in plant genetic studies such as hydridization. G-Banding is induced by contacting living plant cells, such as those of plants of the family Gramineae ...
Hsu, Tao-Chiuh
core +1 more source
UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg+2 more
wiley +1 more source
Presence and localization of a 30-kDa basic fibroblast growth factor-like protein in rodent testes [PDF]
We have used a recently characterized rabbit antiserum against basic fibroblast growth factor (bFGF), which recognizes various forms of bFGF, to examine the presence and localization of bFGF in the testes of adult rats and mice and the 5-day-old rat.
Gratzl, Manfred+4 more
core +1 more source
The stain asserts its autonomy as a spot or patch of colour different from the ground. It exists in and of itself. The stain tells us what it means to be the medium of visibility. Hence, every stain is a Metabild, a particular image that explains something about the image as a phenomenon. These properties make the stain a paradigm for the visual medium
openaire +1 more source