Results 11 to 20 of about 478,710 (331)

Rapid and Uniform NHS-Ester-Based Membrane Protein Labeling of Live Mammalian Cells [PDF]

Bio-Protocol
Rapid and uniform labeling of plasma membrane proteins is essential for high-resolution imaging of dynamic membrane topologies and intercellular communication in live mammalian cells.
Alyssa Burgess, Hirushi Gunasekara, Ying Hu   +2 more
doaj   +2 more sources

Virtual Gram staining of label-free bacteria using dark-field microscopy and deep learning [PDF]

Science Advances
Gram staining has been a frequently used staining protocol in microbiology. It is vulnerable to staining artifacts due to, e.g., operator errors and chemical variations. Here, we introduce virtual Gram staining of label-free bacteria using a trained neural network that digitally transforms dark-field images of unstained bacteria into their
Çağatay Işıl, Hatice Ceylan Koydemir, Merve Eryılmaz, Kevin de Haan, Nir Pillar, Koray Mentesoglu, Aras Firat Unal, Yair Rivenson, Sukantha Chandrasekaran, Omai B. Garner, Aydogan Özcan   +10 more
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Copper Staining/Labeling and Scanning Electrochemical Microscopy Readout of Proteins on Poly(vinylidene difluoride) Membranes

CHIMIA, 2005
Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize roteins on surfaces. Proteins are adsorbed on a poly(vinylidene difluoride) (PVDF) membrane and stained using a standard protocol involving ...
Maurizio Carano, Niels Lion, Hubert H. Girault   +2 more
doaj   +4 more sources

Virtual Staining, Segmentation, and Classification of Blood Smears for Label-Free Hematology Analysis [PDF]

BME Frontiers, 2022
Objective and Impact Statement . We present a fully automated hematological analysis framework based on single-channel (single-wavelength), label-free deep-ultraviolet (UV) microscopy that serves as a fast, cost-effective alternative to conventional hematology analyzers. Introduction .
Nischita Kaza, Ashkan Ojaghi, Francisco E. Robles   +2 more
openalex   +4 more sources

Basophilic peripheral nerve inclusions in a patient with L144F SOD1 amyotrophic lateral sclerosis [PDF]

Vojnosanitetski Pregled, 2023
Introduction. Histopathological findings of various inclusions were reported in the central nervous system of amyotrophic lateral sclerosis (ALS) patients but not in the peripheral nerves. Case report.
Aleksić Dejan, Perić Stojan, Milenković Sanja, Janković Milena, Rakočević-Stojanović Vidosava, Stević Zorica   +5 more
doaj   +1 more source

Semi-Automatic Labeling and Semantic Segmentation of Gram-Stained Microscopic Images from DIBaS Dataset [PDF]

2023 2nd International Conference on Computational Systems and Communication (ICCSC), 2022
In this paper, a semi-automatic annotation of bacteria genera and species from DIBaS dataset is implemented using clustering and thresholding algorithms. A Deep learning model is trained to achieve the semantic segmentation and classification of the bacteria species. Classification accuracy of 95% is achieved.
Chethan Reddy G. P., Pullagurla Abhijith Reddy, Vidyashree R. Kanabur, Deepu Vijayasenan, S. Sumam David, Sreejith Govindan   +5 more
openalex   +3 more sources

Melanin-Binding Colorants: Updating Molecular Modeling, Staining and Labeling Mechanisms, and Biomedical Perspectives [PDF]

Colorants, 2022
Melanin and melanoma tumors are two fields of increasing interest in biomedical research. Melanins are ubiquitous biopigments with adaptive value and multiple functions, and occur in the malignant melanoma. Although several chemical structures have been proposed for eumelanin, molecular modeling and orbitals indicate that a planar or spiral ...
Juan C. Stockert, Jesús Espada, Alfonso Blázquez-Castro   +2 more
openaire   +3 more sources

Comparison of β-Amyloid Plaque Labeling Methods: Antibody Staining, Gallyas Silver Staining, and Thioflavin-S Staining [PDF]

Chinese Medical Sciences Journal, 2018
Objective To evaluate senile plaque formation and compare the sensitivity of three different β-amyloid (Aβ) labeling methods (antibody staining, Gallyas silver staining, and thioflavin-S staining) to detect Aβ deposition.Methods APPswe/PSEN1dE9 transgenic mice (APP/PS1) of different ages were used to examine spatiotemporal changes in Aβ plaque ...
Xin-Ze, Shi, Xuan, Wei, Long-Ze, Sha, Qi, Xu   +3 more
openaire   +2 more sources

Epigenetics and cell death: DNA hypermethylation in programmed retinal cell death. [PDF]

, 2013
BackgroundVertebrate genomes undergo epigenetic reprogramming during development and disease. Emerging evidence suggests that DNA methylation plays a key role in cell fate determination in the retina.
Enke, Raymond A, Fuller, John A, Kalesnykas, Giedrius, Merbs, Shannath L, Wahlin, Karl J, Zack, Donald J   +5 more
core   +15 more sources

Metastatic Dedifferentiated Chondrosarcoma to Thyroid Gland: A Case Report

Oman Medical Journal, 2021
A 54-year-old man presented with a pathologic hip fracture secondary to a right femur tumor. A reaming biopsy showed the diagnosis of dedifferentiated chondrosarcoma (DDC). In addition, the patient had a thyroid mass.
Tariq N. Aladily , Nisreen Abushahin, Randa Mahgoub, Bassem I. Haddad   +3 more
doaj   +1 more source