Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]
BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F.+2 more
core +1 more source
Multicolour lineage tracing reveals clonal dynamics of squamous carcinoma evolution from initiation to metastasis. [PDF]
Tumour cells are subjected to evolutionary selection pressures during progression from initiation to metastasis. We analysed the clonal evolution of squamous skin carcinomas induced by DMBA/TPA treatment using the K5CreER-Confetti mouse and stage ...
Balmain, Allan+4 more
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Chromosome Centromeres: Structural and Analytical Investigations with High Resolution Scanning Electron Microscopy in Combination with Focused Ion Beam Milling [PDF]
Whole mount mitotic metaphase chromosomes of different plants and animals were investigated with high resolution field emission scanning electron microscopy (FESEM) to study the ultrastructural organization of centromeres, including metacentric ...
Schroeder-Reiter, E., Wanner, Gerhard
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Specific fluorescent labeling of chicken myofibril Z-line proteins catalyzed by guinea pig liver transglutaminase [PDF]
Guinea pig liver transglutaminase has been found to catalyze the covalent incorporation of dansylcadaverine into chicken skeletal muscle myofibril proteins.
Gard, David L., Lazarides, Elias
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Tetanic Stimulation Leads to Increased Accumulation of Ca^(2+)/Calmodulin-Dependent Protein Kinase II via Dendritic Protein Synthesis in Hippocampal Neurons [PDF]
mRNA for the ɑ-subunit of CaMKII is abundant in dendrites of neurons in the forebrain (Steward, 1997). Here we show that tetanic stimulation of the Schaffer collateral pathway causes an increase in the concentration of ɑ-CaMKII in the dendrites of ...
Kennedy, Mary B.+4 more
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In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]
We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Acharya, Chitrangada+9 more
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Structural and molecular characteristics of axons in the long head of the biceps tendon [PDF]
The innervation of the long head of the biceps tendon (LHBT) is not sufficiently documented. This is a drawback since pathologies of the LHBT are a major source of shoulder pain.
Blumer, Roland+7 more
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Axons Giving Rise to the Palisade Endings of Feline Extraocular Muscles Display Motor Features [PDF]
Palisade endings are nerve specializations found in the extraocular muscles (EOMs) of mammals, including primates. They have long been postulated to be proprioceptors. It was recently demonstrated that palisade endings are cholinergic and that in monkeys
Davis López de Carrizosa, María América+5 more
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The structural organization and protein composition of lens fiber junctions. [PDF]
The structural organization and protein composition of lens fiber junctions isolated from adult bovine and calf lenses were studied using combined electron microscopy, immunolocalization with monoclonal and polyclonal anti-MIP and anti-MP70 (two putative
Ehring, GR+3 more
core +2 more sources
Identification of novel small molecule inhibitors of ETS transcription factors
ETS transcription factors play an essential role in tumourigenesis and are indispensable for sprouting angiogenesis, a hallmark of cancer, which fuels tumour expansion and dissemination. Thus, targeting ETS transcription factor function could represent an effective, multifaceted strategy to block tumour growth. The evolutionarily conserved E‐Twenty‐Six
Shaima Abdalla+9 more
wiley +1 more source