Results 161 to 170 of about 28,825 (215)

Guanidination and nitroguanidination of staphylococcal enterotoxin B

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1971
Guanidination of the free amino groups of staphylococcal enterotoxin B with 3,5-dimethyl-1-guanylpyrazole converted 31-32 of 33 epsilon-amino groups and 30% of the N-terminal residue. This product, although markedly reduced in solubility, suffered no gross change in conformation and retained full biological activity.
L, Spero, H M, Jacoby, J E, Dalidowicz, S J, Silverman   +3 more
openaire   +2 more sources

Analytical platform with selectable assay parameters based on three functions of magnetic nanoparticles: demonstration of high-ly sensitive rapid quantitation of staphylococcal enterotoxin B in food.

Analytical Chemistry, 2019
Many immunoassay platforms require time- and labor-consuming tuning of parameters for operation in complex mediums (food, whole blood, etc.), but no universal method has been proposed to accelerate that "trial-and-error" stage.
V. A. Bragina, S. L. Znoyko, A. Orlov, A. V. Pushkarev, M. Nikitin, P. Nikitin   +5 more
semanticscholar   +1 more source

Ultrasensitive detection of staphylococcal enterotoxin B in foodstuff through dual signal amplification by bio-barcode and real-time PCR.

Food Chemistry, 2019
This study developed an ultrasensitive method involving (1) gold nanoparticles encoded with a double-stranded DNA as the first signal amplification and goat anti-staphylococcal enterotoxin B (SEB) polyclonal antibody and (2) magnetic microparticles ...
Yanyang Xu, Bingyang Huo, C. Li, Yuan Peng, S. Tian, Longxing Fan, Jialei Bai, Baoan Ning, Zhixian Gao   +8 more
semanticscholar   +1 more source

Raising Antibodies to Staphylococcal Enterotoxins A and B

Journal of Applied Bacteriology, 1977
A method is described for raising specific antibodies to staphylococcal enterotoxins A (SEA) and B (SEB) by intravenous inoculation of rabbits with small doses of enterotoxin diluted in sterile physiological saline. A course of six injections over a period of two weeks was given on four occasions.
C M, Bradstreet, M W, Dighero, R J, Gilbert, A A, Wieneke   +3 more
openaire   +2 more sources

Magnetic-plasmonic blackbody enhanced lateral flow immunoassay of staphylococcal enterotoxin B.

Food Chemistry
Staphylococcal enterotoxin B (SEB) in food is a serious health risk, making rapid and accurate detection methods essential. Herein, we synthesized a magnetic plasmonic blackbody, Fe3O4@Au/PDA, by coating a gold/polydopamine (Au/PDA) layer onto an Fe3O4 ...
Jiayi Sun, Yuhao Wu, Xinya Fan, Juan Peng, Xiaolong Wang, Y. Xiong, Xiaolin Huang   +6 more
semanticscholar   +1 more source

Mesenchymal stem cells preconditioned by staphylococcal enterotoxin B enhance survival and bacterial clearance in murine sepsis model.

Cytotherapy, 2019
Sepsis, a health-threatening progressive infectious disease, is the major cause of morbidity and mortality worldwide. Cell therapy using mesenchymal stromal cells (MSCs) is an innovative strategy with excessive therapeutic potential in the treatment of ...
Pardis Saeedi, R. Halabian, A. I. Fooladi   +2 more
semanticscholar   +1 more source

AlphaLISA for detection of staphylococcal enterotoxin B free from interference by protein A.

Toxicon, 2019
Staphylococcal enterotoxin B (SEB) is an important enterotoxin which is a major reason for food poisoning and a potential biologic agent. Hence, rapid and accurate detection is very important.
Junqing Zhao, Qingyu Lv, Peng Liu, Liyuan Guo, Liwen Zhang, Yuling Zheng, Lihua Ming, Decong Kong, Hua Jiang, Yongqiang Jiang   +9 more
semanticscholar   +1 more source

Immunosuppression induced by staphylococcal enterotoxin B

Cellular Immunology, 1982
Abstract Staphylococcal enterotoxin B (SEB) is a potent mitogen for both human and murine T lymphocytes. We report here studies which demonstrate that a suppressor cell population, capable of suppressing the primary immune response of normal syngeneic mouse splenocytes to heterologous sheep erythrocytes (SRBC), is activated by SEB.
Donnelly, R P, Rogers, T J
openaire   +2 more sources

A staphylococcal enterotoxin B magnetoelastic immunosensor

Biosensors and Bioelectronics, 2004
A magnetoelastic immunosensor for detection of staphylococcal enterotoxin B (SEB) is described. The magnetoelastic sensor is a newly developed mass/elasticity-based transducer of high sensitivity having a material cost of approximately $0.001/sensor.
Chuanmin, Ruan, Kefeng, Zeng, Oomman K, Varghese, Craig A, Grimes   +3 more
openaire   +2 more sources

Binding of flavonoids to staphylococcal enterotoxin B

Food and Chemical Toxicology, 2014
Staphylococcal enterotoxins are metabolic products of Staphylococcus aureus that are responsible for the second-most-commonly reported type of food poisoning. Polyphenols are known to interact with proteins to form complexes, the properties of which depend on the structures of both the polyphenols and the protein.
Evgen Benedik, Mihaela Skrt, Črtomir Podlipnik, Nataša Poklar Ulrih   +3 more
openaire   +2 more sources