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Fabricating and regulating peroxidase-like activity of eggshell membrane-templated gold nanoclusters for colorimetric detection of staphylococcal enterotoxin B.

Talanta: The International Journal of Pure and Applied Analytical Chemistry, 2019
Fluorescent eggshell membrane-templated gold nanoclusters (Au-ESM) can be obtained in a facile and low-cost manner in this study. The fluorescence of the Au-ESM may be significantly quenched by mercapto-compounds and peroxidase-like activity of Au-ESM ...
Fang Tan   +6 more
semanticscholar   +1 more source

Microheterogeneity of staphylococcal enterotoxin B

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1974
Abstract Four components have been demonstrated in staphylococcal enterotoxin B by isoelectric focusing in polyacrylamide gels. In a typical preparation their relative concentrations from the most to least cathodic were 6, 56, 31 and 7%. The components were not stable conformers nor were the differences in isoionic points due to bound ligand.
Leonard Spero   +2 more
openaire   +1 more source

The secondary structure of staphylococcal enterotoxins A, B and C

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1980
The circular dichroism (CD) of staphylococcal enterotoxins A, B and C was measured. The CD of enterotoxins B and C were almost identical from 250 to 320 nm, but differed from the CD of enterotoxin A. The spectrum of enterotoxin A in this wavelength region contained the same bands with respect to both location and sign, but with significant differences ...
J L, Middlebrook, L, Spero, P, Argos
openaire   +2 more sources

Staphylococcal Enterotoxin B

2003
Toxins are biologically derived substances that adversely affect living organisms. An astounding number of toxins produced by animals, plants, and bacteria are harmful to humans. Toxins cause food poisonings and envenomations, bleeding disorders, and neurological dysfunction.
openaire   +1 more source

On the Cross-Reactivity of Staphylococcal Enterotoxins A, B, and C

The Journal of Immunology, 1978
Abstract Strong cross-reactions were demonstrated for staphylococcal enterotoxins B (SEB) and C1 (SEC1) by antigenbinding capacity and by competitive binding ability. Both SEB and SEC1 combined completely with the heterologous antibody although requiring four times as much antiserum as the homologous enterotoxin and both displaced about ...
L, Spero, B A, Morlock, J F, Metzger
openaire   +2 more sources

Double-antibody radioimmunoassay for staphylococcal enterotoxins A and B

Canadian Journal of Microbiology, 1978
A sensitive double-antibody radioimmunoassay for staphylococcal enterotoxins A and B is described. The separation of the primary antigen–antibody complex of enterotoxin A and B was achieved with an anti-rabbit gamma globulin from goats. Radioiodinated aggregate fractions of staphylococcal enterotoxins exhibited reduced immunological activity and ...
H, Robern, T M, Gleeson, R A, Szabo
openaire   +2 more sources

Antigenicity of Formaldehyde-Inactivated Staphylococcal Enterotoxin B

The Journal of Immunology, 1973
Abstract The reactivity toward or ability to stimulate production of antibodies specific for native staphylococcal enterotoxin B (nSEB) demonstrated by formaldehyde-inactivated toxin (fSEB) is directly related to the extent to which SEB is polymerized by aqueous formaldehyde.
J R, Warren, L, Spero, J F, Metzger
openaire   +2 more sources

Fractionation of staphylococcal enterotoxin B by isoelectric focusing

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1971
Abstract 1. 1. In the context of studies concerned with a radioimmunoassay procedure for detection of staphylococcal enterotoxins in food, enterotoxin B was purified from the culture supernatant of Staphylococcus aureus, strain S-6. Purified preparations were subjected to the technique of isoelectric focusing of proteins in a stable pH gradient ...
P C, Chang, N, Dickie
openaire   +2 more sources

A capacitive biosensor for detection of staphylococcal enterotoxin B

Analytical and Bioanalytical Chemistry, 2008
A sensitive method for the detection of staphylococcal enterotoxin B (SEB) using a flow-injection capacitive biosensor is presented. SEB was purified from a crude culture filtrate of Staphylococcus aureus through three chromatographic steps. The first two steps were based on ion-exchange chromatography, and the last step was carried out on a gel ...
Mahmoud, Labib   +3 more
openaire   +2 more sources

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