Results 41 to 50 of about 20,557 (265)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

New evidence of binarity in young α-rich turn-off and subgiant stars: fast rotation and strong magnetic activity [PDF]

open access: yes
Young α-rich (YAR) stars within the old Galactic thick disc exhibit a dual characteristic of relative youth determined with asteroseismology and abundance enhancement in α elements measured from high-resolution spectroscopy. The youth origin of YAR stars
Murphy, Simon J.   +5 more
core   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

The 2D dynamics of radiative zones of low-mass stars

open access: yes, 2018
Context. Helioseismology and asteroseismology allow us to probe the differential rotation deep within low-mass stars. In the solar convective envelope, the rotation varies with latitude with an equator rotating faster than the pole, which results in a ...
Mathis, Stéphane   +5 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Bayesian Ages for Early-type Stars from Isochrones Including Rotation, and a Possible Old Age for the Hyades

open access: yes, 2015
We combine recently computed models of stellar evolution using a new treatment of rotation with a Bayesian statistical framework to constrain the ages and other properties of early-type stars. We find good agreement for early-type stars and clusters with
Huang, Chelsea X., Brandt, Timothy D.
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

New measurements of rotation and differential rotation in A-F stars: are there two populations of differentially rotating stars? [PDF]

open access: yesAstronomy & Astrophysics, 2012
16 pages, 13 figures, 4 tables, accepted for publication in A& ...
Ammler-von Eiff, M., Reiners, Ansgar
openaire   +4 more sources

Rotation-Activity-Age Relations for Solar-Type and Cooler Stars

open access: yes, 2016
The fact that stellar rotation and chromospheric emission are correlated with age was explicitly noted by Wilson (1963) and reinforced by Kraft (1967). Wilson knew that Ca II emission was correlated with surface magnetic field in the Sun. Skumanich (1972)
Basri, Gibor, Basri, Gibor (5201506)
core   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

open access: yesFEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel   +8 more
wiley   +1 more source

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