Results 81 to 90 of about 25,057 (234)

Extracellular Vesicles‐Based Biological Macromolecule Delivery Systems: Biogenesis, Characterization, and Emerging Clinical Applications

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Extracellular vesicles (EVs) are nanoscale membrane‐bound structures that play a pivotal role in intercellular communication by transporting bioactive molecules, including proteins, lipids, nucleic acids, and organelles, between cells. Originating from the endosomal pathway, EVs reflect the physiological and pathological status of their parent
Emine İncilay Torunoğlu, Zeynep Betül Sarı, Muhammed Emin Sarı, Erdi Can Aytar   +3 more
wiley   +1 more source

Resolution scaling in STED microscopy

Optics Express, 2008
We undertake a comprehensive study of the inverse square root dependence of spatial resolution on the saturation factor in stimulated emission depletion (STED) microscopy and generalize it to account for various focal depletion patterns. We used an experimental platform featuring a high quality depletion pattern which results in operation close to the ...
Harke, Benjamin, Keller, Jan, Ullal, Chaitanya K., Westphal, Volker, Schönle, Andreas, Hell, Stefan   +5 more
openaire   +4 more sources

STED nanoscopy with fluorescent quantum dots. [PDF]

, 2015
The widely popular class of quantum-dot molecular labels could so far not be utilized as standard fluorescent probes in STED (stimulated emission depletion) nanoscopy.
Hoyer, Patrick, Falk, H., Görlitz, F., Görlitz, Frederik, Hanne, Janina, Engelhardt, Johann, Hanne, J., Falk, Henning J., Hoyer, P., Engelhardt, J., Hell, Stefan, Sahl, Steffen J., Sahl, S., Hell, S. ; https://orcid.org/   +13 more
core   +1 more source

Aberration correction for improving the image quality in STED microscopy using the genetic algorithm

Nanophotonics, 2018
With a purely optical modulation of fluorescent behaviors, stimulated emission depletion (STED) microscopy allows for far-field imaging with a diffraction-unlimited resolution in theory.
Wang Luwei, Yan Wei, Li Runze, Weng Xiaoyu, Zhang Jia, Yang Zhigang, Liu Liwei, Ye Tong, Qu Junle   +8 more
doaj   +1 more source

Bicolor Bessel beams generator based on single-mode fiber for STED system light source [PDF]

EPJ Web of Conferences
In this paper, we have presented a novel and compact bicolor Bessel beams (bi-BBs) generator based on a single-mode fiber integrated with an axicon-like microstructure (AM).
Shao Luqing, Li Xijun, Qiu Min
doaj   +1 more source

Electron compensation‐gated nanomotor for immunomodulation and microenvironment remodeling in bacterial pneumonia therapy

BMEMat, EarlyView.
A nanomotor featuring an “electronic lock” via electron compensation in an Au‐Ag alloy ensures therapeutic precision. Near‐infrared light acts as a key, unlocking on‐demand Ag+ release for synergistic bactericidal action and microenvironment remodeling to potently treat bacterial pneumonia. Abstract Bacterial pneumonia treatment is severely hampered by
Chenglong Xue, Xiaoyu Zhao, Yuqian Wang, Lijin Shi, Qi Shuai   +4 more
wiley   +1 more source

STED-SPIM made simple

Optics Express, 2014
We report the development of a stimulated emission depletion (STED) selective plane illumination (SPIM) microscope based on a single diode-pumped solid state (DPSS) laser that simultaneously delivers nanosecond-pulses at two wavelengths. The two wavelengths, 355 nm and 532 nm, are generated by harmonic conversion and they are used to induce ...
Scheul, Teodora, Wang, Irène, Vial, Jean-Claude, Aimé   +2 more
openaire   +3 more sources

RhodaMal Dyes: Novel Red Emissive Fluorophores as Probes for High Contrast Live‐Cell Imaging

Chemistry – A European Journal, EarlyView.
Incorporation of malononitrile into the traditional rhodol framework furnishes RhodaMal (Rhoda = Rhodamine, Mal = Malononitrile;) dyes, a new class of red emissive push–pull chromophores. Conjugating them to triphenylphosphonium cation enables live cell super‐resolution STED microscopy with a 775 nm depletion laser.
A. Mavroskoufis, P. Aßmann, L. M. Filla, I. Bilici, E. Quaas, M. Maglione, S. Sigrist, R. Haag, K. Licha   +8 more
wiley   +1 more source

Nanoparticle-assisted STED nanoscopy with gold nanospheres [PDF]

, 2017
We demonstrate stimulated emission depletion (STED) microscopy with 20 nm gold nanospheres coated by fluorescent silica. Compared with previous demonstrations of STED with a hybrid plasmonic fluorescent label, the current implementation offers a ...
Sivan, Yonatan, Sivan, Y., Hell, Stefan W., Urban, Nicolai T., Foreman, Matthew, Yonatan Sivan, Foreman, Matthew R, Urban, N., Matthew R. Foreman, Urban, Nicolai T, Hell, Stefan W, Foreman, M., Nicolai T. Urban, Stefan W. Hell, Hell, S. ; https://orcid.org/   +14 more
core   +1 more source

Assembly and Maintenance of Myofibrils in the Heart

Cytoskeleton, EarlyView.
ABSTRACT The assembly of actin and myosin to the paracrystalline arrangement of myofibrils in striated muscle, that is, heart and skeletal, has puzzled many a cell biologist. Different models, based on cultured cardiomyocytes, were proposed for the last 30 years to explain the sequence of events.
Elisabeth Ehler
wiley   +1 more source