Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases [PDF]
Biotechnology for Biofuels, 2017 Background Bioethanol production from sustainable sources of biomass that limit effect on food production are needed and in a biorefinery approach co-products are desirable, obtained from both the plant material and from the microbial biomass.
Claire M. Hull +6 more
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Unlocking Testosterone Production by Biotransformation: Engineering a Fungal Model of Aspergillus nidulans Strain Deficient in Steroid 11α-Hydroxylase Activity and Expressing 17β-Hydroxysteroid Dehydrogenase Enzyme as Proof of Concept [PDF]
BiomoleculesTestosterone holds significant medical and economic importance, with the global market for testosterone replacement therapies valued at approximately USD 1.9 billion in 2023.
Lidia Ortega-de los Ríos +6 more
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Bacterial steroid hydroxylases: enzyme classes, their functions and comparison of their catalytic mechanisms. [PDF]
Appl Microbiol Biotechnol, 2018 The steroid superfamily includes a wide range of compounds that are essential for living organisms of the animal and plant kingdoms. Structural modifications of steroids highly affect their biological activity.
Szaleniec M +4 more
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Minor activities and transition state properties of the human steroid hydroxylases cytochromes P450c17 and P450c21, from reactions observed with deuterium-labeled substrates. [PDF]
Biochemistry, 2012 The steroid hydroxylases CYP17A1 (P450c17, 17-hydroxylase/17,20-lyase) and CYP21A2 (P450c21, 21-hydroxylase) catalyze progesterone hydroxylation at one or more sites within a 2 Å radius.
Yoshimoto FK +7 more
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Bioconversion of vitamin D3 to bioactive calcifediol and calcitriol as high-value compounds [PDF]
Biotechnology for Biofuels and Bioproducts, 2022 Biological catalysis is an important approach for the production of high-value-added compounds, especially for products with complex structures. Limited by the complex steps of chemical synthesis and low yields, the bioconversion of vitamin D3 (VD3) to ...
Zheyi Wang +6 more
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Am J Physiol Endocrinol Metab, 2011 The regulation of cortisol synthesis and the expression of genes coding for steroidogenic proteins by 8-substituted cAMP and 8-substituted adenine derivatives were studied in bovine adrenal zona fasciculata (AZF) cells.
Enyeart JA, Liu H, Enyeart JJ.
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An Inhibitor of Adrenal Steroid 11β-Hydroxylase [PDF]
Nature, 1964 Trans-1,4-bis (2-chlorobenzylaminomethyl) cyclohexane dihydrochloride (‘AY–9944’)1 has been shown to represent a novel class of cholesterol biosynthesis inhibitors which act by interfering with the enzymatic conversion of 7-dehydrocholesterol to cholesterol2,3. In laboratory animals, ‘AY–9944’ significantly depressed serum sterol-levels4.
Morris L. Givner +3 more
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Crystal Structure and Biochemical Analysis of a Cytochrome P450 Steroid Hydroxylase (BaCYP106A6) from Bacillus Species. [PDF]
J Microbiol Biotechnol, 2023 Cytochrome P450 (CYP) is a heme-containing enzyme that catalyzes hydroxylation reactions with various substrate molecules. Steroid hydroxylases are particularly useful for effectively introducing hydroxyl groups into a wide range of steroids in the ...
Kim KH +7 more
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Studies on Adrenal Steroid Hydroxylases
Journal of Biological Chemistry, 1973 Abstract Adrenodoxin reductase and adrenodoxin have been shown to form a complex with a stoichiometric molar ratio of 1:1. The dissociation constant of the complex is found to be approximately 10-9 m in a low ionic strength medium, and approximately 10-6 m in a high ionic strength medium.
Jau‐Wen Chu, Tokuji Kimura
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Synthesis of halogenated pregnanes, mechanistic probes of steroid hydroxylases CYP17A1 and CYP21A2. [PDF]
J Steroid Biochem Mol Biol, 2012 Yoshimoto FK, Desilets MC, Auchus RJ.
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