Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases [PDF]
Biotechnology for Biofuels, 2017 Background Bioethanol production from sustainable sources of biomass that limit effect on food production are needed and in a biorefinery approach co-products are desirable, obtained from both the plant material and from the microbial biomass.
Claire M. Hull +6 more
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The curious family of cytochrome P450 4F fatty acid ω-hydroxylases: recent developments in their function and relevance for human health [PDF]
Open BiologyCytochrome P450 enzymes (CYP, P450) are indispensable for human health and catalyse a plethora of reactions contributing to the clearance of xenobiotics, steroid and bile acid biosynthesis, and the functionalization of vitamins in the human body.
Brisa Caroline Alves Chagas +3 more
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Unlocking Testosterone Production by Biotransformation: Engineering a Fungal Model of Aspergillus nidulans Strain Deficient in Steroid 11α-Hydroxylase Activity and Expressing 17β-Hydroxysteroid Dehydrogenase Enzyme as Proof of Concept [PDF]
BiomoleculesTestosterone holds significant medical and economic importance, with the global market for testosterone replacement therapies valued at approximately USD 1.9 billion in 2023.
Lidia Ortega-de los Ríos +6 more
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Bioconversion of vitamin D3 to bioactive calcifediol and calcitriol as high-value compounds [PDF]
Biotechnology for Biofuels and Bioproducts, 2022 Biological catalysis is an important approach for the production of high-value-added compounds, especially for products with complex structures. Limited by the complex steps of chemical synthesis and low yields, the bioconversion of vitamin D3 (VD3) to ...
Zheyi Wang +6 more
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Functional characterization and unraveling the structural determinants of novel steroid hydroxylase CYP154C7 from Streptomyces sp. PAMC26508 [PDF]
HeliyonThis study characterized cytochrome P450 enzyme CYP154C7 from Streptomyces sp. PAMC26508, emphasizing its capability to hydroxylate steroids, especially at the 16α-position.
Prakash Paudel +4 more
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An Inhibitor of Adrenal Steroid 11β-Hydroxylase [PDF]
Nature, 1964 Trans-1,4-bis (2-chlorobenzylaminomethyl) cyclohexane dihydrochloride (‘AY–9944’)1 has been shown to represent a novel class of cholesterol biosynthesis inhibitors which act by interfering with the enzymatic conversion of 7-dehydrocholesterol to cholesterol2,3. In laboratory animals, ‘AY–9944’ significantly depressed serum sterol-levels4.
Morris L. Givner +3 more
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Correction to: Bacterial steroid hydroxylases: enzyme classes, their functions and comparison of their catalytic mechanisms. [PDF]
Appl Microbiol Biotechnol, 2018 Szaleniec M +4 more
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Metabolites of an Epac-selective cAMP analog induce cortisol synthesis by adrenocortical cells through a cAMP-independent pathway. [PDF]
PLoS ONE, 2009 Adrenal zona fasciculata (AZF) cells express a cAMP-activated guanine nucleotide exchange protein (Epac2) that may function in ACTH-stimulated cortisol synthesis.
Judith A Enyeart, John J Enyeart
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Flavoprotein monooxygenases for oxidative biocatalysis: recombinant expression in microbial hosts and applications [PDF]
, 2014 External flavoprotein monooxygenases comprise a group of flavin-dependent oxidoreductases that catalyze the insertion of one atom of molecular oxygen into an organic substrate and the second atom is reduced to water. These enzymes are involved in a great
Bianchi, Dario Alejandro +2 more
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mBio, 2018 Side chain-containing steroids are ubiquitous constituents of biological membranes that are persistent to biodegradation. Aerobic, steroid-degrading bacteria employ oxygenases for isoprenoid side chain and tetracyclic steran ring cleavage. In contrast, a
Christian Jacoby +10 more
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