Results 151 to 160 of about 3,096 (185)

Steroid 21-Hydroxylase

1993
Cortisol synthesis in the zona fasciculata of the adrenal cortex requires five enzymatic steps: the cholesterol side chain is cleaved to yield pregnenolone, which is dehydrogenated at the 3β position to progesterone, which is successively hydroxylated at the 17α, 21 and 11β positions. The side-chain cleavage and the three hydroxylations are mediated by
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A new ELISA for autoantibodies to steroid 21-hydroxylase

Clinical Chemistry and Laboratory Medicine (CCLM), 2017
Abstract Background: A new ELISA for autoantibodies to steroid 21-hydroxylase (21-OH Ab) is described. Methods: In the assay test sample autoantibodies form a bridge between 21-OH coated onto the plate well and liquid phase 21-OH ...
Maria, Del Pilar Larosa, Shu, Chen, Nora, Steinmaus, Hannah, Macrae, Liang, Guo, Stefano, Masiero, Silvia, Garelli, Miriam Dalla, Costa, Artur, Bossowski, Jadwiga, Furmaniak, Corrado, Betterle, Bernard Rees, Smith   +11 more
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Bovine steroid 21-hydroxylase: regulation of biosynthesis

Biochemistry, 1986
A recombinant cDNA clone, PBC21-1, specific for bovine steroid 21-hydroxylase cytochrome P-450 (P-450C21) was identified in a bovine adrenocortical cDNA library, and this identity was confirmed by nucleotide sequencing which revealed significant amino acid homology (77%) with human P-450C21 cDNA.
M E, John, T, Okamura, A, Dee, B, Adler, M C, John, P C, White, E R, Simpson, M R, Waterman   +7 more
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Molecular pathology of steroid 21-hydroxylase deficiency

The Journal of Steroid Biochemistry and Molecular Biology, 1991
The molecular pathology of steroid 21-hydroxylase deficiency is attributable to unequal crossover-mediated gene deletion or to large- or small-scale replacement of the functional CYP21B gene sequence by a copy of the analogous CYP21A pseudogene sequence.
T, Strachan, P C, White
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Factors influencing steroid hydroxylases in liver microsomes

Advances in Enzyme Regulation, 1966
Abstract Liver microsomal enzymes oxidatively metabolize drugs, insecticides and steroids in the presence of TPNH and oxygen to compounds that are more polar than the substrate. Several factors listed below which alter the activity of the oxidative drug-metabolizing enzymes in liver microsomes, similarly alter the activity of liver microsomal enzymes
R, Kuntzman, R, Welch, A H, Conney
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Genetics of Adrenal Steroid 21-Hydroxylase Deficiency*

Endocrine Reviews, 1986
Impairment of 21-hydroxylation is the most common enzymatic deficiency resulting in the syndrome of CAH, which may present either in the classical form in infants or in the nonclassical form in older individuals. Variable signs and symptoms of androgen excess are common to both types of the disorder, which are transmitted as autosomal recessive traits ...
M I, New, P W, Speiser
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Extraadrenal Steroid 21-Hydroxylase Activity in a Woman with Congenital Adrenal Hyperplasia due to Steroid 21-Hydroxylase Deficiency*

The Journal of Clinical Endocrinology & Metabolism, 1983
The 21-hydroxylation of plasma progesterone (P) has been demonstrated in pregnant, nonpregnant, and adrenalectomized women and in men. The fractional conversion of plasma progesterone to deoxycorticosterone (DOC), [rho]P-DOC BU, among those subjects was 0.009 +/- 0.001 (mean +/- SEM, n = 32).
C A, Winkel, M L, Casey, R J, Worley, J D, Madden, P C, MacDonald   +4 more
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Urinary steroid excretion in 17α-hydroxylase deficiency

Journal of Steroid Biochemistry, 1978
Abstract Urinary steroids from a patient with 17α-hydroxylase deficiency syndrome have been identified. The steroids were obtained by enzymatic hydrolysis of urine, Amberlite XAD-2 extraction and Sephadex LH-20 column chromatography. Following preparation of volatile derivatives, the steroids were separated and estimated by gas chromatography on open-
J W, Honour, J, Tourniaire, E G, Biglieri, C H, Shackleton   +3 more
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Steroid 16α‐Hydroxylase from Human Fetal Liver; Inhibition by Steroids

Acta Obstetricia et Gynecologica Scandinavica, 1980
The 16α‐hydroxylase system in fetal liver which used dehydroepiandrosterone (DHA) or pregnenolone as substrate, was apparently inhibited by various endogenous and synthetic steroids: DHA, pregnenolone, their sulfates, androstenediol, androstenetriol, estrone, estradiol‐17β, ethynylestradiol and chlormadinone‐acetate.
Y, Sano, H, Shibusawa, N, Yoshida, K, Sekiba, S, Okinaga, K, Arai   +5 more
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Disorders of the Aldosterone Synthase and Steroid 11β-Hydroxylase Deficiencies

Hormone Research in Paediatrics, 1999
The most potent corticosteroids are 11β-hydroxylated compounds. In humans, two cytochrome P450 isoenzymes with 11β-hydroxylase activity, catalysing the biosynthesis of cortisol and aldosterone, are present in the adrenal cortex. CYP11B1, the gene encoding 11β-hydroxylase (P450c11), is expressed on high levels in the zona fasciculata and is regulated by
Peter, M, Dubuis, Jean-Michel Marie Maurice, Sippell, WG   +2 more
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