Results 71 to 80 of about 509,885 (240)

CRISPR targeting of FOXL2 c.402C>G mutation reduces malignant phenotype in granulosa tumor cells and identifies anti‐tumoral compounds

Molecular Oncology, Volume 19, Issue 4, Page 1092-1116, April 2025.
FOXL2 c.402C>G mutation drives granulosa cell tumors. Using CRISPR technology, we selectively corrected this mutation, reducing malignancy and increasing sensitivity to dasatinib and ketoconazole. Transcriptomic changes revealed potential therapeutic targets, demonstrating CRISPR's promise for treating this rare ovarian cancer.
Sandra Amarilla‐Quintana, Paloma Navarro, Iván Hernández, Alejandra Ramos, Ana Montero‐Calle, Pablo Cabezas‐Sainz, Maria J Barrero, Diego Megías, Borja Vilaplana‐Martí, Carolina Epifano, Déborah Gómez‐Dominguez, Sara Monzón, Isabel Cuesta, Laura Sánchez, Rodrigo Barderas, Jesús García‐Donas, Alberto Martín, Ignacio Pérez de Castro   +17 more
wiley   +1 more source

Multi‐Omics Analysis Reveals Impacts of LincRNA Deletion on Yeast Protein Synthesis

Advanced Science, Volume 12, Issue 13, April 3, 2025.
This study demonstrates that the deletion of specific lincRNAs in yeast affects protein secretion. An engineered yeast strain, with multiple genetic modifications including lincRNA deletion, achieves a high yield of 4.1 g L⁻1 in fed‐batch cultivation.
Ling Qin, Yuyang Pan, Songlyu Xue, Zhibo Yan, Chufan Xiao, Xiufang Liu, Dan Yuan, Jin Hou, Mingtao Huang   +8 more
wiley   +1 more source

Modeling the dynamics of hypoxia inducible factor-1α (HIF-1α) within single cells and 3D cell culture systems [PDF]

arXiv, 2014
HIF (Hypoxia Inducible Factor) is an oxygen-regulated transcription factor that mediates the intracellular response to hypoxia in human cells. There is increasing evidence that cell signaling pathways encode temporal information, and thus cell fate may be determined by the dynamics of protein levels.
arxiv  

Advances in Fecal Microbiota Transplantation for Gut Dysbiosis‐Related Diseases

Advanced Science, Volume 12, Issue 13, April 3, 2025.
This article reviews the use of fecal microbiota transplantation (FMT) to treat intestinal diseases like recurrent Clostridioides difficile infection (rCDI), inflammatory bowel disease (IBD), and irritable bowel syndrome (IBS). It discusses FMT's efficacy, influencing factors such as donor selection, recipient characteristics, and treatment protocols ...
Shuna Hou, Jiachen Yu, Yongshuang Li, Duoyi Zhao, Zhiyu Zhang   +4 more
wiley   +1 more source

Gonadal Location of the Enzymes Steroid 11β- and 21-Hydroxylases

, 1975
Experimental evidence concerning the presence of the corticosteroidogenic enzymes steroid 11β- and 21-hydroxylases in the gonads of vertebrates, echinoderms, crustaceans and molluscs is reviewed.
L. Colombo, P. Belvedere
semanticscholar   +1 more source

Adrenal High‐Expressional CYP27A1 Mediates Bile Acid Increase and Functional Impairment in Adult Male Offspring by Prenatal Dexamethasone Exposure

Advanced Science, Volume 12, Issue 14, April 10, 2025.
Prenatal dexamethasone exposure (PDE) can induce dysfunction in adrenal steroid synthesis in adult male offspring. This study demonstrate that the mechanism is associated with the upregulation of fetal adrenal cholesterol 27 hydroxylase (CYP27A1)promoter region histone 3 lysine 27 acetylation (H3K27ac) levels and expression mediated by PDE through the ...
Jiangang Cao, Wen Hu, Yawen Chen, Aihemaitijiang Ailikaiti, Ziyi Zhang, Lingbo Rong, Hong Yu, Hui Wang   +7 more
wiley   +1 more source

Asymmetric Regulation on Steroids: U.S. Competition Policy and Fiber to the Home [PDF]

arXiv, 2001
Fiber to the Home (FTTH) describes a set of emerging technologies with the potential to affect competition in local access. On one hand, the high cost of deploying fiber to the residence suggests limitations on facilities-based competition among FTTH networks.
arxiv  

Prenatal Diagnosis and Treatment of Steroid 21-Hydroxylase Deficiency

Clinical Pediatric Endocrinology, 2008
Steroid 21-hydroxylase deficiency (21-OHD) accounts for 90-95% of congenital adrenal hyperplasia (CAH) cases. It is classified into three distinct clinical phenotypes: the salt-wasting (SW), simple virilizing (SV) and nonclassical forms (NC). As girls with the SW and SV forms of 21-OHD are exposed to high systemic levels of adrenal androgens during ...
Toshihiro Tajima, Kenji Fujieda
openaire   +4 more sources

Progesterone Enhances Sensitivity of Ovarian Cancer Cells to SN38 Through Inhibition of Topoisomerase I and Inducing Ferroptosis

Cancer Reports, Volume 8, Issue 4, April 2025.
ABSTRACT Background Progesterone rapidly induces ovarian cancer cell death through non‐genomic actions mediated by the membrane progesterone receptor (mPR). Aims We investigated the combined effects of progesterone and SN38, an active metabolite of irinotecan, on ovarian cancer cells. Methods and Results mPR‐positive and PR‐negative ovarian cancer cell
Takahiro Koyanagi, Yasushi Saga, Yoshifumi Takahashi, Kohei Tamura, Eri Suizu, Suzuyo Takahashi, Akiyo Taneichi, Yuji Takei, Hiroaki Mizukami, Hiroyuki Fujiwara   +9 more
wiley   +1 more source

Identification of a Gene Encoding a New Fungal Steroid 7-Hydroxylase and Its Functional Characterization in Pichia pastoris Yeast. [PDF]

Int J Mol Sci, 2023
Kollerov V, Tarlachkov S, Shutov A, Kazantsev A, Donova M.   +4 more
europepmc   +1 more source