Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Feeding frequency and stocking density are important factors affecting growth and maturation of cultured fishes. The aims of this investigation were to determine the effects of stocking density (10, 20, 30 and 40 fish per aquarium) and feeding frequency (
Mohammadreza Imanpour +3 more
doaj
DERIVATION AND OPTIMIZATION OF A STOCHASTIC LIVESTOCK WEIGHT GAIN RESPONSE TO STOCKING DENSITY MODEL [PDF]
Dual-purpose winter wheat production is an important economic enterprise in the southern Great Plains of the United States. Because of the complex interactions involved in producing wheat grain and beef gain from a single crop, stocking density is an ...
Brorsen, B. Wade +5 more
core +1 more source
Comparison of milk production from clover-based and fertilizer-N-based grassland [PDF]
peer-reviewedFunding for this study was provided by the Irish National Development Plan and the Irish Dairy Levy.This study, conducted over four years (2003–2006), compared herbage production, nutritive value of herbage, the length of the grazing season ...
Casey, I.A. +2 more
core
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
The Effects of Stocking Density on the Growth and Survival Rate of Ompok Hypophthalmus Reared in Fish Cages [PDF]
The research was conducted from April to June 2010, in the research pondsof the Fisheries and Marine Science Faculty, Riau University. The objectives ofthis research were to find out the effects of different stocking density on thegrowth and survival ...
Hs, N. (Nuraini) +2 more
core
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
DETERMINATION OF THE BEST STOCKING DENSITY FOR REARING HUSO HUSO
Effects of stocking density on growth, food conversion ratio, specific growth rate, percentage of body weight increase in Huso huso were studied in two phases. Juveniles below one year old with an average weight of 92.09±1.72 grams and stocking densities
M. Mohseni; H. Porali; M. Sajadi; V. Aghtoman
doaj
An evaluation of the survival of Atlantic salmon (Salmo salar L.)fry stocked in eight streams in the North West of England. [PDF]
Eight streams from the North West of England were stocked with Atlantic salmon (Salmo salar L.) fed fry at densities ranging from 1 to 4/m2 over a period of up to three years to evaluate survival to the end of the first an d second growing periods and ...
Aprahamian, M.W., Farooqi, M.A.
core
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley +1 more source

