Results 161 to 170 of about 7,802 (179)

Ribosomal A-site interactions with near-cognate tRNAs drive stop codon readthrough

Nature Structural & Molecular Biology, 2023
SUMMARYtRNAs serve as a dictionary for the ribosome translating the genetic message from mRNA into a polypeptide chain. Besides this canonical role, tRNAs are involved in other processes like programmed stop codon readthrough (SC-RT). There, tRNAs with near-cognate anticodons to stop codons must outcompete release factors and incorporate into the ...
Zuzana Čapková Pavlíková, Petra Miletínová, Adriana Roithová, Klára Pospíšilová, Kristína Záhonová, Ambar Kachale, Thomas Becker, Ignacio M. Durante, Julius Lukeš, Zdeněk Paris, Petra Beznosková, Leoš Shivaya Valášek   +11 more
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Gentamicin‐induced readthrough of stop codons in duchenne muscular dystrophy

Annals of Neurology, 2010
Abstract Objective The objective of this study was to establish the feasibility of long‐term gentamicin dosing to achieve stop codon readthrough and produce full‐length dystrophin. Mutation suppression of stop codons, successfully achieved in the mdx
Vinod, Malik, Louise R, Rodino-Klapac, Laurence, Viollet, Cheryl, Wall, Wendy, King, Roula, Al-Dahhak, Sarah, Lewis, Christopher J, Shilling, Janaiah, Kota, Carmen, Serrano-Munuera, John, Hayes, John D, Mahan, Katherine J, Campbell, Brenda, Banwell, Majed, Dasouki, Victoria, Watts, Kumaraswamy, Sivakumar, Ricardo, Bien-Willner, Kevin M, Flanigan, Zarife, Sahenk, Richard J, Barohn, Christopher M, Walker, Jerry R, Mendell   +22 more
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Polypeptide chain termination and stop codon readthrough on eukaryotic ribosomes

Reviews of Physiology, Biochemistry and Pharmacology, 2005
During protein translation, a variety of quality control checks ensure that the resulting polypeptides deviate minimally from their genetic encoding template. Translational fidelity is central in order to preserve the function and integrity of each cell. Correct termination is an important aspect of translational fidelity, and a multitude of mechanisms
Rospert, Sabine, Rakwalska, M., Dubaquie, Yves P.   +2 more
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Kinetics of Translating Ribosomes Determine the Efficiency of Programmed Stop Codon Readthrough

Journal of Molecular Biology, 2023
During translation, a stop codon on the mRNA signals the ribosomes to terminate the process. In certain mRNAs, the termination fails due to the recoding of the canonical stop codon, and ribosomes continue translation to generate C-terminally extended protein. This process, termed stop codon readthrough (SCR), regulates several cellular functions.
Debaleena Kar, Debraj Manna, Lekha E. Manjunath, Anumeha Singh, Saubhik Som, Kirtana Vasu, Sandeep M. Eswarappa   +6 more
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Transcriptome-wide investigation of stop codon readthrough in Saccharomyces cerevisiae

2020
Translation of mRNA into a polypeptide is terminated when the release factor eRF1 recognizes a UAA, UAG, or UGA stop codon in the ribosomal A site and stimulates nascent peptide release. However, “stop codon readthrough” can occur when a near-cognate tRNA outcompetes eRF1 in decoding the stop codon, resulting in the continuation of elongation into the ...
Mangkalaphiban, Kotchaphorn, He, Feng, Ganesan, Robin, Wu, Chan, Jacobson, Allan   +4 more
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Factors impacting the aminoglycoside-induced UGA stop codon readthrough in selenoprotein translation

Journal of Trace Elements in Medicine and Biology, 2016
Aminoglycosides (AG) are oligosaccharide antibiotics that interfere with the small ribosomal subunit in aerobic, Gram-negative bacteria, causing pathogen-destructing error rates in their protein biosynthesis. Aminoglycosides also induce mRNA misinterpretation in eukaryotic cells, especially of the UGA (Opal)-stop codon, albeit to a lower extent.
Janine, Martitz, Peter Josef, Hofmann, Jörg, Johannes, Josef, Köhrle, Lutz, Schomburg, Kostja, Renko   +5 more
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Readthrough strategies for stop codons in Duchenne muscular dystrophy.

Acta myologica : myopathies and cardiomyopathies : official journal of the Mediterranean Society of Myology, 2006
Duchenne muscular dystrophy is due to mutations of the dystrophin gene. These are large deletions or duplications in 80% of cases, while premature stop codons (nonsense point mutations) account for 7% of cases. This subgroup of patients may take advantage of the properties of the antibiotic gentamicin to suppress stop codons (readthrough).
AURINO S, NIGRO, Vincenzo
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Widespread readthrough events in plants reveal unprecedented plasticity of stop codons

2023
AbstractStop codon readthrough (SCR), the decoding of a stop codon as a sense codon by the ribosome, has important biological implications but remains largely uncharacterized in plants. Here, we identified 1,009 SCR events in two monocots (maize, rice) and two dicots (soybean,Arabidopsis) using a proteogenomic strategy with 80 customized databases. SCR
Yuqian Zhang, Hehuan Li, Yanting Shen, Shunxi Wang, Lei Tian, Haoqiang Yin, Jiawei Shi, Anqi Xing, Jinghua Zhang, Usman Ali, Abdul Sami, Xueyan Chen, Chenxuan Gao, Yangtao Zhao, Yajing Lyu, Xiaoxu Wang, Yanhui Chen, Zhixi Tian, Shu-Biao Wu, Liuji Wu   +19 more
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Transcript-specific induction of stop codon readthrough using CRISPR-dCas13 system

2023
AbstractStop codon readthrough (SCR) is the process where translation continues beyond a stop codon on an mRNA. Here, we describe a strategy to enhance or induce SCR in a transcript-selective manner using CRISPR-dCas13 system. Using specific guide RNAs, we targeted dCas13 to the downstream region of the canonical stop codons of mammalianAGO1andVEGFA ...
Lekha E. Manjunath, Anumeha Singh, Debaleena Kar, Karthi Sellamuthu, Sandeep M. Eswarappa   +4 more
openaire   +1 more source

Deciphering the molecular mechanism of stop codon readthrough

Biological Reviews, 2021
Martine Palma, Fabrice Lejeune
exaly