Defining the high-translational readthrough stop codon context. [PDF]
PLoS GeneticsTranslational termination is not entirely efficient and competes with elongation, which might result in translational readthrough (TR). TR occurs when a near-cognate tRNA binds to a stop codon, (mis)interpreting it as a sense codon and producing a C ...
Daniela Smoljanow +3 more
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Stop Codon Context-Specific Induction of Translational Readthrough [PDF]
Biomolecules, 2021 Premature termination codon (PTC) mutations account for approximately 10% of pathogenic variants in monogenic diseases. Stimulation of translational readthrough, also known as stop codon suppression, using translational readthrough-inducing drugs (TRIDs)
Mirco Schilff +3 more
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Extended stop codon context predicts nonsense codon readthrough efficiency in human cells [PDF]
Nature CommunicationsProtein synthesis terminates when a stop codon enters the ribosome’s A-site. Although termination is efficient, stop codon readthrough can occur when a near-cognate tRNA outcompetes release factors during decoding.
Kotchaphorn Mangkalaphiban +6 more
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Distal nucleotides affect the rate of stop codon read‐through [PDF]
Quantitative Biology, 2023 Background A key step in gene expression is the recognition of the stop codon to terminate translation at the correct position. However, it has been observed that ribosomes can misinterpret the stop codon and continue the translation in the 3′UTR region.
Luciana I. Escobar +3 more
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Thermodynamic and kinetic insights into stop codon recognition by release factor 1. [PDF]
PLoS ONE, 2014 Stop codon recognition is a crucial event during translation termination and is performed by class I release factors (RF1 and RF2 in bacterial cells). Recent crystal structures showed that stop codon recognition is achieved mainly through a network of ...
Krista Trappl +2 more
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Environment modulates protein heterogeneity through transcriptional and translational stop codon readthrough [PDF]
Nature CommunicationsStop codon readthrough events give rise to longer proteins, which may alter the protein’s function, thereby generating short-lasting phenotypic variability from a single gene.
Maria Luisa Romero Romero +6 more
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Adaptation of the short intergenic spacers between co-directional genes to the Shine-Dalgarno motif among prokaryote genomes [PDF]
BMC Genomics, 2009 Background In prokaryote genomes most of the co-directional genes are in close proximity. Even the coding sequence or the stop codon of a gene can overlap with the Shine-Dalgarno (SD) sequence of the downstream co-directional gene.
Romeu Antoni +2 more
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Stop codons in bacteria are not selectively equivalent [PDF]
Biology Direct, 2012 Background The evolution and genomic stop codon frequencies have not been rigorously studied with the exception of coding of non-canonical amino acids.
Povolotskaya Inna S +3 more
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eLife, 2020 Stop codon readthrough (SCR) occurs when the ribosome miscodes at a stop codon. Such readthrough events can be therapeutically desirable when a premature termination codon (PTC) is found in a critical gene.
Jamie R Wangen, Rachel Green
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Transcript-specific induction of stop codon readthrough using a CRISPR-dCas13 system [PDF]
EMBO ReportsStop codon readthrough (SCR) is the process where translation continues beyond a stop codon on an mRNA. Here, we describe a strategy to enhance or induce SCR in a transcript-selective manner using a CRISPR-dCas13 system.
Lekha E Manjunath +6 more
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