Results 21 to 30 of about 270,897 (278)
Translation initiation factor eIF3 promotes programmed stop codon readthrough. [PDF]
, 2015 Programmed stop codon readthrough is a post-transcription regulatory mechanism specifically increasing proteome diversity by creating a pool of C-terminally extended proteins.
Beznoskova, Petra +4 more
core +1 more source
Therapeutics Based on Stop Codon Readthrough [PDF]
Annual Review of Genomics and Human Genetics, 2014 Nonsense suppression therapy encompasses approaches aimed at suppressing translation termination at in-frame premature termination codons (PTCs, also known as nonsense mutations) to restore deficient protein function. In this review, we examine the current status of PTC suppression as a therapy for genetic diseases caused by nonsense mutations.
Kim M, Keeling +3 more
openaire +2 more sources
Severe congenital microcephaly with AP4M1 mutation, a case report [PDF]
, 2017 Background: Autosomal recessive defects of either the B1, E1, M1 or S1 subunit of the Adaptor Protein complex-4 (AP4) are characterized by developmental delay, severe intellectual disability, spasticity, and occasionally mild to moderate microcephaly of ...
Abramowicz, Marc +6 more
core +2 more sources
Repurposing tRNAs for nonsense suppression
Nature Communications, 2021 Here, the authors report de novo design, optimization and characterization of tRNAs that decode UGA stop codons in E. coli. The structure of the ribosome in a complex with the designed tRNA bound to a UGA stop codon suggests that distinct A-site ligands (
Suki Albers +10 more
doaj +1 more source
MATEC Web of Conferences, 2018 In this paper, the influence of the combination of last sense codon and stop codon on expression efficiency was studied. By our study, for the last sense codon CCG, the RFI of GFP(CCG) was 2.1 fold when the stop codon was UAA, but in comparison, the RFI ...
Zhang Xing +5 more
doaj +1 more source
An engineered Tetrahymena tRNA(Gln) for in vivo incorporation of unnatural amino acids into proteins by nonsense suppression [PDF]
, 1996 A new tRNA, THG73, has been designed and evaluated as a vehicle for incorporating unnatural amino acids site-specifically into proteins expressed in vivo using the stop codon suppression technique.
Abelson, John N. +7 more
core +1 more source
In Vitro Selection of mRNA Display Libraries Containing an Unnatural Amino Acid [PDF]
, 2002 The incorporation of unnatural amino acid into selectable, amplifiable peptide and protein libraries expands the chemical diversity of such libraries, thus considerably facilitating the process of obtaining ligands with improved properties (affinity ...
Li, Shuwei +2 more
core +1 more source
Pyrrolysine Amber Stop‐Codon Suppression: Development and Applications [PDF]
ChemBioChem, 2017 AbstractThe pyrrolysine tRNA synthetase‐tRNA pair is probably one of the most promiscuous tRNA–synthetase pairs found in nature, capable of genetically encoding a plethora of noncanonical amino acids through stop codon reassignment. Proteins containing reactive handles, post‐translational modification mimics or both can be produced in practical ...
Robin Brabham, Martin A. Fascione
openaire +3 more sources
Stop-codon read-through arises largely from molecular errors and is generally nonadaptive.
PLoS Genetics, 2019 Stop-codon read-through refers to the phenomenon that a ribosome goes past the stop codon and continues translating into the otherwise untranslated region (UTR) of a transcript.
Chuan Li, Jianzhi Zhang
doaj +1 more source
A condition-specific codon optimization approach for improved heterologous gene expression in Saccharomyces cerevisiae [PDF]
, 2014 All authors are with the Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712, USA -- Hal S.
Alper, Hal S. +3 more
core +3 more sources