Synthetic Approach to Stop-Codon Scanning Mutagenesis [PDF]
Journal of the American Chemical Society, 2011 A general combinatorial mutagenesis strategy using common dimethoxytrityl-protected mononucleotide phosphoramidites and a single orthogonally protected trinucleotide phosphoramidite (Fmoc-TAG; Fmoc = 9-fluorenylmethoxycarbonyl) was developed to scan a gene with the TAG amber stop codon with complete synthetic control.
Lihua, Nie +4 more
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Selection for tandem stop codons in ciliate species with reassigned stop codons
PLOS ONE, 2019 The failure of mRNA translation machinery to recognize a stop codon as a termination signal and subsequent translation of the 3' untranslated region (UTR) is referred to as stop codon readthrough, the frequency of which is related to the length, composition, and structure of mRNA sequences downstream of end-of-gene stop codons.
Ira Fleming, Andre R. O. Cavalcanti
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Stop codon reassignments in the wild
Science, 2014 The canonical genetic code is assumed to be deeply conserved across all domains of life with very few exceptions. By scanning 5.6 trillion base pairs of metagenomic data for stop codon reassignment events, we detected recoding in a substantial fraction of the >1700 environmental samples examined. We observed extensive opal
ivanova, Natalia +9 more
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Karyorelict ciliates use an ambiguous genetic code with context-dependent stop/sense codons
Peer Community Journal, 2022 In ambiguous stop/sense genetic codes, the stop codon(s) not only terminate translation but can also encode amino acids. Such codes have evolved at least four times in eukaryotes, twice among ciliates (Condylostoma magnum and Parduczia sp.).
Seah, Brandon Kwee Boon +2 more
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Directional Next-Generation RNA Sequencing and Examination of Premature Termination Codon Mutations in Endoglin/Hereditary Haemorrhagic Telangiectasia [PDF]
, 2012 Hereditary haemorrhagic telangiectasia (HHT) is a disease characterised by abnormal vascular structures, and most commonly caused by mutations in ENG, ACVRL1 or SMAD4 encoding endothelial cell-expressed proteins involved in TGF-β superfamily signalling ...
Begbie, ME +6 more
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Evolutionary Dynamics of Abundant Stop Codon Readthrough [PDF]
Molecular Biology and Evolution, 2016 Translational stop codon readthrough emerged as a major regulatory mechanism affecting hundreds of genes in animal genomes, based on recent comparative genomics and ribosomal profiling evidence, but its evolutionary properties remain unknown. Here, we leverage comparative genomic evidence across 21 Anopheles mosquitoes to systematically annotate ...
Fields, Gabriel +5 more
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Versatile dual reporter gene systems for investigating stop codon readthrough in plants. [PDF]
PLoS ONE, 2009 Translation is most often terminated when a ribosome encounters the first in-frame stop codon (UAA, UAG or UGA) in an mRNA. However, many viruses (and some cellular mRNAs) contain "stop" codons that cause a proportion of ribosomes to terminate and others
Nga T Lao +3 more
doaj +1 more source
Codon-biased translation can be regulated by wobble-base tRNA modification systems during cellular stress responses [PDF]
, 2014 tRNA (tRNA) is a key molecule used for protein synthesis, with multiple points of stress-induced regulation that can include transcription, transcript processing, localization and ribonucleoside base modification.
Begley, Thomas J. +2 more
core +1 more source
Selection Shapes Synonymous Stop Codon Use in Mammals [PDF]
Journal of Molecular Evolution, 2019 AbstractPhylogenetic models of the evolution of protein-coding sequences can provide insights into the selection pressures that have shaped them. In the application of these models synonymous nucleotide substitutions, which do not alter the encoded amino acid, are often assumed to have limited functional consequences and used as a proxy for the neutral
Seoighe, Cathal +4 more
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Statistical analysis of readthrough levels for nonsense mutations in mammalian cells reveals a major determinant of response to gentamicin. [PDF]
PLoS Genetics, 2012 The efficiency of translation termination depends on the nature of the stop codon and the surrounding nucleotides. Some molecules, such as aminoglycoside antibiotics (gentamicin), decrease termination efficiency and are currently being evaluated for ...
Célia Floquet +3 more
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