FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Influence of variety, toppling stage, and storage duration on postharvest quality and shelf life of onion(Allium cepa L.) in Bahir Dar Zuria district. [PDF]
Sci RepAssefa F +3 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Enhancing fruit quality attributes and storability of Ruby Seedless grape cultivar using different irradiated potassium sources as an alternative to ethrel. [PDF]
BMC BiotechnolAhmed MF, Fekry OM, Salama BSEA.
europepmc +1 more source
FEBS Open Bio, EarlyView.14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa +2 more
wiley +1 more source
Integrating QTL mapping, BSA-seq and RNA-seq to identify candidate genes regulating seed storability from Dongxiang wild rice. [PDF]
Front Plant SciZhou S, Wu T, Wang S, He J, Hu B.
europepmc +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Editorial: postharvest ripening, senescence, and technology, Volume II. [PDF]
Front GenetDeltsidis A, Liu T, Albornoz K.
europepmc +1 more source
Storable types: free, absorbing, custom
The Store library (Noûs and Scherer, 2023; Allain, Clément, Moine, and Scherer, 2024) provides a store data structure that tracks a bag of mutable references, and provides an efficient way to capture its current state and restore it later on. This is useful to implement backtracking algorithms over mutable state, as found in type-checkers and automated
Clément, Basile +2 more
openaire +1 more source