Results 71 to 80 of about 2,257,308 (283)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

New Strain Gradient Theory And Analysis

, 2009
A new strain gradient theory which is based on energy nonlocal model is proposed in this paper, and the theory is applied to investigate the size effects in thin metallic wire torsion, ultra-thin beam bending and micro-indentation of polycrystalline ...
易大可, 王自强, 陈少华, Wang TC
core   +2 more sources

One-Dimensional Large-Strain Nonlinear Consolidation of Overconsolidated Clays with a Threshold Hydraulic Gradient

Advances in Civil Engineering, 2018
The existence of the threshold hydraulic gradient in clays under a low hydraulic gradient has been recognized by many studies. Meanwhile, most nature clays to some extent exist in an overconsolidated state more or less.
Chuanxun Li, Jinyang Xiao, Yang Yang, Wenbing Wu   +3 more
doaj   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Using Rubber Fillers to Fabricate Strain Gradient Material—An Excellent Approach for Developing High‐Toughness Cement‐Based Composites

Engineering Reports
Improving the toughness and crack resistance of cement‐based materials by using strain gradient design is beneficial for enhancing the durability of structures and fully utilizing advantages of various attribute materials, thus reducing life cycle costs ...
Keliang Wang, Chuanli Zhong, Zhide Huang, Yueqiang Qi, Gangnian Xu   +4 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Interface crack problems with strain gradient effects

, 2002
In this paper, the strain gradient theory proposed by Chen and Wang (2001 a, 2002b) is used to analyze an interface crack tip field at micron scales.
Chen, SH (reprint author), Chinese Acad Sci, Inst Mech, LNM, Beijing 100080, Peoples R China., 陈少华, 王自强   +2 more
core   +1 more source

Strain gradient induced vortex-like domain evolution in freestanding ferroelectric membranes

Nano Research
Ferroelectric vortex domains in strained ferroelectric membranes have recently garnered significant scientific interest. However, understanding domain evolution under varying strain conditions has been challenging due to experimental limitations in ...
Butong Zhang, Guangming Lu, Wanbo Qu, Guohua Dong, Shishun Zhao, Minye Yang, Guannan Yang, Zhengwei Tao, Bin Peng, Yanan Zhao, Zhongqiang Hu, Zhiguang Wang, Yang Zhang, Haijun Wu, Suzhi Li, Zuo-Guang Ye, Xiangdong Ding, Ming Liu   +17 more
doaj   +1 more source