Results 51 to 60 of about 812,407 (248)
TPR2 is required for efficient strand-displacement.
, 2012 Strand displacement was assessed using a set of 3 oligonucleotides with gaps of 1, 3 and 6 nt respectively. After incubation at the indicated times the reaction mixtures were run on a 18% denaturing polyacrylamide gel. Reactions were carried out in 20 µl
Cesar S. Cardona-Felix (115739) +3 more
core +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
8-Bit Adder and Subtractor with Domain Label Based on DNA Strand Displacement
Molecules, 2018 DNA strand displacement, which plays a fundamental role in DNA computing, has been widely applied to many biological computing problems, including biological logic circuits.
Weixuan Han, Changjun Zhou
doaj +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
DNA Replication Through Strand Displacement During Lagging Strand DNA Synthesis in Saccharomyces cerevisiae [PDF]
, 2019 This review discusses a set of experimental results that support the existence of extended strand displacement events during budding yeast lagging strand DNA synthesis.
M. Giannattasio +5 more
core +1 more source
A Double-Layer Image Encryption Scheme Based on Chaotic Maps and DNA Strand Displacement
Journal of Chemistry, 2022 The image encryption schemes combining chaotic maps, DNA coding, and DNA sequence operation can effectively protect the image. In this paper, a double-layer image encryption scheme is proposed by combining chaotic maps with DNA strand displacement (DSD).
Zhen Tang +5 more
doaj +1 more source
Frontiers in Microbiology, 2022 Viruses are the most abundant and diverse biological entities on the planet and constitute a significant proportion of Earth’s genetic diversity. Most of this diversity is not represented by isolated viral-host systems and has only been observed through ...
Rachel A. Keown +10 more
doaj +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy
FEBS Letters, EarlyView.The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley +1 more source
Switching the activity of Cas12a using guide RNA strand displacement circuits
Nature Communications, 2019 Cas12a is a useful alternative to Cas9 for genome editing and regulation. Here the authors design strand displacement gRNAs that can add functionality to Cas12a by acting as multi-input logic gates.
Lukas Oesinghaus, Friedrich C. Simmel
doaj +1 more source